A drug for preventing and controlling rice blast fungus
A technology of rice blast fungus and medicine, which is applied in the field of drugs for the prevention and control of rice blast fungus, can solve the problems of pesticide residues, loss of resistance, time-consuming and labor-intensive anti-bacteria, etc., and achieve good application value.
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Embodiment 1
[0025] 1 Materials and methods
[0026] 1.1 Test strains
[0027] Field strain B157 of Magnaporthe grisea was donated by Naweed I.Naqvi Laboratory of Temasek Institute of Life Sciences, Singapore; Agrobacterium strain AG1 was preserved by our laboratory.
[0028] 1.2 Test plants
[0029] The susceptible rice LTH was donated by Dr. Jiang Liqun from Guangdong Rice Institute.
[0030] 4. Tangeretin (Sigama, T8951).
[0031] 1.3 Main instruments
[0032] Optical microscope (OLYMPUS)
[0033] 1.4 The formulation of the culture medium
[0034] 1) Prune-agar (PA) (1L) medium for culture and sporulation of Magnaporthe grisea: 40ml Prune fruit juice, 2.5g lactose, 2.5g sucrose, 1g yeast extract, 20g agar powder, adjust the pH to 6.5 with 10M NaOH.
[0035] 1.5 Sensitivity detection of conidia of Magnaporthe grisea to tangeretin
[0036] Collect the conidia of the PA plate culture strain and adjust the concentration to 10 5 pcs / ml, 200 μM tangeretin to test the sensitivity of co...
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