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Multivariate detection method based on quantum dot fluorescent nanospheres

A fluorescent nanometer and detection method technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measurement devices, etc., can solve the problems of poor anti-fluorescence bleaching ability, emission spectrum tailing, poor stability, etc., and avoid fluorescence resonance energy transfer. effect, the effect of broad application prospects

Active Publication Date: 2019-12-13
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the above-mentioned defects in the prior art, the technical problem to be solved by the present invention is how to solve the problems of poor stability of traditional organic fluorescent dyes, poor ability to resist fluorescent bleaching, and tailing of emission spectrum, so as to effectively avoid the ubiquitous existence of multi-color luminescent materials. Fluorescence resonance energy transfer effect

Method used

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  • Multivariate detection method based on quantum dot fluorescent nanospheres
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  • Multivariate detection method based on quantum dot fluorescent nanospheres

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Embodiment 1

[0033] The quantum dot fluorescent nanospheres prepared in this embodiment are CdSe / ZnS fluorescent nanospheres, wherein the quantum dots are CdSe / ZnS quantum dots with an emission wavelength of 525nm, and the polymer is styrene-maleic anhydride copolymer (PSMA). The method is the SPG film emulsification-emulsion solvent evaporation method, and the specific preparation steps are:

[0034] 50mg of CdSe / ZnS quantum dots with an emission wavelength of 525nm and 1g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 48mL of toluene to form a dispersed phase;

[0035] Dissolve 8g of sodium dodecyl sulfate (SDS) in 1600mL of ultrapure water to form a 0.5wt% SDS solution as the continuous phase;

[0036] Adjust the nitrogen pressure so that the dispersed phase is dispersed into the continuous phase through the SPG membrane with a pore size of 0.3 μm under a pressure of 120 kPa to form a microemulsion;

[0037] After stirring for 24 hours, the toluene was volatilized, the ...

Embodiment 2

[0042] The quantum dot fluorescent nanospheres prepared in this embodiment are CdSe / ZnS fluorescent nanospheres, wherein the quantum dots are CdSe / ZnS quantum dots with an emission wavelength of 570nm, and the polymer is styrene-maleic anhydride copolymer (PSMA). The method is the SPG film emulsification-emulsion solvent evaporation method, and the specific preparation steps are:

[0043] 50mg of CdSe / ZnS quantum dots with an emission wavelength of 570nm and 1g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 48mL of toluene to form a dispersed phase;

[0044] Dissolve 8g of sodium dodecyl sulfate (SDS) in 1600mL of ultrapure water to form a 0.5wt% SDS solution as the continuous phase;

[0045] Adjust the nitrogen pressure so that the dispersed phase is dispersed into the continuous phase through the SPG membrane with a pore size of 0.3 μm under a pressure of 120 kPa to form a microemulsion;

[0046] After stirring for 24 hours, the toluene was volatilized, the ...

Embodiment 3

[0050] The quantum dot fluorescent nanospheres prepared in this embodiment are CdSe / ZnS fluorescent nanospheres, wherein the quantum dots are CdSe / ZnS quantum dots with an emission wavelength of 620nm, and the polymer is styrene-maleic anhydride copolymer (PSMA). The method is the SPG film emulsification-emulsion solvent evaporation method, and the specific preparation steps are:

[0051] 50mg of CdSe / ZnS quantum dots with an emission wavelength of 620nm and 1g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 48mL of toluene to form a dispersed phase;

[0052] Dissolve 8g of sodium dodecyl sulfate (SDS) in 1600mL of ultrapure water to form a 0.5wt% SDS solution as the continuous phase;

[0053] Adjust the nitrogen pressure so that the dispersed phase is dispersed into the continuous phase through the SPG membrane with a pore size of 0.3 μm under a pressure of 120 kPa to form a microemulsion;

[0054] After stirring for 24 hours, the toluene was volatilized, the ...

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Abstract

The invention discloses a multivariate detection method based on quantum dot fluorescent nanospheres. The method comprises the following steps: respectively coating polymers with quantum dots with different emission wavelengths to prepare the quantum dot fluorescent nanospheres; using the quantum dot fluorescent nanospheres as fluorescent reporter molecules to mark a detection target object, and providing a quantitative detection signal; coating capture molecules corresponding to the detection target object on a polystyrene board; forming a sandwich structure, and performing multi-element quantitative detection through combining with the fluorescence immunoadsorption detection technology. According to the invention, the problems that the stability of traditional organic fluorescent dyes ispoor, the anti-fluorescence bleaching capability is poor and the transmission spectrum has a trailing phenomenon are solved; meanwhile, different quantum dots are separated from the space, so the fluorescence resonance energy transfer effect commonly existing between multi-color light-emitting materials is effectively avoided; multi-element quantitative detection of protein and nucleic acid is carried out in combination with a fluorescence immunoadsorption detection technology, so the method has very important significance and good application prospects in a biological multi-element detectiontechnology.

Description

technical field [0001] The invention relates to the technical field of preparation and application of micro-nano materials, in particular to a multiple detection method based on quantum dot fluorescent nanospheres. Background technique [0002] Current research on probe design mainly focuses on the detection of a single analyte, but many biological problems are related to the interaction of multiple factors, especially in the field of medical diagnosis, unit detection may lead to false positive results, so multiple detection is required to Make sure the diagnosis is correct. A key issue with multiplex assays is that several unique signals can be obtained in a single run. Electrical signals, optical signals, mass spectra, or particle size can all be used as differential signals for multivariate detection. [0003] Enzyme-linked immunosorbent assay (ELISA) is currently the most commonly used method for detecting tumor markers. It is simple to operate, but only one analyte ca...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/02C09K11/88
CPCC09K11/025C09K11/883G01N21/6428G01N21/6486
Inventor 李万万沈梦飞武卫杰
Owner SHANGHAI JIAO TONG UNIV
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