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Method for lowering sensitivity of pyricularia grisea effect protein gene overexpression strain to pH

A technology of gene overexpression and effector protein, which is applied in the field of reducing the pH sensitivity of rice blast fungus effector protein gene overexpression strains, and can solve the problems of fungal secretion of metabolites or negative effects of lyase

Inactive Publication Date: 2017-05-31
YUNNAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Loss of PACC has a greatly negative effect on fungal secretion of metabolites or lytic enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare medium with pH5 and pH8, inoculate the mycelium of the overexpressed strain on the medium, wait for about 10 days to count the number of spores produced, and treat the spores of the overexpressed strain with pH5 and pH8 at the same time, observe the spore germination rate and appressoria formation rate, etc. (Table 2).

[0024] Table 1 Sporulation rate, germination rate and appressoria formation rate of spores of rice blast fungus effector protein overexpressed strains treated at pH5

[0025] category Sporulation rate (%) Germination rate (%) Appressoria formation rate (%) Treatment (pH5) 97.6 95.8 98.6 Control ① 85.6 84.3 79.8

[0026] Table 2 Sporulation rate, germination rate and appressoria formation rate of spores of rice blast fungus effector protein overexpressed strains treated at pH8

[0027] category Sporulation rate (%) Germination rate (%) Appressoria formation rate (%) Treatment (pH8) 94.6 96...

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Abstract

The invention discloses a method for lowering sensitivity of a pyricularia grisea effect protein gene overexpression strain to pH. The method comprises the following steps: treating pyricularia grisea effect protein gene overexpression strain spores with pH5 and pH8; performing statistics to obtain spore yield, sprouting rate and appressorium forming rate. Compared with the prior art, the method has the advantages that the sensitivity of the pyricularia grisea effect protein gene overexpression strain to different pH can be easily, conveniently and accurately determined, and an important reference basis is laid for rapid comprehension of the pathogenicity of a pathogenic bacteria effect protein gene overexpression strain.

Description

technical field [0001] The invention belongs to the field of plant protection and biotechnology, and in particular relates to a method for reducing pH sensitivity of blast fungus effector protein gene overexpression strains. Background technique [0002] Some fungi have evolved complex regulatory mechanisms to recognize and respond to pH changes around them during their persistent interactions with their environment. In addition, some fungi secrete different proteins depending on the pH of their surroundings. In order to successfully invade the host, Botrytis cinerea secretes different proteins in different tissues of the host during the infection process. Although only a few 'true' virulence factors are successful in suppressing host defense responses, different extracellular secreted proteins are required to act in concert. Seven proteins in Aspergillus nidulans are related to pH recognition ( et al., 2008), two of the proteins, PalH and Pail, are putative membrane pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/14C12R1/645
CPCC12N1/14C12N1/36
Inventor 杨静李成云王春梅刘林吴奇王云锋
Owner YUNNAN AGRICULTURAL UNIVERSITY
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