30 results about "Xanthomonas citri" patented technology

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention discloses Bacillus amyloliquefaciens F9 and application thereof in prevention and control of citrus bacterial canker. The strain F9 is preserved in China Center for Type Culture Collection in Wuhan University, China on September 26th, 2016 with the preservation number of CCTCC NO:M 2016519. Plate antagonistic experiments find that the strain F9 has citrus bacterial canker inhibitory activity, and an inhibition zone is 26.5mm; supernatant of fermentation broth of the strain F9 after 4 days has antibacterial activity, and the diameter of the inhibition zone can reach 26.5mm; and an in vitro inoculation experiment finds that the strain F9 can inhibit extension and colonization of xanthomonas citri. Therefore, the Bacillus amyloliquefaciens F9 can be applied to prevention and control of the citrus bacterial canker as a biocontrol bacterium.

The invention relates to a 1,3,4-oxadiazole (thiadiazole) imidazole compound as well as a preparation method and use thereof. The compound has a structure shown as a general formula (I) in the description. The compound has good inhibition effects on plant pathogenic bacteria, such as xanthomonas oryzae pv. oryzae and xanthomonas citri.

The invention relates to an isopropanolamine substructure-containing 1, 2, 3, 4-tetrahydro-beta-carboline compound and a preparation method and application thereof. The compound has a structure as shown in a general formula (I), based on 1, 2, 3, 4-tetrahydro-beta-carboline, a nitrogen-containing fragment is introduced into the system, a series of 1, 2, 3, 4-tetrahydro-beta-carboline compounds containing isopropanolamine substructures are synthesized, and the compounds have an excellent inhibition effect on plant pathogenic bacteria such as xanthomonas oryzae pv. oryzae, xanthomonas citri, actinidia canker and the like.

The invention discloses a myricetin derivative containing quinoxaline and a preparation method and application thereof. The myricetin derivative has a structural formula (A), wherein R is positioned at the 3rd, 5th, 6th, 7th or 8th site of the quinoxaline structure and contains more than one hydrogen atom, hydroxyl, C1-6 alkyl, C1-6 alkoxy and halogen atom; n is the number of carbons in the carbonchain, such as 3, 4, 5 and 6. The myricetin derivative has good preventing and controlling effects on tobacco mosaic viruses, xanthomonas citri and xanthomonas oryzae. The structural formula (A) is shown in the attached figure.

The invention relates to a preparation method for carbazolyl isopropanolamine derivative and application thereof. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwifruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteriaand the like.

Disclosed herein our novel methods of increasing resistance of Citrus plants to diseases, in particular, citrus canker disease caused by Xanthomonas citri ssp. citri and Huanglongbing disease. Some embodiments relate to novel methods of altering gene sequence, structure and expression of certain disease susceptibility genes in the Citrus plant. Other embodiments relate to gene constructs equipped to be introduced into Citrus cells and direct modifications to target gene sequences.

The invention relates to a 4-amino quinazoline compound as well as a preparation method and application thereof. The compound has a general formula I) as shown in the specification, a quinazoline compound is taken as a basis, an aromatic ring methylene is introduced into the system for synthesizing a series of aromatic ring-containing methylene substituted 4-amino quinazoline compounds, the compound has a good inhibiting effect on pathogenic bacteria of plants, and has a good inhibiting effect on pathogenic bacteria such as xanthomonas oryzae, Xanthomonas citri and the like.

The invention discloses a pyrazolyl-oxa(thia)diazole compound containing pyridinium salt, and a preparation method and application thereof. The compound has the structure as shown in the following general formula (I). On the basis of a pyrazolyl-oxa(thia)diazole thioether(oxoether) compound, a pyridinium group capable of improving the biological activity of a target compound is introduced into the system to synthesize a series of pyrazolyl-oxa(thia)diazole oxoether or thioether amphipathic molecules containing the pyridinium salt; moreover, the compound has a good inhibition effect on pathogenic bacteria and fungi, has good inhibition effects on bacteria (bacterial leaf blight of rice, ralstonia solanacearum, xanthomonas citri and the like) and fungi (gibberella zeae, phytophthora infestans, rhizoctonia solani, botrytis cinerea pers, fusarium oxysporum, apple rot pathogenic bacteria and the like), and provides an important scientific base for research, development and creation of novel pesticides.

The invention belongs to the field of pesticides, and discloses a quinazoline-containing 1,3,4-oxadiazole derivative, and a preparation method and application thereof. The structure of the derivativeis shown as in a specification (I). The compound disclosed by the invention has a simple preparation method, has an obvious inhibiting effect on xanthomonas citri and ralstonia solanacearum, and can be applied to preventing and treating plant pathogenic bacteria.

