The invention relates to a
phytophthora infestans culture medium and a preparation method thereof, and belongs to the field of ovum bacterium culture. The
phytophthora infestans culture medium comprises mixed liquid consisting of rye juice, tomato juice and
calcium carbonate, and
agar strips. The preparation method comprises specific preparation steps of weighing rye, adding an appropriate amountof water, performing sterilization in a high-pressure sterilization pot, adding water to the sterilized rye, performing crushing, performing boiling over, and performing
filtration so as to obtain therye juice;
cutting tomatoes into dices, performing crushing to obtain tomato original juice, and mixing the tomato original juice with
clean water in the volume ratio being 1 to 1 so as to obtain thetomato juice; adding the
calcium carbonate to the tomato juice in proportion, then mixing the rye juice with the tomato juice in proportion to obtain the mixed liquid, adding the
agar strips to the mixed liquid in proportion, adjusting pH value with a 0.1%
sodium hydroxide solution, and performing sterilization so as to complete the preparation of the culture medium. The culture medium disclosedby the invention can effectively promote growth of the
phytophthora infestans, the
spore production capacity of the
phytophthora infestans can be increased, the
spore production number is obviously increased, the preparation method is simple and the preparation time is short.