Downy mildew bacterium culture and observation method

Abstract

The invention relates to the technical field of biology, in particular to a downy mildew bacterium culture and observation method. The method comprises the following steps: collecting downy mildew bacteria; preparing sporangium suspension liquid; and culturing the sporangium suspensions. Through repeated tests, a technology capable of inducing sporangium of cucumber downy mildew to germinate andrelease zoospore is obtained, the zoospore forms static spores through movement and morphological changes, the static spores germinate to form appressorium, and the appressorium germinates to generateinfection filaments. The technology can provide an effective technique for researchers to deeply research downy mildew biological basis, disease resistance identification, bactericide screening and the like. By means of the method, researchers can achieve quantitative statistics and monitoring of the number of zoospore of downy mildew, morphological changes and the number of formed infectors, andresearch of the action mechanism of the bactericide can also be achieved. Several development stages which must be experienced before downy mildew bacteria invade a host are clearly observed. The systematic observation method can provide first-hand data for prevention and treatment of the pathogenic bacteria on symptomatic drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to methods for cultivating and observing downy mildew bacteria. Background technique [0002] Cucumber downy mildew is an important disease in cucumber production, and its spreading speed is very fast. Lesions formed on leaves often lead to dry leaf tissue, which has a serious impact on cucumber production. A lot of research has been carried out at home and abroad on the prevalence of cucumber downy mildew in the field, its correlation with environmental temperature and humidity, and the establishment of early warning models. However, the research on the biological basis of cucumber downy mildew (Pseudoperonospora cubensis) is relatively weak. The important reason is the lack of relevant observation and experiment techniques, which restricts the research on the biological basis of downy mildew. Cucumber downy mildew as an oomycete cannot be cultivated artificially, which limits the res...

AI Technical Summary

Problems solved by technology

The zoospores released by the sporangia of downy mildew and the statistics of the number of zoospores cannot be counted. There are no detailed reports on the five key developmental stages before invading the host, such as swimming, forming stationary spores, and germination to form appresses.

Furthermore, the observation of the activity process before the downy mildew invades the host is limited, resulting in slow progress of related research

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