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Identification method for zanthoxylum nitidum var. tomentosum

A kind of technology of rhodopsin and rhodopsin, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of unstable baseline, asymmetric peak shape, poor resolution, etc., achieve stable baseline, moderate retention time, improve The effect of separation

Active Publication Date: 2020-04-21
GUIZHOU JINGFENG INJECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technical scheme has certain effect, but when measuring due to HPLC: chromatographic column mobile phase is: acetonitrile-0.1% phosphoric acid (21: 79), but this method peak shape is asymmetrical, and baseline is unstable, easy to drift, and resolution is relatively poor

Method used

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  • Identification method for zanthoxylum nitidum var. tomentosum
  • Identification method for zanthoxylum nitidum var. tomentosum
  • Identification method for zanthoxylum nitidum var. tomentosum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Chromatographic conditions and system applicability: octadecylsilane-bonded silica gel was used as filler, methanol-acetonitrile-0.1% phosphoric acid solution (5:5:90) was used as mobile phase, detection wavelength was 230nm, flow rate: 1mL·min- 1. The number of theoretical plates should not be less than 3000 based on the needle peak on both sides of the hair.

[0036] Preparation of Reference Substance Solution Take an appropriate amount of Rhizoma Acupuncture Reference Substance, accurately weigh it, and add methanol to make a solution containing 30 μg per 1 ml.

[0037] The specific preparation method of the test solution is as follows: take golden chicken tablets, remove the coating, grind finely, and accurately weigh 2.5g; or take golden chicken pellets, grind finely, and accurately weigh 0.5g; or take golden chicken pellets, grind finely, and accurately weigh Weigh 4.0g; place a stoppered conical flask, precisely add 25ml ethyl acetate, weigh it, ultrasonically treat i...

Embodiment 2

[0041] Chromatographic conditions and system applicability: octadecylsilane-bonded silica gel is used as filler, methanol-acetonitrile-0.1% phosphoric acid solution (25:25:50) is used as mobile phase, detection wavelength is 328nm, flow rate: 1mL·min- 1. The number of theoretical plates should not be less than 3000 based on the needle peak on both sides of the hair.

[0042] Preparation of Reference Substance Solution Take an appropriate amount of Rhizoma Acupuncture Reference Substance, accurately weigh it, and add methanol to make a solution containing 30 μg per 1 ml.

[0043] Preparation of test solution: Take Jinji tablets, remove the coating, grind finely, and weigh accurately 2.5g; or take Jinjiwan, finely grind, and weigh precisely 0.5g; or take Jinji pellets, grind finely, and weigh accurately 4.0g ; Place a stoppered conical flask, accurately add 35ml ethyl acetate, weigh it, ultrasonic treatment (power 500W, frequency 40kHz) for 25 minutes, let it cool, weigh it, make up ...

Embodiment 3

[0047] Chromatographic conditions and system applicability: octadecylsilane-bonded silica gel was used as filler, methanol-acetonitrile-0.1% phosphoric acid solution (8:20:72) was used as mobile phase, detection wavelength was 255nm, flow rate: 1mL·min- 1. The number of theoretical plates should not be less than 3000 based on the needle peak on both sides of the hair.

[0048] Preparation of test solution: Take Jinji tablets, remove the coating, grind finely, and weigh accurately 2.5g; or take Jinjiwan, finely grind, and weigh precisely 0.5g; or take Jinji pellets, grind finely, and weigh accurately 4.0g ; Place a stoppered conical flask, accurately add 28ml ethyl acetate, weigh it, ultrasonic treatment (power 500W, frequency 40kHz) for 13 minutes, let it cool, weigh it, make up the lost weight with ethanol, shake it well, Filter, accurately measure 4ml of the subsequent filtrate, add it to a neutral alumina column, the column specification is 100-200 mesh, 2g, the inner diameter...

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Abstract

The invention relates to an identification method for zanthoxylum nitidum var. tomentosum. The identification method is mainly used for identifying the zanthoxylum nitidum var. tomentosum in Jinji tablets, Jinji pellets and Jinji particles, and is characterized in that the identification method is specifically used for detecting the zanthoxylum nitidum var. tomentosum by adopting a high performance liquid chromatography. The identification method comprises the following steps of setting chromatographic conditions and system applicability, preparing a reference solution, preparing a test solution, measuring the solutions, and carrying out result judgment. After adjusting a mobile phase and adopting a test sample treatment method, the identification method has the advantages that the peak number is large, peak shapes are symmetrical, the separation degree is good, the retention time of each peak is moderate, a base line is stable, drifting is not likely to happen, meanwhile, the separation degree of a chromatogram is improved, the trailing phenomenon of the chromatogram is avoided, the product quality is effectively controlled, and the clinical medication curative effect is improved.

Description

Technical field [0001] The invention relates to the field of drug detection, in particular to an identification method for hair lilac needles. Background technique [0002] Two-faced needles are aliased to the ground golden bull, red heart thorn diao root, red barb bob, two back needle, double-sided needle, double-sided thorn, leaf piercing needle, big leaf cat branch bob, Shangshan tiger, Xiashan tiger, Sansho, wood Liana; stems, branches, leaf shafts and both sides of leaflet midribs have hook-like prickles. Singular pinnate compound leaf, 7-15 cm long; leaflets 3-11, opposite, leathery, ovate to ovate oblong, glabrous, slightly shiny above, corymbose panicle, axillary; flowers 4 Count; sepals broadly ovate. Follicles are purplish red when they are mature, with thick glands, and a short beak at the top. They are generally thick slices or cylindrical short segments, 2-20cm long and 0.5-6(10)cm thick. Efficacy: Wind-cold and dampness arthralgia, muscle and bone pain, bruises, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/065
Inventor 杨一秦梅元王明慧
Owner GUIZHOU JINGFENG INJECTION
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