154 results about "Botryotinia polyblastis" patented technology

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention discloses an antibiotic Bacillus amyloliquefaciens with powerful bacteriostasis and wide bactericidal spectrum. The strain has been preserved in the China General Microbiological Culture Collection Center and the taxonomy name of the strain is: Bacillus amyloliquefaciens with preservation number CGMCCNo.4777. The antibiotic Bacillus amyloliquefaciens has powerful bacteriostasis, wide bactericidal spectrum, high inhibition rate up to above 93.5% on the rice blast fungus, the Botrytis cinerea and the Phytophthora parasitica; the Bacillus amyloliquefaciens also has very strong stress resistance, and very strong resistance to the acid and alkali, and is suitable to be used as microbial bactericide.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention discloses a manufacturing method and the application for a wood fungus conidium dust agent. It gains wood fungus from separating leaf of vegetables, purifying and transplanting to PDA test tube bevel to be used as mother fungus, adjusting temperature, pH value, and oxygen supplying quantity to produce spore liquid, and gaining large quantity of dry proofing conidium by adopting solid fermenting, mixing crashed condium and superfine diatomite to form wood fungus conidium dust agent. The method could be used to prevent vegetable producing.

The invention provides bacillus subtilis having a preservation number of CGMCC No.11750, a microbial agent containing the bacillus subtilis and application of the bacillus subtilis or microbial agent in prevention and control of grey mold of tomatoes, cucumbers, strawberries and other fruits and vegetables. The bacillus subtilis having the preservation number of CGMCC No.11750 and the microbial agent containing the bacillus subtilis have a strong antagonizing effect on botrytis cinerea, have quick response and have long prevention lasting period. A corresponding microbial preparation can be prepared by utilizing the screened bacillus subtilis strains, and occurrence of grey mold on tomatoes, cucumbers, strawberries and other vegetables can be prevented and controlled by the bacillus subtilis after the prepared microbial agent is applied to the field, so that chemical pesticide application in the fruit and vegetable production process can be reduced.

The invention relates to biocontrol Bacillus velezensis SF259 and application thereof. The Bacillus velezensis strain is collected in the China Center for Type Culture Collection (CCTCC) on September20, 2019, with the collection name of Bacillus velezensis SF259 and the collection number of CCTCCM 2019735. The Bacillus velezensis has a significant inhibition effect on xanthomonas oryzae pv. oryzae (Xoo), also shows broad-spectrum bacteriostatic activity for xanthomonas oryzae pv. oryzicola (Xoc), X. campestris pv. musacearum, X. axonopodis pv. vignicola, X. campestris pv. malvacearum, botrytis cinerea and magnaporthe oryzae, and has important biocontrol application prospects.

The invention belongs to the field of food preservation, and particularly relates to a fresh-keeping method for prolonging preservation duration of strawberries. The method comprises the following steps: selecting materials, preparing strawberry extracts, preparing a mixed chitosan solution, preparing fresh-keeping liquid, and soaking and air drying strawberries. The main components of the fresh-keeping liquid are supernate extracted from the strawberries and chitosan, raw materials of the supernate and the chitosan can be obtained conveniently, and particularly the supernate of the strawberries can be directly extracted from the bought strawberries, and is not required to be separately bought. According to the method, bacillus subtilis is added into the fresh-keeping liquid, so that growth and reproduction of mycete and botrytis cinerea can be effectively inhibited. Active substances, such as subtilin and gramicidin are produced during the growth of bacillus subtilis cells, and have obvious inhibition effects on the mycete and botrytis cinerea; in addition, the bacillus subtilis quickly consumes oxygen in the environment, and produces organic acids, such as lactic acid, so that the PH value of the environment is reduced, the growth of the mycete and botrytis cinerea is indirectly inhibited, and the storage duration is greatly prolonged.