The invention relates to heterocyclic substituted 1, 3, 4-oxadiazole hydrazide compounds and a preparation method and application thereof. The compound has a structure shown as a general formula (I). The compound has a relatively good inhibition effect on pathogenic fungi and bacteria; and the compound has good inhibition effects onfusarium graminearum, potato late blight bacteria, blueberry root rot bacteria, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, botryosphaeria dothidea, rhizoctonia solani, xanthomonas oryzae pv., ralstonia solannacearum, Xanthomonas citri, kiwi canker bacteria, Xanthomonas oryzae cucumber, Xanthomonas oryza konjak, Botryosphaeria dothidea, tomato canker pathogens, apple canker pathogens and the like.

The invention relates to streptomyces lincolnensis capable of preventing and treating peach brown rot. The streptomyces lincolnensis is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 17, 2020, and has a preservation number of CGMCC No.21377. The invention also relates to application of the streptomyces lincolnensis in preventing and treating the peach brown rot. The streptomyces lincolnensis disclosed by the invention has a very strong inhibition effect on various plant pathogenic bacteria of the peach brown rot and the like on a flat plate, and volatile metabolites of the streptomyces lincolnensis have a very good prevention and control effect on the growth of the pathogenic bacteria of the peach brown rot. The strain has potential commercial development and application values in biological control practice of diseases. The strain also has broad-spectrum antagonistic activity, shows a relatively strong bacteriostatic effect on pathogenic bacteria such as xanthomonas citri, pseudomonas angulate holland and the like, and also has a very good bacteriostatic effect on pathogenic fungi such as tobacco botrytis cinerea, valsa mali, thielaviopsis basicola and the like.

The invention discloses a method for screening a marker-free deletion mutant of an Xanthomonas citri subsp.citri hfq gene. The method for screening the marker-free deletion mutant of the Xanthomonas citri subsp.citri hfq gene comprises the steps: (1) extracting Xanthomonas citri subsp.citri DNA; (2) constructing an hfq gene deleted recombinant suicide plasmid vector: A, respectively amplifying upstream and downstream flanking sequences of the Xanthomonas citri subsp.citri hfq gene by using primers of Hfq-up-F, Hfq-up-R, Hfq-down-F and Hfq-down-R; B, recycling a PCR fragment; C, connecting a recycled product with a vector pEASY-T1vector; D, converting a connection product into escherichia coli; E, extracting a positive cloning plasmid; F, carrying out double enzyme digestion on upstream / downstream T-vector recombinant plasmid and pK18mobSacB; and G, connecting enzyme digestion products and converting a connection liquid into DH5(alpha) escherichia coli; (3) converting the recombinant suicide plasmid vector into Xanthomonas citri subsp.citri; and (4) screening the mutant by using a sucrose medium method.

The invention discloses a quinoxaline-containing chalcone derivative and a preparation method and application thereof. The general formula of the quinoxaline-containing chalcone derivative is shown inthe description, wherein X represents 2-O or 4-O, and R1 refers to a phenyl group, a heterocyclic group and one or more substituted phenyl groups with halogen at the 2nd-6th positions, C1-C6 alkyl atthe 2nd-6th positions, C1-C6 alkoxy at the 2nd-6th positions, nitro at the 2nd-6th positions, amino at the 2nd-6th positions and trifluoromethyl at the 2nd-6th positions; R2 represents one or more hydrogen atoms, C1-C6 alkoxy groups, nitro groups, C1-C6 alkyl groups and trifluoromethyl groups or halogen atoms at the 5th position, 6th position and 7th position or 8th position in a quinoxaline structure. The derivative can inhibit xanthomonas oryzae, xanthomonas citri and ralstonia solanacearum, raw materials are easily obtained, the reaction condition is mild, the post-treatment is simple, andthe yield is high.

The invention discloses N-substituent group-N-(substituted phenylaminocarbonyl)-1-substituted sulfonylthioformylhydrazide derivatives as well as a preparation method and application thereof. The derivatives have a structural general formula (I) shown in the description, wherein n = 0, 1 or 2, and X = C, N, O or S; R1 is C1-4 alkyl or substituted benzyl; and R2 is a hydrogen atom, an o-position, anm-position, a p-position monosubstituted halogen atom, C1-4 alkyl, C1-2 alkoxy, or a polysubstituted halogen atom. The derivatives provided by the invention have excellent antibacterial activity against bacterial diseases such as xanthomonas oryzae, xanthomonas oryzicola and xanthomonas citri, the synthetic raw materials have low costs, and the synthetic method is simple and easy.

