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Albicans ll210 and its application, biocontrol agent

A technology of Raketooth albicans, LL210, applied in the direction of application, biocide, fungicide, etc., to achieve the effect of broad application prospects

Active Publication Date: 2022-06-14
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the effect of R. albicans on phytopathogenic fungi

Method used

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  • Albicans ll210 and its application, biocontrol agent
  • Albicans ll210 and its application, biocontrol agent
  • Albicans ll210 and its application, biocontrol agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and identification of antagonistic bacteria

[0024] The invention isolates and obtains a strain of R. albicans LL210 antagonizing tomato pathogenic fungi from tomato leaves, which is non-toxic and non-pathogenic, and provides new microbial resources for biological control of tomato and other plant diseases.

[0025] Select healthy leaves from the diseased tomato plants for sampling, put them into a sterilized ziplock bag prepared in advance, and bring them back to the laboratory for isolation. In the ultra-clean workbench, first put the material into sterile water for cleaning, then disinfect with 75% alcohol for 10-30s, rinse with sterile water for 3 times, then disinfect with mercuric chloride for 15 seconds, rinse with sterile water for 3 times, Blot excess moisture on paper. Use a scalpel to cut the material into small square pieces of about 1 cm, insert the wound into the PDA medium, and cultivate it in a 28°C constant temperature incubator fo...

Embodiment 2

[0032] Example 2 Inhibition of Mycelia Growth of the Tomato Botrytis Botrytis Pathogen

[0033] This example verifies the antagonistic effect of Irpex lacteus LL210 on a phytopathogenic fungus, which is Botrytis cinerea Pers.

[0034] On a PDA medium plate with a diameter of 9 cm, a 0.8-cm-diameter bacterium bacterium cake is placed on one side of the plate, and a bacterium cake of tomato gray mold pathogenic bacteria of the same size is placed on the other side, with an interval of 4.5 cm between the two bacterium cakes , placed in a constant temperature incubator at 28°C, and the antibacterial rates were counted on the 7th to 14th day.

[0035] The results are shown in the table below, the albicans has obvious inhibitory effect on the hyphal growth of Botrytis cinerea ( figure 2 ), the bacteriostatic rate when cultivating 7-14d is respectively:

[0036] time 7d 8d 9d 10d 11d 12d 13d 14d Antibacterial rate 64.4％ 66.7％ 73.3％ 73.3％ 81.1％ 86.7％ 8...

Embodiment 3

[0037] Example 3 Inhibition of Mycelia Growth of Tomato Late Blight Pathogenic Fungus LL210

[0038]This example verifies the antagonistic effect of Irpex lacteus LL210 on a plant pathogenic fungus, the said plant pathogenic fungus is Phytophthora tomato (P. infestans (Mont.) De Bary).

[0039] On the PDA culture medium plate with a diameter of 9 cm, the PDA bacterium bacterium cake with a diameter of 0.8 cm is placed on one side of the plate, and the tomato late blight pathogenic bacterium bacterium cake of the same size is placed on the other side, and the interval between the two bacterium cakes is 4.5 cm. They were cultured in a constant temperature incubator at 28°C, and the antibacterial rates were counted on the 7th to 14th day. The results are shown in the table below, R. albicans LL210 has a significant inhibitory effect on the mycelial growth of Phytophthora tomato ( image 3 ), the bacteriostatic rate when cultivating 7-14d is respectively:

[0040] time...

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Abstract

The present invention relates to albicans LL210, its application and biocontrol agent. The Irpex lacteus LL210 provided by the present invention is classified as Irpex lacteus and has a preservation number of CGMCC No. 21057. The strain is isolated from tomato leaves and is non-toxic and non-pathogenic. The plate confrontation test shows that the strain can effectively inhibit the mycelium growth and spore germination of Botrytis cinerea and Phytophthora infestans, and can be used to antagonize plant pathogenic fungi Botrytis cinerea and / or Phytophthora. The present invention utilizes the biocontrol fungus agent prepared by the albicans LL210 to prevent and control tomato diseases, and the control effects on tomato gray mold and late blight respectively reach 81.35% and 87.68%, and have good disease control effects and are compatible with the environment Well, it provides a new microbial resource for the biological control of plant diseases such as tomato.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Racula albicans LL210, its application, and a biocontrol agent. Background technique [0002] The production mode of protected vegetables in my country has the characteristics of low investment, low energy consumption, intensification, and year-round cultivation, which leads to serious obstacles to continuous cropping and high incidence of diseases, which has become a bottleneck restricting the sustainable utilization of protected vegetables in my country. For example, tomato gray mold caused by Botrytis cinerea infection caused by Fusarium oxysporum infection, tomato late blight caused by Phytophthora, etc. At present, the control of pathogenic microorganisms mainly includes physical control, chemical control and biological control, etc. Most of them still use traditional chemical control, such as the use of organophosphate pesticides, organic arsenic, and benzene p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00A01G22/05C12R1/645
CPCC12N1/14A01N63/30A01G22/05
Inventor 陈双臣刘爱荣毋美娟付亚伦张少杰彭晓华杨政坤耿李佳宏宋智煜赵同淑黄茜茜
Owner HENAN UNIV OF SCI & TECH
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