The present invention relates to a strawberry tree twig and leaf extract germicide and its production method. Said production method includes the following procedures: drying and pulverizing, reflux dissolving, centrifugal separation and vacuum concentration to obtain extract, then using (by weight portion) 30-80 weight portions of above-mentioned extract, 20-50 portions of solvent and 10-20 portions of adjuvant to prepare germicide emulsifiable concentrate or using 20-40 portions of the above-mentioned extract, 10-20 portions of solvent, 10-20 portions of adjuvant and 30-50 portions of deionized water to prepare them into germicide microemulsion. Said germicide can be mainly used for controlling cucumber anthrax bacteria, apple putrescence disease-producing germ and banded sclerotial blight of rice plant, etc.

Methods and compositions comprising essential oils or components thereof for protecting harvested fruits against decay caused by a specific pathogenic fungus are provided, such as Cinnamon cassia oil for protection of persimmons against Alternaria alternata, strawberries against Botrytis cinerea, or mandarins against Penicillium italicum or Penicillium digitatum.

The invention discloses a novel harmaline amide compound, a preparation method of the compound and an application of the compound in preparation of pesticides. The structural formula of the harmaline amide compound is shown as a formula (I); the structural formula is as shown in the specification, wherein in the formula (I), R1 is selected from hydrogen, methyl, phenyl, 2,4,5-trimethoxyphenyl, p-methoxyphenyl, p-trifluoromethylphenyl or p-chlorophenyl; R2 is selected from hydrogen or bromine; R3 is selected from hydrogen or ethyl; and R4 is selected from ethyl, 2-pyridyl, o-chlorophenyl, 4,6-dimethylpyrimidyl or p-trifluoromethylphenyl. The compound has good inhibitory activities on rhizoctonia solani, collectotrichum musae, grey mould germ of strawberry, fusarium oxyspirum F sp niveum and alternaria solani and has good application prospects in control of important agricultural pests. In addition, the artificial synthesis process is simple, the product purity is high, and the compound is suitable for large-scale industrial application.

The invention discloses a loop-mediated isothermal amplification primer composition for detecting benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The LAMP primer composition consists of the following four primers: an F3, a B3, an FIP and a BIP. The primer composition consists of primer groups designed by mismatching a basic group in a mutation region containing a 198 locus of a botrytis cinerea Pers. beta-tubulin gene; loop-mediated isothermal amplification reaction is used for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The invention further provides a loop-mediated isothermal amplification method for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers. and a kit used thereby.

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention discloses a separation method of botrytis cinerea. According to the method, single conidia are directly separated from a botrytis cinerea standard sample by adopting a fine, soft and elastic spore dragging filament and are cultured to form a botrytis cinerea strain with single genetic composition. The implementation and operation steps of the isolated culture are as follows: (1) collecting a botrytis cinerea standard sample; (2) firing spore dragging and dispersing filaments; (3) preparing a spore dragging and dispersing plate; (4) dragging and dispersing conidia; (5) cutting small square blocks of single spores; and (6) forming a monospore isolated strain. The method has the following advantages: (1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; (2) single spore strains can be separated and obtained at one time; (3) the separation work consumes less time and labor; and (4) the working efficiency of botrytis cinerea separation and the reliability of the separation result can be obviously improved.

The present invention provides a strain of Debaryomyces hansenii, wherein the strain is Debaryomyces hansenii XBL01 and has the preservation number of CGMCC No.10464. The present invention further provides applications of the strain and corresponding bacterial agents in preparation of pathogenic bacteria inhibitors, disease inhibitors, bio-pesticides and fruit fresh-keeping agents. According to the present invention, the Debaryomyces hansenii XBL01 CGMCC No.10464 strain can be well used for prevention and control of pathogenic bacteria, especially for prevention and control of Botrytis cinerea, can be used for preparation of fruit fungicides or fresh-keeping agents, does not have side effects, and can not cause great pressure on the environment and the ecology.

The invention relates to a 1,3,4-oxadiazole thioether compound as well as a preparation method and application thereof. The compound has a structure shown as a general formula (I) in the description.The compound has a good inhibition effect on pathogenic bacteria and fungi. By aiming at xanthomonas oryzae pv.oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjac xanthomonas oryzae, xanthomonas axonopodis pv.citri, botryosphaeria dothidea, bacterial canker and wilt of tomato, pseudomonas syringae pv.actinidae, Leucostoma cincta(Fr.:Fr.)Hohn, B.cinerea, F.oxysporum, S.sclerotiorum,G.zeae, P.infestans, P.cinnamomi and the like, good inhibition effects are achieved.

