153 results about "Botryotinia polyblastis" patented technology

The invention belongs to the field of food preservation, and particularly relates to a fresh-keeping method for prolonging preservation duration of strawberries. The method comprises the following steps: selecting materials, preparing strawberry extracts, preparing a mixed chitosan solution, preparing fresh-keeping liquid, and soaking and air drying strawberries. The main components of the fresh-keeping liquid are supernate extracted from the strawberries and chitosan, raw materials of the supernate and the chitosan can be obtained conveniently, and particularly the supernate of the strawberries can be directly extracted from the bought strawberries, and is not required to be separately bought. According to the method, bacillus subtilis is added into the fresh-keeping liquid, so that growth and reproduction of mycete and botrytis cinerea can be effectively inhibited. Active substances, such as subtilin and gramicidin are produced during the growth of bacillus subtilis cells, and have obvious inhibition effects on the mycete and botrytis cinerea; in addition, the bacillus subtilis quickly consumes oxygen in the environment, and produces organic acids, such as lactic acid, so that the PH value of the environment is reduced, the growth of the mycete and botrytis cinerea is indirectly inhibited, and the storage duration is greatly prolonged.

The invention discloses a novel harmaline amide compound, a preparation method of the compound and an application of the compound in preparation of pesticides. The structural formula of the harmaline amide compound is shown as a formula (I); the structural formula is as shown in the specification, wherein in the formula (I), R1 is selected from hydrogen, methyl, phenyl, 2,4,5-trimethoxyphenyl, p-methoxyphenyl, p-trifluoromethylphenyl or p-chlorophenyl; R2 is selected from hydrogen or bromine; R3 is selected from hydrogen or ethyl; and R4 is selected from ethyl, 2-pyridyl, o-chlorophenyl, 4,6-dimethylpyrimidyl or p-trifluoromethylphenyl. The compound has good inhibitory activities on rhizoctonia solani, collectotrichum musae, grey mould germ of strawberry, fusarium oxyspirum F sp niveum and alternaria solani and has good application prospects in control of important agricultural pests. In addition, the artificial synthesis process is simple, the product purity is high, and the compound is suitable for large-scale industrial application.

The invention discloses a loop-mediated isothermal amplification primer composition for detecting benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The LAMP primer composition consists of the following four primers: an F3, a B3, an FIP and a BIP. The primer composition consists of primer groups designed by mismatching a basic group in a mutation region containing a 198 locus of a botrytis cinerea Pers. beta-tubulin gene; loop-mediated isothermal amplification reaction is used for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers.. The invention further provides a loop-mediated isothermal amplification method for detecting the benzimidazole fungicide E198A genotype-resistant botrytis cinerea Pers. and a kit used thereby.

The present invention relates to a composition for preventing at least one plant disease resulted from infection of plant pathogens and a method for preparing the same. Particularly, the composition contains extract of at least one plant selected from the group consisting of Coptis chinensis Franch., Phellodendron genus plant, Sanguinaria canadensis and Chelidonium majus var. asiaticum which are natural plants having anti-plant pathogen activity or berberine which is purified therefrom. The composition of the present invention can be effectively used for preventing disease pathogens such as plant powdery mildew, anthracnose of pepper, damping-off of cucumber and gray mold rot of strawberry resulted from infection of plant pathogens such as Colletrotrichum gloeosporiodes, Pythium ultimum or Botrytis cinerea. For an alternative method for preparing the composition comprising berberine and / or derivatives thereof, the present invention provides various optimal culture condition and two phases cultivation methods using Phellodendron genus plant.

The invention relates to a novel rhizobiumleguminosarum strain, wherein the strain has high biological nitrogen fixation activity, has certain preventive and therapeutic effects on fungal diseases of plants and has good saline-alkaline resistance and high temperature resistance, so that the strain has functions of nitrogen fixation, disease prevention, stress resistance and yield increase. The rhizobiumleguminosarum is preserved in the China General Microbiological Culture Collection Center Committee on July 15, 2013 with a preservation number of CGMCCNo.7924. As shown in researches, the strain can nodulate on peanut roots and the strain has a good inhibitory effect on botrytis cinerea and pythium aphanidermatum and has saline-alkaline resistance and high temperature resistance.

