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Application of compound Quinolactacide

A compound and agricultural fungicide technology, applied in the application field of the compound quinolactacide, can solve problems such as environmental pollution and excessive pesticide residues, and achieve the effects of low toxicity to humans and animals, low product cost, and simple production process

Inactive Publication Date: 2017-07-21
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control methods mainly rely on chemically synthesized pesticides such as thiophanate-methyl and carbendazim, but long-term and large-scale use has brought about problems such as environmental pollution and excessive pesticide residues.

Method used

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  • Application of compound Quinolactacide
  • Application of compound Quinolactacide
  • Application of compound Quinolactacide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: the preparation of compound Quinolactacide

[0012] The preparation of the compound quinolactacide shown in formula (1) is referred to literature Masaki Abe, Tetsuya Imai, Naoki Ishii, et al. -1205.

Embodiment 2

[0013] Embodiment 2: antibacterial activity test

[0014] Adopt the microdilution method, measure the bacteriostatic activity of the compound shown in formula (1) to potato late blight (Phytophthorainfestans) (" from natural product to new pesticide creation-principle method ", Wu Wenjun, 2006, Chemical Industry Press).

[0015] 1) Preparation of bacterial suspension

[0016] Inoculate the fungus to be tested on the surface of PDA medium and culture at 28°C for 72 hours, draw 2 mL of sterile 0.85% NaCl solution (containing 0.25% Tween-20) to wash the culture, and gently scrape off the colony with a glass scraper. Draw an appropriate amount of bacterial suspension into a sterile test tube and adjust to 0.5 McFarland turbidity (equivalent to 1.5×10 8 CFU / mL) for standby;

[0017] 2) Sample preparation

[0018] Take 1 mg of the sample to be tested (compound of formula (1)), dissolve it in 100 μL DMSO, mix well, draw 50 μL of the sample solution into another centrifuge tube, an...

Embodiment 3

[0025] Embodiment 3: wettable powder

[0026] By fermenting and cultivating Penicillium citrinum Thom F 1539 (fermentation and culturing process can refer to the above-mentioned documents); the fermented liquid is subjected to pressure filtration and precipitation, and the supernatant is concentrated so that the content of the compound shown in formula (1) is 1.5-2.0 wt%, based on the mass of the concentrated supernatant, add 25-35% white carbon black for adsorption, then add 3-8% sodium lauryl sulfate, 0.8-1.2% CMC and 2-4% pull-off powder, Then add attapulgite to prepare a wettable powder with a content of 0.5 wt% of the compound represented by formula (1).

[0027] How to use: Spray 500 times liquid on potato stems and leaves.

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Abstract

The invention discloses the application of a compound Quinolactacide. The structure of the compound is shown in the formula (1); the compound has better inhibitory activity to the phytophthora infestans, and minimal inhibitory concentration (MIC) of 8mu g / mL can be used as a novel pesticide component or a lead compound with a bactericidal effect. The compound is a microorganism-derived natural product. Compared with a traditional chemical synthetic bactericide, the compound has the advantages of good environment compatibility and easiness in mass production.

Description

technical field [0001] The present invention relates to the use of microbial source fungicides, in particular the compound Quinolactacide. Background technique [0002] Potato late blight, also known as blight and potato plague, is a devastating fungal disease that causes the death of potato stems and leaves and tuber rot. When the disease is severe, the leaves of the plant will shrivel and shrink, and eventually lead to black rot of the whole plant, resulting in a serious reduction in potato production. Not even harvested. Potato late blight has occurred in major potato producing areas in my country, causing serious losses, usually 30% to 50% in a year, and even resulting in crop failure in a pandemic year. At present, the prevention and control methods mainly rely on chemically synthesized pesticides such as thiophanate-methyl and carbendazim, but long-term and large-scale use has brought about problems such as environmental pollution and excessive pesticide residues. Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 李光李洪文侯波
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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