The invention discloses Saccharomyces cerevisiae BY23, with broad bacteriostasis spectrum and stable effect, for controlling postharvest diseases of fruits and a use method and use thereof. The strainhas a collection strain serial number of CGMCC No. 14905 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the saccharomyces cerevisiae comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell / mL bacterial suspension from thalli and sterile water; placing the fruits such as apples, pears, grapes or oranges into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits, and drying the fruits in the air; and placing the fruits into a fresh-keeping box, and carrying out storage at normal temperature. The saccharomyces cerevisiae strain can be used for simultaneously controlling blue molds, Botrytis cinerea and black spot of apples, Botrytis cinerea of pears, black spot, Fusarium molds, anthrax and Phoma glomerata of grapes and blue molds of oranges, and loss caused by the postharvest diseases is reduced, sothat the saccharomyces cerevisiae strain has a very good application prospect.

The present invention relates to a composition for preventing at least one plant disease resulted from infection of plant pathogens and a method for preparing the same. Particularly, the composition contains extract of at least one plant selected from the group consisting of Coptis chinensis Franch., Phellodendron genus plant, Sanguinaria canadensis and Chelidonium majus var. asiaticum which are natural plants having anti-plant pathogen activity or berberine which is purified therefrom. The composition of the present invention can be effectively used for preventing disease pathogens such as plant powdery mildew, anthracnose of pepper, damping-off of cucumber and gray mold rot of strawberry resulted from infection of plant pathogens such as Colletrotrichum gloeosporiodes, Pythium ultimum or Botrytis cinerea. For an alternative method for preparing the composition comprising berberine and / or derivatives thereof, the present invention provides various optimal culture condition and two phases cultivation methods using Phellodendron genus plant.

The invention relates to a novel rhizobiumleguminosarum strain, wherein the strain has high biological nitrogen fixation activity, has certain preventive and therapeutic effects on fungal diseases of plants and has good saline-alkaline resistance and high temperature resistance, so that the strain has functions of nitrogen fixation, disease prevention, stress resistance and yield increase. The rhizobiumleguminosarum is preserved in the China General Microbiological Culture Collection Center Committee on July 15, 2013 with a preservation number of CGMCCNo.7924. As shown in researches, the strain can nodulate on peanut roots and the strain has a good inhibitory effect on botrytis cinerea and pythium aphanidermatum and has saline-alkaline resistance and high temperature resistance.

The invention discloses a hanseniaspora vineae BY19 for preventing and controlling postharvest diseases of fruits and vegetables and with wide antibacterial spectrum and stable effect and a use methodthereof. The collection number of the strain in China General Microbiological Culture Collection Center is CGMCC No. 14903. The use method of the hanseniaspora vineae comprises the following steps of: activating the strain, carrying out fermentation culture by using YPD, centrifuging, and preparing thalli into 1*108 cell / mL of bacterial suspension by using sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citruses or cherry tomatoes and the like into the bacterial suspension, soaking for 30 seconds, then taking out, and carrying out air drying; puttinginto a fresh-keeping box, and storing at normal temperature. The hanseniaspora vineae strain disclosed by the invention has the beneficial effects that penicilliosis and botrytis cinerea of the apples, the penicilliosis and the botrytis cinerea of the pears, botrytis, aspergillosis, black spot, fusaridiosis and anthracnose of the grapes, the botrytis of the strawberries and the botrytis and the aspergillosis of the cherry tomatoes can be controlled simultaneously, so that the loss caused by the postharvest diseases is reduced and the application prospect is good.

