Phytophthora infestans culture medium and preparation method thereof

Abstract

The invention relates to a phytophthora infestans culture medium and a preparation method thereof, and belongs to the field of ovum bacterium culture. The phytophthora infestans culture medium comprises mixed liquid consisting of rye juice, tomato juice and calcium carbonate, and agar strips. The preparation method comprises specific preparation steps of weighing rye, adding an appropriate amountof water, performing sterilization in a high-pressure sterilization pot, adding water to the sterilized rye, performing crushing, performing boiling over, and performing filtration so as to obtain therye juice; cutting tomatoes into dices, performing crushing to obtain tomato original juice, and mixing the tomato original juice with clean water in the volume ratio being 1 to 1 so as to obtain thetomato juice; adding the calcium carbonate to the tomato juice in proportion, then mixing the rye juice with the tomato juice in proportion to obtain the mixed liquid, adding the agar strips to the mixed liquid in proportion, adjusting pH value with a 0.1% sodium hydroxide solution, and performing sterilization so as to complete the preparation of the culture medium. The culture medium disclosedby the invention can effectively promote growth of the phytophthora infestans, the spore production capacity of the phytophthora infestans can be increased, the spore production number is obviously increased, the preparation method is simple and the preparation time is short.

Description

technical field [0001] The invention belongs to the field of oomycete culture, and in particular relates to a culture medium for potato infestans and a preparation method thereof. Background technique [0002] Potato late blight is caused by Phytophthora infestans (Mont.) de Bary, an oomycete. It is one of the devastating diseases of potatoes. It can cause a 10% to 20% yield reduction in a normal year, and a 50% yield reduction in a severe year. % ~ 70%, or even no harvest. [0003] In order to control the disease of potato infestation, scientists have conducted a lot of research. The research shows that the sporangia produced by the strain of potato infestation is the key to the disease cycle of potato infestation. Therefore, more in-depth research should be carried out on this disease, and Phytophthora spores should be extracted Sac is one of the necessary jobs. However, the number of spores produced by artificially cultivating P. infestans in experiments is not ideal, e...

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Problems solved by technology

However, in the experiment, the number of spores produced by the artificial culture of P. infestans is not ideal, especially after long-term multi-generational cultivation, the number of sporangia produced by culture is difficult to meet the needs of the experiment, forming a bottleneck for research.

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