The invention belongs to the field of biological feed, and relates to a preparation method of recombinant
manganese peroxidase and application of the recombinant
manganese peroxidase in degradation ofChinese herbal
medicine lignin. The method comprises the following steps: firstly, carrying out preference optimization on a
gene sequence of
manganese peroxidase coming from
Irpex lacteus, carryingout PCR amplification by taking the optimized
gene as a template, connecting the optimized
gene with pREP, then converting the connected gene into
escherichia coli competent cells to obtain recombinant shuttle plasmids, and introducing the recombinant shuttle plasmids into defective
schizosaccharomyces pombe competent cells to obtain recombinant
manganese peroxidase engineering bacteria; and performing culture to obtain a crude
enzyme liquid, mixing the crude
enzyme liquid with Chinese herbal medicines, and then adding
calcium chloride, manganese
sulfate,
laccase,
glucose oxidase and a small molecular substance activator for a multi-
enzyme synergistic enzymolysis reaction. According to the method provided by the invention, a large amount of
manganese peroxidase can be rapidly obtained, industrial production of the
manganese peroxidase is realized, a good and stable peroxidase product is prepared, and effective degradation of Chinese herbal
medicine lignin is realized.