Complex microbial inoculant for degrading corn straws at low temperature

A technology of compound bacterial agent and corn stalks, which is applied in the direction of bacteria, fungi, and microorganism-based methods, can solve the problem that the strains are not suitable for application under low temperature conditions in northern regions, and achieve accelerated maturity time, excellent stability, and high degradation rate effect

Pending Publication Date: 2022-04-01
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many domestic straw decomposing agents, but the straw-degrading bacteria contained in them mainly function under medium-high temperature or normal temperature conditions. Due to the limitation of ambient temperature or bacterial growth rate, these strains are not suitable for low-temperature conditions in northern regions. application

Method used

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  • Complex microbial inoculant for degrading corn straws at low temperature
  • Complex microbial inoculant for degrading corn straws at low temperature
  • Complex microbial inoculant for degrading corn straws at low temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Screening and mutagenesis of embodiment 1 Bacillus cereus

[0033] (1) Separation and screening

[0034] Collect 5g soil samples from the forest area of ​​Changbai Mountain Nature Reserve, dissolve them in 100mL prepared saline solution, shake and culture at 10°C and 180rpm for 2-4h, the saline solution is 0.006mM FeSO 4 ·7H 2 O; 0.01 mM CaCO 3 ·7H 2 O; 0.08 mM MgSO 4 ·7H 2 O; 0.07mM MnSO 4 ·7H 2 O and 0.006mM ZnSO 4 ·7H 2 O composition.

[0035] 1mL of the mixed soil sample salt solution was subjected to different gradients (10 -2 、10 -3 、10 -4 ) dilution, take 200ul of the diluted solution and spread it on the R2a solid plate respectively, place it in a constant temperature incubator at 10°C for static culture for 7-10 days, and place it on the surface of the solid medium of the TSB plate.

[0036]TSB plate solid medium contains 0.5g yeast extract powder, 0.5g peptone, 0.5g casein hydrolyzate, 0.5g glucose, 0.5g soluble starch, 0.3g potassium dihydrogen p...

Embodiment 2

[0045] Antagonism test of each strain

[0046] In addition to the above-mentioned Bacillus cereus TK-2,

[0047] The preservation number of Bacillus subtilis used in the present invention is compiled as: CCTCC M: 2019185; the preservation number of Bacillus licheniformis is compiled as: CGMCC 1.8805; the preservation number of Bacillus megaterium is compiled as: CGMCC 1.7146; Pseudomonas berry The preservation number of Trichoderma viride is: CGMCC 3.3744; the preservation number of Aspergillus niger is: CGMCC 3.17612; the preservation number of white rot fungus is ACCC 30942; The serial number is: CGMCC5.939.

[0048] The antagonism between the various strains to be constructed by using the plate cross-hatching method and the spot method is determined, and the specific methods are as follows:

[0049] (1) Antagonism experiment between bacteria

[0050] Streak the bacteria crosswise in pairs on the LB plate, and incubate them upside down at a constant temperature of 15°C fo...

Embodiment 3

[0057] Determination of the degradation rate of each strain on straw liquid fermentation:

[0058] Weigh 5.00g of corn stalks, crush them through a 20-mesh sieve, and prepare Hutchinson medium (g / L), and inoculate 9 strains in the liquid fermentation medium according to the inoculation amount of 1% for shaking culture After 48 hours, transfer to inorganic salt culture medium, shake and culture at 15°C and 160r / min for 15 days, set 3 repeated experiments for each treatment, take the fermented product after drying and weigh it, and measure the degradation of straw by each strain Rate. Such as image 3 As shown, the degradation effect of Aspergillus niger was the best, and the degradation rate reached 40.75%, which was 15.54% higher than the slowest Pseudomonas berry. Followed by R. albicans and white rot fungus, the degradation rates were 38.77% and 38.84%.

[0059] Based on six strains of Bacillus cereus TK-2, Pseudomonas berry, Bacillus subtilis, Bacillus licheniformis, Bac...

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Abstract

The invention relates to a complex microbial inoculant for degrading corn straws at low temperature. The complex microbial inoculant is prepared from bacillus cereus TK-2 (Bacillus cereus), bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus megatherium (Bacillus megatherium), Pseudomonas fragi (Pseudomonas fragi), Trichoderma viride (Trichoderma viride), Aspergillus niger (Aspergillus niger), Phanerochaete and Irpex lacteus (Irpex lacteus), and the complex microbial inoculant can be used for degrading corn straws at low temperature. The complex microbial inoculant formed by nine strains including bacillus cereus has the degradation efficiency of 38.71% at the indoor constant temperature of 15 DEG C, can efficiently degrade the corn straw and accelerate the decomposition time in the natural environment of 1-10 DEG C, has a high degradation rate, has the degradation rate of 35.93% after 30 days, and has excellent stability.

Description

technical field [0001] The invention relates to the technical field of microorganisms and applications thereof, in particular to a composite bacterial agent for degrading corn stalks at low temperature. Background technique [0002] Northeast China is the largest corn production area in my country, and tens of millions of tons of corn stalks can be harvested every year. Corn stalks generally refer to the remaining part after harvesting corn kernels. In the past, they were considered of little use, and most of them were directly discarded or even incinerated. Not only was there no reasonable development and utilization, but it also caused serious pollution to the environment. However, corn stalks are a biomass resource with great potential. It is rich in nitrogen, phosphorus, potassium, calcium, magnesium, and organic matter. Using corn stalks to return to the field can return the nutrients to the soil and alleviate the lack of soil nutrients. Increase soil fertility; return...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/00C05F11/08C05F17/20C12R1/085C12R1/125C12R1/10C12R1/11C12R1/38C12R1/885C12R1/685C12R1/645
CPCY02W30/40
Inventor 张斯童张宏泽苏煦涵陈欢王刚孙旸陈光
Owner JILIN AGRICULTURAL UNIV
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