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Method for preparing Trichderma bacteria conididium powder and use thereof

A technology of conidia and Trichoderma, which is applied in the field of preparation of new biocontrol preparations, can solve the problems of adverse effects of diseases, increased humidity, etc., and achieve the effect of less frequency of application, high control effect and simple method

Inactive Publication Date: 2007-08-29
SHENYANG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of preparation method and the application thereof of the Trichoderma conidia biological control dust agent that is used for preventing and treating greenhouse vegetable leaf diseases (botrytis cinerea, leaf mold etc.), to overcome conventional spraying method to cause greenhouse Adverse effects of increased internal humidity on disease control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation process of Trichoderma conidia dust agent is as follows:

[0016] 1. Preparation of slant strains

[0017] Take 5 grams of tomato leaves, add 50 ml of sterile water, dilute with water to 1000 times after shaking; take 150 grams of potatoes, 20 grams of glucose, 0.07 grams of Bengal red, 0.03 grams of streptomycin sulfate, 17 grams of agar, water 1000 milliliters, made into mother culture medium. The production method is as follows: peel the potatoes and cut them into small pieces of 1 cm square, add 1000 ml of water to boil for 30 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it After completely melting, put it into a triangular flask for sterilization, and sterilize it at 121°C for 30 minutes, and add streptomycin sulfate before pouring the plate. Take the diluted solution and the parent strain medium in a weight ratio of 1:9 and pour it into a petri dish to make a medium...

Embodiment 2

[0026] 1. Preparation of slant strains

[0027] Take 8 grams of cucumber leaves, add 80 ml of sterile water, dilute with water to 8000 times after shaking; take 180 grams of potatoes, 18 grams of glucose, 0.05 grams of Bengal red, 0.04 grams of streptomycin sulfate, 18 grams of agar, water 1000 milliliters, made into mother culture medium. The production method is as follows: peel the potatoes and cut them into small pieces of 1 cm square, add 1000 ml of water to boil for 30 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it After completely melting, put it into a triangular flask for sterilization, and sterilize it at 121°C for 30 minutes, and add streptomycin sulfate before pouring the plate. Take the diluted solution and the mother strain culture medium in a weight ratio of 1:9 and pour it into a petri dish to make a culture medium plate, culture it at 26°C for 48 hours, obtain the Trichoderma s...

Embodiment 3

[0036] 1. Preparation of slant strains

[0037] Take 10 grams of tomato leaves, add 100 ml of sterile water, dilute with water to 10000 times after shaking, take 200 grams of potatoes, 15 grams of glucose, 0.04 grams of Bengal red, 0.05 grams of streptomycin sulfate, 20 grams of agar, 1000 milliliters of water is prepared as a culture medium for mother bacteria. The production method is as follows: peel the potatoes and cut them into small pieces of 1 cm square, add 1000 ml of water to boil for 30 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it After completely melting, put it into a triangular flask for sterilization, and sterilize it at 121°C for 30 minutes, and add streptomycin sulfate before pouring the plate. Take the diluted solution and the parent strain culture medium in a weight ratio of 1:9 and pour it into a petri dish to make a culture medium plate, culture it at 28°C for 48 hours, o...

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PUM

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Abstract

The invention discloses a manufacturing method and the application for a wood fungus conidium dust agent. It gains wood fungus from separating leaf of vegetables, purifying and transplanting to PDA test tube bevel to be used as mother fungus, adjusting temperature, pH value, and oxygen supplying quantity to produce spore liquid, and gaining large quantity of dry proofing conidium by adopting solid fermenting, mixing crashed condium and superfine diatomite to form wood fungus conidium dust agent. The method could be used to prevent vegetable producing.

Description

technical field [0001] The invention relates to a production method of a biocontrol fungus used for preventing and treating greenhouse leaf diseases—Trichoderma viride (Trichoderma viride) conidia dust agent, specifically a method for preparing a novel biocontrol agent of a hyperparasitic fungus and its application. Background technique [0002] With the rapid development of the planting area of ​​protected areas, vegetables with high economic value such as cucumbers, tomatoes, and peppers have been monocultured for many years, and diseases have become more and more serious, especially Botrytis cinerea, which often results in a 20-30% reduction in production. Not even harvested. The prevention and control of vegetable diseases in protected areas has always been dominated by chemical prevention. Frequent application of pesticides to control diseases is not only costly, labor-intensive and time-consuming, but also has high pesticide residues in vegetables, which is harmful to...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04C12R1/885
Inventor 庄敬华陈捷杨长成高增贵
Owner SHENYANG AGRI UNIV
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