The invention discloses a purification method of echinocandins
antifungal drug anidulafungi. The method comprises the following steps of: (1) preparing a dry sample: adding
organic solvent in a crude product of the anidulafungin to dissolve the crude product, adding
silica gel after the crude product of the anidulafungin is adequately dissolved, and uniformly mixing and
drying the mixture to obtain the anidulafungin dry sample; (2) pressurizing and eluting: uniformly filling the anidulafungin dry samples in the top end of a
chromatographic column with
silica gel, pressurizing and eluting the
chromatographic column with the anidulafungin dry samples by adding
elution solvent, utilizing a high-effective
liquid phase chromatography to monitor, and collecting the
elution solution with the anidulafungin content being greater than 98 percent; and (3) concentrating: concentrating the
elution solution with the anidulafungin content being greater than 98 percent until
dryness to obtain a pure product with the anidulafungin content being greater than 98 percent. By adopting the
column chromatography, simplicity in operation is realized, and the equipment cost is low; the
organic solvent with
low toxicity and low
boiling point is adopted as the elution solution, so that the subsequent recycling treatment is simple, and the
environmental pressure can be greatly reduced; and the purification
separation time is short, the purification effect is good, the purification yield is high, and the purification method is applicable to industrialized
mass production.