The invention discloses banisterine benzoyl urea compounds and a preparation method and application thereof. The banisterine benzoyl urea compounds have a structural formula I shown in the specification; in the formula I, R1 can be methyl, phenyl, 3,4,5- triethoxy phenyl, p-methoxyphenyl or p-chlorphenyl, R2 can be H or Br, and X can be O or S. The banisterine benzoyl urea compounds have an outstanding insecticidal activity for Culex fatigans, prodenia litura and Chilo suppressalis; a part of the compounds are identical to a control insecticide Dibenzoyl-1-tert-butylhydrazine in term of activity; the banisterine benzoyl urea compounds have good inhibitory activity for rice sheath blight disease, alternaria solani, Collectotrichum musae, grey mould fruit rot of strawberry and sour rot pathogenic bacteria of citrus; a part of the compounds have better inhibitory activity for the above five phytopathogens than validamycin. The banisterine derivatives are simple in structure and easy to synthesize; the synthesis process is simple, the product purity is high; the banisterine benzoyl urea compounds and the preparation method thereof are suitable for large-scale industrial popularization and application.

The invention relates to irpex lacteus LL210, application thereof and a biocontrol microbial agent. The irpex lacteus provided by the invention is classified and named as Irpex lacteus, and the preservation number of the Irpex lacteus is CGMCC No.21057. A strain is separated from tomato leaves, and is non-toxic and free of pathogenicity. A plate confrontation test shows that the strain can effectively inhibit mycelial growth and spore germination of tomato botrytis cinerea and tomato late blight, and can be used for antagonizing plant pathogenic fungi tomato botrytis cinerea and / or phytophthora. The biocontrol microbial agent prepared from the irpex lacteus LL210 is used for preventing and treating tomato diseases, the prevention and treatment effects on tomato botrytis cinerea and late blight reach 81.35% and 87.68%, the biocontrol microbial agent has a good disease prevention effect and good compatibility with the environment, and a new microbial resource is provided for biological prevention and treatment of diseases of plants such as tomatoes.

The invention discloses a bactericidal composition containing bacillus amyloliquefaciens and difenoconazole, and application of the bactericidal composition. The bactericidal composition is prepared by taking the bacillus amyloliquefaciens and the difenoconazole as active components, wherein the mass ratio of the bacillus amyloliquefaciens to the difenoconazole is equal to (5 to1)-(1 to 5). The invention also discloses preparation methods of agricultural preparation forms, i.e., wettable powder and a suspending agent, of the bactericidal composition, and application of the agricultural preparation forms in control of strawberry anthracnose, powdery mildew and gray mold. Compared with a single agent, the bactericidal composition provided by the invention has an obvious synergistic effect for the strawberry anthracnose, the powdery mildew and the gray mold, and can remarkably improve prevention and control effects; the potential drug resistance risk of the strawberry anthracnose, the powdery mildew and the gray mold for the difenoconazole and the bacillus is reduced; after the bactericidal composition is used, the consumption of chemical pesticide is reduced, the pesticide cost is lowered, and the problems of environmental pollution and residue of agricultural products are effectively solved; the bactericidal composition is safe to humans and animals, good for organisms and environmentally safe, thus being widely applied to the control of the strawberry anthracnose, the powdery mildew and the gray mold.

The invention relates to a phenylimidazole derivative, a synthesis method of the phenylimidazole derivative and application of the phenylimidazole derivative to pesticide, particularly application tothe fungicide pesticide aspect, and belongs to the technical field of pesticide. In order to solve the technical problems, the invention provides a novel phenylimidazole derivative, a synthesis methodof the novel phenylimidazole derivative and application of the novel phenylimidazole derivative to the pesticide. The structure of the compound is shown as a formula I. The compound structure is simple and novel; the synthesis is easy; meanwhile, the fungicidal activity is realized; a better bacteriostasis or sterilization effect is achieved on important plant pathogenic fungi such as tomato early blight, tomato botrytis cinereal, cucumber fusarium oxysporum, magnaporthe grisea and rice rhizoctonia solani.

The invention relates to a preparation method for carbazolyl isopropanolamine derivative and application thereof. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwifruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteriaand the like.

