Separation method of botrytis cinerea

A technology for tomato botrytis cinerea and tomato botrytis cinerea, which is applied in the field of phytopathology, can solve problems such as time-consuming, affecting the efficiency and effect of separation work, and achieves the effects of short time consumption, simple and easy separation operation, and low labor consumption.

Active Publication Date: 2021-06-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of method needs to carry out the spore quantification and dilution operation process of the spore liquid, which is more troublesome, and it is easy to be contaminated by bacteria, which will affect the efficiency and effect of the separation work.

Method used

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  • Separation method of botrytis cinerea
  • Separation method of botrytis cinerea
  • Separation method of botrytis cinerea

Examples

Experimental program
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Effect test

preparation example Construction

[0039] 3. Preparation of Spore Dragging Plates

[0040] Prepare as follows.

[0041] First prepare the agar plate matrix:

[0042] According to the ratio of 15.0 g of agar and 1000.0 ml of water, the agar matrix containing conventional nutrition was prepared. The conventional nutrition of Botrytis cinerea can use potato dextrose (PD) medium, add in the agar matrix according to the conventional dosage, become the agar plate matrix containing nutrition after conventional high temperature and high pressure sterilization. In actual application, the amount of agar used can be determined according to the quality of agar purchased from the market. The amount of agar used per liter of substrate can vary from 10.0g to 15.0g. In principle, the amount used is small and cut into small agar strips after condensation , it is advisable that the small agar strips still have good elasticity.

[0043] Then invert the surface-cleaned agar plate:

[0044] The agar content of the conventional ...

Embodiment 1

[0060] Adopt a kind of separation method of Botrytis cinerea of ​​tomato of the present invention, isolate the Botrytis cinerea of ​​tomato of Standard Sample 1 of Botrytis cinerea, the result of isolation and culture is as follows: Image 6 shown.

Embodiment 2

[0062] Adopt a kind of separation method of Botrytis cinerea of ​​tomato of the present invention, isolate the Botrytis cinerea of ​​tomato of Standard Sample 2 of Botrytis cinerea, the result of separation and culture is as follows: Figure 7 shown.

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Abstract

The invention discloses a separation method of botrytis cinerea. According to the method, single conidia are directly separated from a botrytis cinerea standard sample by adopting a fine, soft and elastic spore dragging filament and are cultured to form a botrytis cinerea strain with single genetic composition. The implementation and operation steps of the isolated culture are as follows: (1) collecting a botrytis cinerea standard sample; (2) firing spore dragging and dispersing filaments; (3) preparing a spore dragging and dispersing plate; (4) dragging and dispersing conidia; (5) cutting small square blocks of single spores; and (6) forming a monospore isolated strain. The method has the following advantages: (1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; (2) single spore strains can be separated and obtained at one time; (3) the separation work consumes less time and labor; and (4) the working efficiency of botrytis cinerea separation and the reliability of the separation result can be obviously improved.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for isolating Botrytis cinerea of ​​tomato. Background technique [0002] Tomato cinerea is a major disease of tomato, a staple vegetable in my country, which is caused by the infection of Botrytis cinerea. Carrying out biological research on Botrytis cinerea is an important basis for the control of Botrytis cinerea. The prerequisite for carrying out biological research on Botrytis cinerea is to have materials of Botrytis cinerea. General laboratories often need to isolate Botrytis cinerea from disease standard samples. [0003] The genetic composition of Botrytis cinerea is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain materials of Botrytis cinerea with a single genetic composition, or monoclonal strain materials. It is generally believed in the art that...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12N1/02C12M1/26C03B23/047C12R1/645
CPCC12N3/00C12N1/14C12N1/02C12M33/02C03B23/047
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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