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Biological control method for rice blast

A rice blast and rice technology, applied in the field of biological control of rice pests and diseases, can solve the problems of reduced control efficiency, complex genetic background, and reduced rice quality, and achieve the effect of biological control

Inactive Publication Date: 2018-04-27
安徽省中日农业环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex genetic background of the rice blast fungus, it is easy to mutate, and it is difficult for the disease-resistant varieties to keep up with the variation speed of the physiological races of the pathogenic bacteria, which often leads to the loss of resistance of a new disease-resistant rice variety after a few years of planting.
However, chemical control will not only pollute the environment, reduce the quality of rice, and destroy the ecology, but also lead to the continuous increase of drug resistance of bacteria and reduce the control effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A biological control method for rice blast, comprising the following steps:

[0022] Step 1: Select healthy rice leaves, cut them into 5cm leaf segments, put them into a triangular flask with 75% ethanol, shake and wash twice, pour off the ethanol, add sterile water to soak for 30 minutes, and then make a gradient dilution to obtain a gradient dilution liquid;

[0023] Step 2: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;

[0024] Step 3: Draw the gradient dilution and spread it on the NA solid medium. After drying, culture it upside down in a constant temperature incubator at 30°C for 48 hours, pick a single colony, and draw a gradient line on the NA solid medium to obtain a single colony again. Bacterial colonies, the obtained single colonies are preserved in the slan...

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PUM

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Abstract

The invention relates to a biological control method for rice blast and relates to the technical field of biological control of rice pests and diseases. The preparation method comprises preparing a gradient diluent, preparing a NA solid medium, separating bacilli, preparing an LB liquid culture solution, preparing a crude extract protein liquid and carrying out rice colonization. The isolated bacilli can secrete antibacterial substances to inhibit the growth of pathogenic bacteria and induce the plant defense system to resist invasion of pathogenic bacteria. The tested strain has remarkable effects of inhibiting the germination of the magaporthe grisea conidiophores, the prolongation of germ tubes and the formation of appressorium. The active substances of the strain have a strong inhibitory effect on magaporthe grisea and a magaporthe grisea mycelium growth inhibition rate of 80% or more.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a method for biological control of rice blast. Background technique [0002] Rice is China's largest grain crop, and its output accounts for about 40% of China's grain output. The three major diseases of rice include rice blast, bacterial blight and rice sheath blight. Among them, rice blast is one of the most threatening diseases in rice production. It is caused by the fungus Pyrospora fungus and can occur throughout the rice development period. Due to the rapid prevalence of the disease, under suitable environmental conditions, it can cause a pandemic in a short period of time and bring huge losses to rice production. At present, in agriculture, disease-resistant breeding and chemical agents are mainly used to gradually control rice blast. However, due to the complex genetic background of Magnaporthe grisea, it is easy to mutate, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00C07K1/36C07K1/34C07K1/30A01N63/02A01N63/00A01P21/00A01P3/00A01C1/00A01G22/22C12R1/07
CPCA01C1/00A01N63/00A01N63/10C07K1/303C07K1/34C07K1/36C12N1/20C12P21/00
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司
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