The invention discloses banisterine benzoyl urea compounds and a preparation method and application thereof. The banisterine benzoyl urea compounds have a structural formula I shown in the specification; in the formula I, R1 can be methyl, phenyl, 3,4,5- triethoxy phenyl, p-methoxyphenyl or p-chlorphenyl, R2 can be H or Br, and X can be O or S. The banisterine benzoyl urea compounds have an outstanding insecticidal activity for Culex fatigans, prodenia litura and Chilo suppressalis; a part of the compounds are identical to a control insecticide Dibenzoyl-1-tert-butylhydrazine in term of activity; the banisterine benzoyl urea compounds have good inhibitory activity for rice sheath blight disease, alternaria solani, Collectotrichum musae, grey mould fruit rot of strawberry and sour rot pathogenic bacteria of citrus; a part of the compounds have better inhibitory activity for the above five phytopathogens than validamycin. The banisterine derivatives are simple in structure and easy to synthesize; the synthesis process is simple, the product purity is high; the banisterine benzoyl urea compounds and the preparation method thereof are suitable for large-scale industrial popularization and application.

The invention discloses a bactericidal composition containing bacillus amyloliquefaciens and difenoconazole, and application of the bactericidal composition. The bactericidal composition is prepared by taking the bacillus amyloliquefaciens and the difenoconazole as active components, wherein the mass ratio of the bacillus amyloliquefaciens to the difenoconazole is equal to (5 to1)-(1 to 5). The invention also discloses preparation methods of agricultural preparation forms, i.e., wettable powder and a suspending agent, of the bactericidal composition, and application of the agricultural preparation forms in control of strawberry anthracnose, powdery mildew and gray mold. Compared with a single agent, the bactericidal composition provided by the invention has an obvious synergistic effect for the strawberry anthracnose, the powdery mildew and the gray mold, and can remarkably improve prevention and control effects; the potential drug resistance risk of the strawberry anthracnose, the powdery mildew and the gray mold for the difenoconazole and the bacillus is reduced; after the bactericidal composition is used, the consumption of chemical pesticide is reduced, the pesticide cost is lowered, and the problems of environmental pollution and residue of agricultural products are effectively solved; the bactericidal composition is safe to humans and animals, good for organisms and environmentally safe, thus being widely applied to the control of the strawberry anthracnose, the powdery mildew and the gray mold.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention relates to cloning of a Botrytis cinerea beta-tubulin gene and an application thereof in resistance monitoring of zoxamide, particularly discloses a molecular detection method for rapidly identifying insecticide resistance of Botrytis cinerea and a special primer, and belongs to the technical field of molecular organisms. The specific primer pair is composed of deoxyribonucleic acid (DNA) molecules shown in SEQ ID NO:1 (or SEQ ID NO:1 or SEQ ID NO:3) and SEQ ID NO:5. The point mutation method for detecting the insecticide resistance on the zoxamide generated by the Botrytis cinerea, which is provided by the invention, has the characteristics of being high in sensitivity, simple and rapid, and good in stability, can rapidly and sensitively detect the development trend of the resistance on the zoxamide caused by field botrytis cinerea, and timely adjusts the disease control strategy, so as to delay and control further development of the insecticide resistance.

The invention discloses a pichia cactophila strain BY35 for effectively controlling fruit postharvest diseases with advantages of wide antibacterial spectrum and stable effect and a using method of pichia cactophila. The strain has a strain collection number of CGMCC No. 14909 in China General Microbiological Culture Collection Center. The using method of the pichia cactophila strain comprises thefollowing steps: activating the strain, performing fermentation culture by YPD, centrifuging, and preparing the culture into bacterium suspension of 1*10<8>cells per mL with sterile water; adding apples, pears, grapes or citrus and the like into the bacterium suspension, soaking for 30 seconds, taking out the fruits, and airing; and putting into a preservation box, and storing at a normal temperature. The pichia cactophila is capable of simultaneously controlling apple penicillium disease and botrytis cinerea, penicilliosis and botrytis cinerea of pear fruits and fusarium wilt disease and anthracnose of grapes as well as blue mold of citrus fruits. Losses caused by the postharvest diseases are decreased, and the strain has excellent application prospects.

A N-hydrocarbyloxycarbonyl-2-thiazolethione derivative is prepared through condensation reaction between 2-thiazolethione and chlorocarbonate in organic solvent under existence of acid capture, and post-treating. It can be used as the insecticide for red spider and the bactericide for gray mold, powdery mildew, etc.