Preparation method of microbial pesticide for controlling rice blast

Abstract

The invention discloses a preparation method of a microbial pesticide for controlling rice blast, and relates to the technical field of biological control of rice diseases and pest insects; the preparation method includes the steps: preparation of a gradient dilution liquid, preparation of an NA solid culture medium, bacillus separation, preparation of a PDA culture medium, and preparation of a spore liquid. Bacillus obtained from isolation can secrete antibacterial substances to inhibit pathogenic bacteria growth, and induces a plant defense system against pathogenic bacteria invasion; a tested strain has a significant inhibitory effect on germination of magnaporthe grisea conidiospores, elongation of germ tubes and appressorium formation; the strain active substances have a relatively strong role in inhibiting magnaporthe grisea, and the magnaporthe grisea mycelium growth inhibition rate reaches 80% or more.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a preparation method of microbial pesticides for preventing and controlling rice blast. Background technique [0002] Rice is China's largest grain crop, and its output accounts for about 40% of China's grain output. The three major diseases of rice include rice blast, bacterial blight and rice sheath blight. Among them, rice blast is one of the most threatening diseases in rice production. It is caused by the fungus Pyrospora fungus and can occur throughout the rice development period. Due to the rapid prevalence of the disease, under suitable environmental conditions, it can cause a pandemic in a short period of time and bring huge losses to rice production. At present, in agriculture, disease-resistant breeding and chemical agents are mainly used to gradually control rice blast. However, due to the complex genetic background of Ma...

AI Technical Summary

Problems solved by technology

However, due to the complex genetic background of the rice blast fungus, it is easy to mutate, and it is difficult for the disease-resistant varieties to keep up with the variation speed of the physiological races of the pathogenic bacteria, which often leads to the loss of resistance of a new disease-resistant rice variety after a few years of planting.

However, chemical control will not only pollute the environment, reduce the quality of rice, and destroy the ecology, but also lead to the continuous increase of drug resistance of bacteria and reduce the control effect.