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Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof

A technology of boxwood and bacillus, applied in the application, bacteria, biocide and other directions, can solve the problems of pollution, bacteria resistance environment, etc.

Active Publication Date: 2016-05-04
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the control of Euonymus anthracnose mainly relies on chemical control, which may easily lead to problems such as drug resistance of bacteria and environmental pollution.

Method used

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  • Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof
  • Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof
  • Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Effect of HYEB5-6 on mycelial growth of Euonymus anthracnose

[0026] Inoculate the bacteria block of Euonymus anthracnose in the center of the PDA plate. At the same time, the purified HYEB5-6 was streaked at the left and right positions of the bacteria cake, and the control plate was only inoculated with the pathogenic fungus cake. The test was repeated 3 times. The inoculated plates were cultured in an incubator at 28°C for 3 days, and the antibacterial effect of HYEB5-6 on Euonymus anthracnose was determined. The result is as figure 2 , HYEB5-6 showed obvious inhibitory effect on the mycelial growth of Euonymus anthracnose.

Embodiment 2

[0027] Example 2: Inhibitory effect of HYEB5-6 on the mycelium growth of several plant pathogens

[0028] The pathogenic bacteria block was inoculated in the center of the PDA plate, and at the same time, the purified HYEB5-6 was spotted at the four corners 30 mm away from the bacterial cake. The control plate was only inoculated with the pathogenic fungal cake, and the test was repeated 3 times. Place the inoculated plates in an incubator at 28°C for 3-5 days, and measure the antibacterial effect of HYEB5-6 on the other seven pathogenic bacteria. The result is as image 3 , it can be seen from the figure that HYEB5-6 has obvious inhibitory effect on 7 kinds of pathogenic bacteria.

Embodiment 3

[0029] Example 3: Effects of metabolites of HYEB5-6 on the germination of conidia and the growth of germ tube mycelium of Euonymus anthracnose

[0030] A single colony of activated HYEB5-6 was inoculated into 100 mL of liquid NA medium, and cultured with shaking at 250 rpm at 28°C for 12 hours. The supernatant was collected after centrifugation, and the supernatant was filtered and sterilized through a 0.22 μm filter to obtain a sterile filtrate containing HYEB5-6 metabolites, which was diluted 5 times with sterile water for use. Inoculate Euonymus anthracnose bacteria HYCG2-3 on a fresh PDA plate, culture at 28°C for 5 days, cut off mycelium pieces with a diameter of 5mm from the edge of the colony and inoculate them into 100mL CMC liquid medium, culture with shaking at 28°C and 250rmp 3 days. Collect the conidia of Euonymus anthracnose and adjust the concentration to 10 6 individual / mL. Mix the above sterile filtrate with an equal volume to a concentration of 10 6After m...

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Abstract

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.

Description

technical field [0001] The invention belongs to the field of microbial pesticides, in particular to an endophytic bacterium methylotrophic bacillus of Euonymus euphratica and its application. Background technique [0002] Plant anthracnose is a kind of worldwide disease with wide geographical distribution. Anthracnose damage has been reported from monocots to dicotyledons, from gymnosperms to angiosperms. The disease infects plant leaves, stems, fruits, etc., causing huge economic losses. The asexual stage of anthracnose belongs to Eumycota, Deuteromycotina, Coelomycetes, Melanconiales, Colletotrichum Corda; the sexual form belongs to Fungi (Eumycota), Ascomycota (Ascomycota), Sclerotinia (Pyrenomyetes), Sphaeriales (Sphaeriales), Glomerella. The plant anthracnose fungus overwinters with mycelium and conidia discs with the diseased residue or in the soil, becoming the primary source of infection of the disease. When the temperature and humidity conditions are suitable, th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 黄麟侯瑶李秋成叶建仁李国倩何燕娇姜可婧刘璐龚馨玥
Owner NANJING FORESTRY UNIV
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