Preparation method and application of microbial straw degradation microbial agent

A technology of degrading bacteria and microorganisms, which is applied in the field of preparation of microbial straw degrading bacterial agents, can solve the problems of short returning time, shortening the time of straw returning to the field, and the limitation of the process of straw returning to the field naturally, so as to improve the soil micro-environment As well as the ecological community structure, solving the technical problems of straw returning to the field and soil improvement, and the effect of high-efficiency degradation

Inactive Publication Date: 2020-03-27
南京朴厚生态科技有限公司
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Problems solved by technology

Based on the principle of "using straw to benefit, discarding to harm", under the conditions of modern agricultural production, the efficient development and intensive utilization of straw resources are of great importance to the soil fertility, soil and water conservation and environmental safety in the entire agricultural ecosystem. The effect of straw returning on the supply and storage of soil nutrients, the content of soil organic matter, and the improvement of soil ecological environment and quality has been confirmed a lot. There are still many difficulties in the field, especially the dry and cold climate in autumn and winter in northern China and the soil texture in the south, which greatly restricts the process of returning straw to the field naturally. The internal cause of the difficulty in returning straw to the field lies in the structural characteristics of the straw itself. External factors such as the short time for returning to the field, the lack of popularization of supporting machinery for returning to the field, and the limitation of returning technology also largely affect the smooth implementation of straw returning to the field. Therefore, judging from the current status of straw returning in vast areas of China , How to treat a large amount of straw ecologically, speed up the straw decomposition time without affecting the normal growth of the next crop is the crux of the difficulty of straw returning to the field. The degrading bacteria agent is the core of straw rot promotion and returning to the field. The application of straw degrading bacteria agent can not only effectively decompose the structural components of straw and greatly shorten the time of straw returning to the field, but also the beneficial microorganisms in the agent can improve soil microbiology. environment and ecological community structure, thus contributing to the improvement and improvement of the soil ecological environment and soil quality. The current straw degrading bacteria agent does have a significant role in promoting the decomposition of straw, but the problem is that it involves multiple microorganisms The fermented seed production process is relatively cumbersome and the production cost is high. In the specific use process, different strains may compete or antagonize each other, which will have adverse effects on the growth and development of the strains. Moreover, the currently used strains produce enzymes The level is not high, the enzyme system is single (mainly endo-P-glucanase), it is difficult to efficiently degrade the lignocellulosic structure of the straw, so that the straw degradation process is too long, so people need a microbial straw degradation Bacteria to solve the above problems

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  • Preparation method and application of microbial straw degradation microbial agent

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Embodiment 1

[0034] The preparation method of microbial straw degrading bacteria agent comprises the following steps:

[0035]1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;

[0036] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:

[0037] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;

[0038] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cult...

Embodiment 2

[0042] The preparation method of microbial straw degrading bacteria agent comprises the following steps:

[0043] 1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;

[0044] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:

[0045] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;

[0046] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cul...

Embodiment 3

[0050] The preparation method of microbial straw degrading bacteria agent comprises the following steps:

[0051] 1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;

[0052] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:

[0053] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;

[0054] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cul...

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Abstract

The invention discloses a preparation method and an application of a microbial straw degradation microbial agent. The method comprises the steps: firstly, preparing strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum and Schizophyllum comune; taking the prepared strains, respectively activating, culturing and fermenting; and finally, obtaining a fermentation liquids of each strain,and treating and compounding the fermentation liquids to obtain a soil conditioner. The method is scientific and reasonable, safe and convenient in use; different tool microorganisms are respectivelyand independently cultured and then mixed and compounded according to a concentration ratio of 1:1:1:1, and an optimal microbial straw degradation microbial agent is obtained; the microbial agent isinoculated to treated plant straw for continuous fermentation; and the fermentation product is subjected to solid-liquid separation and innocent treatment and is dried at the temperature of 60 DEG C to form the soil conditioner. Through optimized tool microorganism proportioning, the technical problems of straw returning to the field and soil improvement are solved while high-efficiency degradation of the plant straw is realized.

Description

technical field [0001] The invention relates to the technical field of straw degradation, in particular to a preparation method and application of a microbial straw degradation bacterial agent. Background technique [0002] my country is both a big agricultural country and a big straw producer. Based on the principle of "using straw to benefit, discarding to harm", under the conditions of modern agricultural production, the efficient development and intensive utilization of straw resources are of great importance to the soil fertility, soil and water conservation and environmental safety in the entire agricultural ecosystem. The effect of straw returning on the supply and storage of soil nutrients, the content of soil organic matter, and the improvement of soil ecological environment and quality has been confirmed a lot. There are still many difficulties in the field, especially the dry and cold climate in autumn and winter in northern China and the soil texture in the sout...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14C12N1/14C12R1/66C12R1/80C12R1/645C09K101/00
CPCC09K17/14C09K2101/00C12N1/14
Inventor 王欢欢钟晶晶
Owner 南京朴厚生态科技有限公司
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