The invention discloses chalcone derivatives containing 1,2,4-triazine and a production method and application thereof. A general formula of the chalcone derivatives containing the 1,2,4-triazine is as shown in the description, wherein n=2,3,4, R is phenyl or substituted phenyl or substituted aromatic heterocycte, and R1 is phenyl or substituted phenyl or alkyl of C1-C6. The chalcone derivatives containing the 1,2,4-triazine have good activity of inhibiting xanthomonas oryzae, xanthomonas citri and tobacco ralstonia solanacearum, raw materials are easy to obtain, reaction conditions are mild,post-treatment is simple, and the yield is high.

The invention discloses a compound for preventing and treating plant pathogenic bacteria and application thereof. The compound is 4-allyl catechol, tests prove that the 4-allyl catechol has antibacterial activity, and when the test concentration of the compound is 1000 [mu] mol / L, the compound has a good antibacterial effect on xanthomonas campestris pv, xanthomonas oryzae pv. oryzicola, xanthomonas citri, bacterial fruit blotch of muskmelon, bacterial wilt of tomato, bacterial angular leaf spot of strawberry, bacterial angular leaf spot of mango, black rot of cabbage and soft rot of potato, and the inhibition rate is 90% or above; meanwhile, in-vivo test results show that the compound has a good inhibition effect on pathogenicity of potato soft rot fungi, and also has a good prevention and treatment effect on mango bacterial angular leaf spot bacteria and melon bacterial fruit blotch bacteria. The results show that the 4-allyl catechol has a broad-spectrum bactericidal effect on plantpathogenic bacteria. Therefore, a foundation is laid for further creating a novel pollution-free botanical pesticide by utilizing a unique plant, namely South China pepper, in China.

The invention discloses a 2,5-substituent-1,3,4-oxadiazole sulfone derivative as well as a preparation method and application thereof. The 2,5-substituent-1,3,4-oxadiazole sulfone derivative has a general formula shown in a formula (I), wherein R1 is 4-chlorine or 4-fluorine; and R2 is methyl, ethyl, propyl, n-butyl, n-pentyl, benzyl, 4-chlorobenzyl or 4-fluorobenzyl. The 2,5-substituent-1,3,4-oxadiazole sulfone derivative disclosed by the invention has good activity on rice bacterial leaf blight and xanthomonas citri and is simple in structure, simple in preparation technology and low in production cost. (The formula (I) is described in the specification).

The invention discloses a method for rapidly evaluating canker resistance of a citrus variety, which comprises the following steps: firstly, constructing enhanced yellow fluorescent protein labeled citrus xanthomonas, then injecting or spraying and inoculating a fluorescence labeled citrus xanthomonas suspension to the leaf backs of the citrus variety to be evaluated, culturing in a greenhouse, taking leaves, grinding, and screening to obtain the canker resistance of the citrus variety. And observing the grinding liquid under a fluorescence microscope, counting fluorescence spots, calculating the growth change of bacteria, and evaluating the canker resistance. Researches show that enhancement of yellow fluorescent protein (eYFP) does not affect growth and toxicity of Xanthomonas citri (Xcc), and eYFP labeled Xcc can be successfully used for quantifying bacterial concentration and evaluating canker resistance of citrus, is faster and more effective than a plate colony counting method, has consistency with wild Xanthomonas citri, and can be used for detecting canker resistance of citrus. The canker resistance of the citrus variety can be rapidly evaluated.

The invention discloses a novel application of an acylated phloroglucinol derivative in prevention and treatment of fruit postharvest rot-causing fungi, agricultural plant pathogenic fungi, agricultural bacteria and food-borne pathogenic bacteria, and belongs to the field of bactericides. The twenty acylated phloroglucinol derivatives provided by the invention have the advantages that the twenty acylated phloroglucinol derivatives are used for treating postharvest rot-causing fungi such as botrytis cinerea, penicillium expansum ACCC30898, rhizopus stolonifer CICC 40327, monilinia coerulea ACCC 36263, aspergillus flavus ACCC32601 and the like; various plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, magnaporthe oryzae and phytophthora capsici; agricultural bacteria such as Xanthomonas oryzae pv. Oryzae ACCC 116602, Xanthomonas citri pv. Citri and the like; the compound has obvious bacteriostatic or bactericidal activity on food-borne bacteria such as bacillus cereus BNCC 103930, listeria monocytogenes ATCC 19115, salmonella enteritidis ATCC 14028, escherichia coli O157: H7 ATCC 35150 and the like, and can be developed as a broad-spectrum bactericidal lead compound applied to the agriculture or food industry.

