A method for extractING
genomic DNA of Lonicera
japonica Maxim belongs to field of
molecular biology. The method mainly comprises the following steps: firstly,
cell walls and
cell membrane are brokenso that
DNA is fully released into buff; secondly, the interference of
protein,
polysaccharide,
pigment and other impurities was eliminated; finally,
DNA degradation is prevented. The sample was treated with SET
nucleus separation buffer, which could separate the
nucleus from the secondary substances such as polysaccharides and polyphenols in the supernatant, PVP added in the
grinding process andbeta-Mercaptoethanol, can effectively prevent the oxidation of polyphenols,
chloroform /
isoamyl alcohol extraction, the addition of diluted CTAB to remove polysaccharides. The leaves of Caprifoliaceaeplants are rich in carbohydrates and polyphenols, These substances not only affect the extraction of
DNA, but also affect the activity of Tag
enzyme in PCR. By this method, DNA with high yield and good quality can be obtained. DNA electrophoretic bands are clear and bright, and there is no
tail-
trailing phenomenon, which can meet the further research at
molecular level.