The invention belongs to the field of chemical engineering and pesticides, and particularly relates to a heterocyclic compound containing bis(2-substituted sulfonyl-1,3,4-oxadiazole-5-radical) and application thereof. The structure of the heterocyclic compound is shown in the specification. In a general formula, R is hydrogen, C1-C5 alkyl radicals, C1-C5 halogenated alkyl radicals, C2-C5 alkenyl, and ester, benzyl or substituted benzyl of C2-C5, and Z is hydrocarbyl or substituted hydrocarbon thereof, phenyl, substituted phenyl, biphenylyl, substituted biphenylyl, naphthyl, substituted naphthyl, diphenyl hydrocarbyl, substituted diphenyl hydrocarbyl, and diphenyl ether or substituted diphenyl ether. The heterocyclic compound has very good inhibitory activity on rhizoctonia solani, fusarium asiaticum, borrytis cinerea, physalospora piricala, helminthossporium oryzae, tobacco bacterial wilt and rice brown blotches, can be applied to prevention and control of plant diseases and has wide application prospect.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention discloses a harmaline oxazoline compound and a preparation method and application thereof .The structural formula of the harmaline oxazoline compound is shown as the formula (I) in the description, wherein in the formula (I), R1 is methyl or phenyl or p-chlorophenyl or o-hydroxybenzyl, and R2 is o-hydroxybenzyl or 3-methoxy-4-hydroxyphenyl or 3,5-dimethyl-4-hydroxyphenyl or 2-furyl .The compound has significant cytotoxic activity on spodoptera frugiperda cultured cell lines Sf9, has good insecticidal activity on culex fatigans and lipaphis erysimi, has good growth and development regulating activity on prodenia litura, and also has good inhibitory activity on rhizoctonia solani, colletotrichum gloeosporioides, phytophoria melonis, fusarium oxysporum f .sp .niveum, pseudoperonospora sp, botrytis cinerea and fusarium oxysporum f.sp.cubense .Besides, the compound is simple in structure, easy to synthesize, simple in synthetic process, high in product purity and suitable for large-scale industrial application and popularization.

The invention relates to cloning of a Botrytis cinerea beta-tubulin gene and an application thereof in resistance monitoring of zoxamide, particularly discloses a molecular detection method for rapidly identifying insecticide resistance of Botrytis cinerea and a special primer, and belongs to the technical field of molecular organisms. The specific primer pair is composed of deoxyribonucleic acid (DNA) molecules shown in SEQ ID NO:1 (or SEQ ID NO:1 or SEQ ID NO:3) and SEQ ID NO:5. The point mutation method for detecting the insecticide resistance on the zoxamide generated by the Botrytis cinerea, which is provided by the invention, has the characteristics of being high in sensitivity, simple and rapid, and good in stability, can rapidly and sensitively detect the development trend of the resistance on the zoxamide caused by field botrytis cinerea, and timely adjusts the disease control strategy, so as to delay and control further development of the insecticide resistance.

The invention discloses a penicillium brefeldianum strain and application thereof. The penicillium brefeldianum strain is PFLJ04, the preservation name of the strain is penicillium brefeldianum, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation date is August 15, 2019; and the preservation number is CGMCC No. 18119. Penicillium is separated from paris polyphylla var. yunnanensis endophyte, and has a good inhibiting effect on panax notoginseng root rot fungi (fusarium oxysporum, fusarium solani and phytophthoracactorum), paris polyphylla leaf spot fungi and paris polyphylla botrytis cinerea (botrytis cinerea, phoma sp. and pestalotiopsis sp.) simultaneously.

The invention discloses a pichia cactophila strain BY35 for effectively controlling fruit postharvest diseases with advantages of wide antibacterial spectrum and stable effect and a using method of pichia cactophila. The strain has a strain collection number of CGMCC No. 14909 in China General Microbiological Culture Collection Center. The using method of the pichia cactophila strain comprises thefollowing steps: activating the strain, performing fermentation culture by YPD, centrifuging, and preparing the culture into bacterium suspension of 1*10<8>cells per mL with sterile water; adding apples, pears, grapes or citrus and the like into the bacterium suspension, soaking for 30 seconds, taking out the fruits, and airing; and putting into a preservation box, and storing at a normal temperature. The pichia cactophila is capable of simultaneously controlling apple penicillium disease and botrytis cinerea, penicilliosis and botrytis cinerea of pear fruits and fusarium wilt disease and anthracnose of grapes as well as blue mold of citrus fruits. Losses caused by the postharvest diseases are decreased, and the strain has excellent application prospects.