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A bacteriostatic Burkholderia forest mp5 strain and its application

A Burkholderia and bacteriostatic technology, applied in the field of Burkholderia forest MP5 strain, can solve problems such as affecting the sustainable and healthy development of agriculture, destroying the ecological balance, and endangering the health of consumers. , to achieve the effect of good market application prospects

Active Publication Date: 2020-05-26
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Botrytis cinerea reproduces very fast and produces a large number of conidia, so it is easy to mutate and produce drug resistance. At present, it has been used in agricultural production for benzimazoles, dicarboximides, and carbamates. Resistance to various fungicides such as fungicides, which not only leads to the reduction of control efficiency, but also leads to the continuous increase of the amount of pesticides used
Excessive use of chemical agents not only causes excessive pesticide residues in agricultural products, endangers the health of consumers, but also causes environmental pollution, destroys ecological balance, and affects the sustainable and healthy development of agriculture.

Method used

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  • A bacteriostatic Burkholderia forest mp5 strain and its application
  • A bacteriostatic Burkholderia forest mp5 strain and its application
  • A bacteriostatic Burkholderia forest mp5 strain and its application

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Embodiment 1

[0024] 1. Isolation and screening of strains

[0025] On June 20, 2017, 20 tomato root samples were collected in Laoshan, Qingdao. The samples were transferred to a sterilized mortar for grinding, diluted with sterile water to 10ml, and then diluted to 10ml with sterile water. -4 -10 -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 28°C.

[0026] Pick a single colony, and inoculate a single bacterial colony in YEPD (yeast extract 3g / l, peptone 10g / l, glucose 20g / l) liquid medium for shaking at 25°C-28°C for 96h, then 4°C at 9000rpm After centrifugation, the supernatant was filtered through a filter with a diameter of 0.22um for later use. According to different proportions, the filtrate was added to the YPD medium, and the conidia of Botrytis cinerea and Fusarium oxysporum were inoculated for static culture, and the spores were inoculated into YEPD as a control. When the germination ra...

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Abstract

The present invention discloses a strain of Burkholderia forestae MP5 with antibacterial effect and its application. The present invention obtains strains capable of inhibiting plant wilt caused by Botrytis cinerea and Fusarium oxysporum through screening. MP5 strain, its specific taxonomic designation is Burkholderia arboris MP5, which was deposited in the China Center for Type Culture Collection, and its preservation number is CCTCC NO: M 2019702. The present invention proves that the MP5 bacterial strain obtained by the present invention has a strong inhibitory effect on Botrytis cinerea and Fusarium oxysporum through experiments on Botrytis cinerea and Fusarium oxysporum spores and germ tube elongation, and it can be prepared as a control The biocontrol agent of Botrytis cinerea and Fusarium wilt has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Burkholderia forestae MP5 strain with bacteriostasis and application thereof. Background technique [0002] Botrytis cinerea is an important plant pathogenic fungus, which is widely distributed in the world and is known as the second most destructive plant pathogen. This fungus can infect more than 500 important economic crops such as tomato, cucumber, grape and strawberry , can cause fruit and vegetable gray mold. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to wide sources of infection and rapid spread of the fungus. Botrytis cinerea is becoming more and more serious, and its occurrence and prevalence often cause serious economic losses. According to reports,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/205C12R2001/01
Inventor 梁文星杨震宙宋丽敏王光远
Owner QINGDAO AGRI UNIV
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