The invention discloses a method for screening a marker-free deletion mutant of an Xanthomonas citri subsp.citri hfq gene. The method for screening the marker-free deletion mutant of the Xanthomonas citri subsp.citri hfq gene comprises the steps: (1) extracting Xanthomonas citri subsp.citri DNA; (2) constructing an hfq gene deleted recombinant suicide plasmid vector: A, respectively amplifying upstream and downstream flanking sequences of the Xanthomonas citri subsp.citri hfq gene by using primers of Hfq-up-F, Hfq-up-R, Hfq-down-F and Hfq-down-R; B, recycling a PCR fragment; C, connecting a recycled product with a vector pEASY-T1vector; D, converting a connection product into escherichia coli; E, extracting a positive cloning plasmid; F, carrying out double enzyme digestion on upstream / downstream T-vector recombinant plasmid and pK18mobSacB; and G, connecting enzyme digestion products and converting a connection liquid into DH5(alpha) escherichia coli; (3) converting the recombinant suicide plasmid vector into Xanthomonas citri subsp.citri; and (4) screening the mutant by using a sucrose medium method.

The invention discloses a compound which has bacteriostasis and bactericidal activity to xanthomonas citri. The minimal inhibitory concentration (MIC) value of the xanthomonas citri is 8 micrograms / mL, and the minimal bactericidal concentration (MBC) value of the xanthomonas citri is 32 micrograms / mL.

The invention relates to carbazolyl isopropanolamine derivatives with double chiral centers as well as a preparation method and application of the same. The compound has a structure as shown in a general formula (I), a series of carbazolyl isopropanolamine derivatives with double chiral centers are synthesized on the basis of carbazole, and the compound has an excellent inhibition effect on plant pathogenic bacteria such as xanthomonas oryzae pv. Oryzae, xanthomonas citri, xanthomonas axonopodis pv. Actinidia and the like.

The invention discloses a compound containing oxadiazole thioether and sulfone, a stereoisomer thereof, a salt thereof or a solvate thereof, a preparation method, a composition and application, the general formula structure of the compound is as follows: tetrahydropyran-4-formic acid is used as a basis, a heterocyclic compound is introduced into the system, a series of compounds containing oxadiazole thioether and sulfone are synthesized, and the compound containing oxadiazole thioether and sulfone can be used for preparing the compound containing oxadiazole thioether and sulfone. The compound has an excellent inhibition effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri.

The invention discloses a preparation method and application of a thiochroman-4-ketone derivative containing a 1, 3, 4-oxadiazole thioether and oxime ether structure, and the thiochroman-4-ketone derivative containing the 1, 3, 4-oxadiazole thioether and oxime ether structure has a general formula shown in the specification. According to the invention, a series of thiochroman-4-one derivatives containing 1, 3, 4-oxadiazole thioether and oxime ether structures are synthesized by taking substituted thiophenol as a raw material, and the in-vitro biological activity on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri is determined by adopting a turbidimetric method; the biological activity determination result shows that a part of target compounds have relatively good in-vitro biological activity on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, wherein the compound6-chlorine-2-(5-methylthio-1,3,4-oxadiazole-2-base)-4-methyl oxime ether based thiochroman-4-one (7a) has a better in-vitro biological activity to Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, and the inhibition medium concentration (EC50 value) is 17 mu g / mL and 28 mu g / mL respectively, which is superior to that of a control agent bismerthiazol and that of a control agent thiediazole copper.

The invention discloses a pyrazolyl-oxa(thia)diazole compound containing pyridinium salt, and a preparation method and application thereof. The compound has the structure as shown in the following general formula (I). On the basis of a pyrazolyl-oxa(thia)diazole thioether(oxoether) compound, a pyridinium group capable of improving the biological activity of a target compound is introduced into the system to synthesize a series of pyrazolyl-oxa(thia)diazole oxoether or thioether amphipathic molecules containing the pyridinium salt; moreover, the compound has a good inhibition effect on pathogenic bacteria and fungi, has good inhibition effects on bacteria (bacterial leaf blight of rice, ralstonia solanacearum, xanthomonas citri and the like) and fungi (gibberella zeae, phytophthora infestans, rhizoctonia solani, botrytis cinerea pers, fusarium oxysporum, apple rot pathogenic bacteria and the like), and provides an important scientific base for research, development and creation of novel pesticides.

The invention relates to a pentadienone compound containing triazine, and a preparation method and an application of the pentadienone compound. The compound is shown as formula A as shown in the specification. X selected from O; R is phenyl, substituted phenyl or substituted heterocyclyl; R1 is a hydrogen atom or methoxyl; and R2 is phenyl, substituted phenyl or C1-C6 alkyl. The compound has a better prevention and control effect on pseudomonas solanacearum, xanthomonas citri and xanthomonas campestris pv. oryzae.