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Irpex lacteus capable of efficiently degrading lignin

A technology of albacodon and lignin, which is applied in the field of microbial engineering, can solve the problems of high economic cost and poor depolymerization effect, and achieve the effect of short degradation time and simple operation

Active Publication Date: 2020-11-20
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Carrying out the screening of strains that can efficiently degrade lignin, especially those that can efficiently degrade pure lignin, will have important practical significance for the development of industrialized lignin-degrading strains for the biorefinery industry, and will help to solve the problem of lignin pretreatment in the biorefinery process. Problems such as high economic cost and poor depolymerization effect in the processing process

Method used

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  • Irpex lacteus capable of efficiently degrading lignin
  • Irpex lacteus capable of efficiently degrading lignin
  • Irpex lacteus capable of efficiently degrading lignin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Isolation, screening and species identification of Irpex lacteus S-11 strain

[0052] 1. Separation and screening

[0053] The fruiting body of the fungus taken from dead standing trees or fallen trees with good growth potential in Yuelu Mountain, Hunan Province was cleaned with sterile water and wiped with 75% alcohol, and then the fruiting body of the fungus was cut into pieces The 0.5cm×0.5cm bacterial block is connected to the PDA agar medium and cultured in a constant temperature incubator at 28°C.

[0054] After the fungal mycelium grows on the PDA agar medium, cut off the 0.5cm×0.5cm mycelium with a thin layer of medium with an inoculation spatula, and connect it in a new PDA agar medium.

[0055] Obtain pure colonies of microorganisms after repeated inoculation and culture for 2 to 3 generations, inoculate the isolated pure colonies on PDA-aniline blue agar medium and PDA-guaiacol agar medium respectively, and place them in a constant temperature inc...

Embodiment 2

[0061] Example 2: Detection of lignin-degrading enzyme produced by Irpex lacteus S-11

[0062] Inoculate the Irpex lacteus S-11 mycelia obtained in Example 1 onto the PDA-guaiacol agar medium, then place the PDA-guaiacol agar medium upside down at a constant temperature of 28°C In the incubator, cultivate for 3 to 5 days, observe and record the discoloration of the medium around the colony.

[0063] Such as Figure 4~5 As shown, compared with the control without strain inoculation, there is a larger and clear reddish-brown color circle around and at the bottom of the colony on the PDA-guaiacol agar medium inoculated with the bacteria, and the diameter of the color circle is is: 32mm, indicating that the strain has a strong ability to produce lignin-degrading enzymes.

Embodiment 3

[0064] Example 3: Detection of peroxidase produced by Irpex lacteus S-11 strain

[0065] The Irpex lacteus (Irpex lacteus) S-11 mycelium obtained in Example 1 was inoculated on the PDA-aniline blue agar medium, and then the PDA-aniline blue agar medium was placed upside down in a constant temperature incubator at 28°C, After culturing for 3 to 5 days, observe and record the decolorization of the culture medium around the colony.

[0066] Such as Figure 6-Figure 7 As shown, compared with the control, there is a large and clear fading circle around and at the bottom of the PDA-aniline blue agar medium inoculated with the bacterium. The diameter of the fading circle is: 53mm, and the mycelium still presents natural color, This shows that the strain can utilize and metabolize aniline blue, and that the strain has a strong ability to produce lignin peroxidase or manganese peroxidase.

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Abstract

The invention discloses an irpex lacteus S-11 capable of efficiently degrading lignin, and belongs to the technical field of microbial engineering. A liquid medium containing 4 g / L lignin is inoculated with the strain, the strain is cultured at 28 DEG C at 180 rpm for 11 days, and the degradation rate and decolorization rate of the strain for lignin reach 33.81% and 41.79% respectively. The methodfor degrading the lignin by using the strain is convenient to operate, low in cost and suitable for biological pretreatment of lignin in biorefinery industry. The strain has the degradation rate of apure lignin substrate higher than that of same-species strains reported at home and abroad, is shorter in degradation time and is suitable for biological pretreatment application in the biorefinery industry.

Description

technical field [0001] The invention relates to a bacterium albicans that efficiently degrades lignin, and belongs to the technical field of microbial engineering. Background technique [0002] Lignocellulosic biomass is the most abundant renewable resource on earth. It comes from a wide range of sources and produces low carbon emissions. It can be used to obtain clean energy and high value-added chemicals. Research on the conversion of lignocellulosic biomass into biofuels and high-value chemicals through biofermentation has attracted more and more attention. However, the biotransformation of lignocellulosic biomass is affected by its complex structure, which seriously restricts its industrial refining process. Lignocellulose is mainly composed of cellulose, hemicellulose, and lignin. In lignocellulose structure, lignin chemically interacts with cellulose and hemicellulose through covalent and non-covalent bonds to form a complex heterogeneous structure. The three-dimensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C08H7/00C12N9/08C12R1/645
CPCC08H6/00C12N9/0065C12Y111/01013C12R2001/645C12N1/145
Inventor 马江山刘高强张家顺刘勇男王晓玲
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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