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Medicine for preventing and controlling magnaporthe grisea

A technology for rice blast fungus and rice blast disease, which is applied in the field of drugs for the prevention and control of rice blast fungus, can solve problems such as pesticide residues, resistance loss, and drug resistance of pathogenic bacteria, and achieve good application value.

Active Publication Date: 2021-04-06
SOUTH CHINA AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these strategies have their own limitations: the planting of a single disease-resistant variety in a large area in successive years leads to the loss of resistance of the variety; the application of pesticides is effective in disease control, but it is easy to pollute the environment, cause pesticide residues, and cause adverse consequences such as pathogenic bacteria resistance. ;Biological control is a very effective measure to prevent and control rice blast, but it is time-consuming and labor-intensive to find and develop bio-control bacteria, and the efficacy of bio-control bacteria is prone to instability

Method used

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  • Medicine for preventing and controlling magnaporthe grisea
  • Medicine for preventing and controlling magnaporthe grisea
  • Medicine for preventing and controlling magnaporthe grisea

Examples

Experimental program
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Embodiment 1

[0025] 1 Materials and methods

[0026] 1.1 Test strains

[0027] Field strain B157 of Magnaporthe grisea was donated by Naweed I.Naqvi Laboratory of Temasek Institute of Life Sciences, Singapore; Agrobacterium strain AG1 was preserved by our laboratory.

[0028] 1.2 Test plants

[0029] The susceptible rice LTH was donated by Dr. Jiang Liqun from Guangdong Rice Institute.

[0030] 4. Tangeretin (Sigama, T8951).

[0031] 1.3 Main instruments

[0032] Optical microscope (OLYMPUS)

[0033] 1.4 The formulation of the culture medium

[0034] 1) Prune-agar (PA) (1L) medium for culture and sporulation of Magnaporthe grisea: 40ml Prune fruit juice, 2.5g lactose, 2.5g sucrose, 1g yeast extract, 20g agar powder, adjust the pH to 6.5 with 10M NaOH.

[0035] 1.5 Detection of susceptibility of conidia of Magnaporthe grisea to hesperetin

[0036] Collect the conidia of the PA plate culture strain and adjust the concentration to 10 5 pcs / ml, 200 μM tangeretin to test the sensitivity...

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Abstract

The invention discloses a medicine for preventing and controlling magnaporthe oryzae. According to the invention, research results find that hesperetin can interfere with conidia to form appressorium, has a remarkable delaying effect on the formation of magnaporthe oryzae appressorium, has an obvious inhibiting effect on magnaporthe oryzae and inhibits the pathogenicity of magnaporthe oryzae, so that the hesperetin has good application value in the aspects of magnaporthe oryzae prevention and control and drug development.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, more specifically, to a medicine for preventing and controlling blast fungus. Background technique [0002] Rice is one of the three major grains in the world and is a very important food crop. Rice blast is a rice disease caused by Magnaporthe oryzae. It is the first of the three major diseases of rice. It occurs in all rice-growing regions in the world. It has caused large-scale losses in production and has become a major threat to food security. Moreover, rice blast often recurs and is extremely difficult to control. [0003] The current main control strategies for rice blast include breeding of resistant varieties, application of chemical pesticides and biocontrol bacteria to prevent and control rice blast fungus (Sun Shuyuan et al., 1986; Law et al., 2017; Xing et al., 2017; Zhu et al. al., 2016). However, these strategies have their own limitations: the planting of a s...

Claims

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Application Information

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IPC IPC(8): A01N43/42A01P3/00
CPCA01N43/42
Inventor 张炼辉邓懿祯梁美玲叶慧娟谷文祥
Owner SOUTH CHINA AGRI UNIV
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