Method of increasing content of polysaccharide in mycelia of lucid ganoderma by means of fungal polysaccharides, and lucid ganoderma product prepared therefrom

A technology of ganoderma mycelium and fungal polysaccharides, applied in the direction of microorganism-based methods, fungi, biochemical equipment and methods, etc., can solve the problems of low yield of ganoderma polysaccharides, limited effect of ganoderma polysaccharide content, etc., and achieve the goal of breaking polysaccharides The effect of low content, rich nutrition and high polysaccharide content

Active Publication Date: 2018-09-28
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the optimization of liquid fermentation technology mainly focuses on the composition of culture medium, temperature, pH value, etc., and its effect on increasing the polysaccharide content of Ganoderma lucidum is limited. The low yield of polysaccharides in Ganoderma lucidum is still the main bottleneck restricting the industrial fermentation production of Ganoderma lucidum

Method used

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  • Method of increasing content of polysaccharide in mycelia of lucid ganoderma by means of fungal polysaccharides, and lucid ganoderma product prepared therefrom
  • Method of increasing content of polysaccharide in mycelia of lucid ganoderma by means of fungal polysaccharides, and lucid ganoderma product prepared therefrom

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 utilizes fungal polysaccharide to improve the method for polysaccharide content of ganoderma lucidum mycelium

[0035] 1. Preparation of fungal polysaccharides

[0036] 1.1. After activating the Monascus strain, inoculate it in a liquid medium for fermentation, and culture it in a shaker at 160r / min at 26°C for 6 days while avoiding light.

[0037] 1.2. After the cultivation, separate the mycelium, dry it in an oven at 55-65°C to constant weight, and grind it into powder.

[0038] 1.3. Add distilled water to the dry powder of filamentous mycelium at a ratio of 1:20, ultrasonically extract for 10 minutes (50°C, 200W), centrifuge for 20 minutes, and collect the supernatant.

[0039] 1.4. Add distilled water to the residue according to the above ratio, extract at 100°C, combine the supernatants, and add 3 times the volume of 95% ethanol for ethanol precipitation for 24 hours, centrifuge and discard the supernatant to obtain a precipitate.

[0040] 1.5. After...

Embodiment 2

[0049] Embodiment 2 utilizes fungal polysaccharide to improve the method for polysaccharide content of ganoderma mycelia

[0050]The liquid fermentation of Ganoderma lucidum was carried out with reference to the method described in Example 1, the only difference being that: the activated Aspergillus niger strain was inoculated in step 1, and the Aspergillus niger polysaccharide was prepared, and the polysaccharide added to the liquid medium in the step 2 was black Aspergillus polysaccharide.

Embodiment 3

[0051] Embodiment 3 utilizes fungal polysaccharide to improve the method for polysaccharide content of Ganoderma lucidum mycelia

[0052] The liquid fermentation of Ganoderma lucidum was carried out with reference to the method described in Example 1, the only difference being that the activated Fusarium oxysporum strain was inoculated in step 1, and the Fusarium oxysporum polysaccharide was prepared, and the step 2 was added to the liquid medium The polysaccharide is Fusarium oxysporum polysaccharide.

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Abstract

The invention relates to a method of increasing content of polysaccharides in mycelia of lucid ganoderma by means of fungal polysaccharides, and a lucid ganoderma product prepared therefrom. In the method, lucid ganoderma is inoculated on a liquid culture medium for fermenting cultivation, wherein the liquid culture medium contains 30-110 [mu]g / ml of fungal polysaccharides; the fungal polysaccharides are extracted from one or more of monascus, aspergillus niger, fusarium oxysporum and irpex lacteus. The method can increase the content of polysaccharides in culture of the lucid ganoderma by increasing biomass of mycelia and / or content of intercellular polysaccharides in the mycelia during liquid fermentation of the lucid ganoderma. The method breaks a technical bottleneck of low polysaccharide content in a liquid fermentation process of the lucid ganoderma. The lucid ganoderma product is high in polysaccharide content and has great nutritional value.

Description

technical field [0001] The invention relates to the field of fungal culture, in particular to a method for increasing the polysaccharide content of ganoderma mycelia by using fungal polysaccharides and the obtained ganoderma products. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a large fungus belonging to the genus Ganoderma in the family Polyporaceae Basidiomycetes. Ganoderma lucidum, as a large fungus with both medicinal and edible uses, contains a variety of biologically active ingredients. It has a history of medicinal and edible use in my country for more than 2,000 years. [0003] Modern scientific research has found that Ganoderma lucidum polysaccharide is one of the most important active ingredients of Ganoderma lucidum, which can improve the body's immunity, inhibit tumors, promote protein and nucleic acid synthesis, eliminate free radicals in the body, delay aging, resist oxidation, resist hypoxia, resist radiation, Improve liver detoxif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/14C12R1/645
CPCA23L31/00C12N1/14C12P19/04
Inventor 龚文兵彭源德谢纯良朱作华周映君
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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