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Bacillus new species with Colletotrichum fragariae inhibition activity, and application of bacillus new species

A technology of bacillus and anthracnose, applied in the field of microorganisms, can solve the problem of less research on broad-spectrum antifungal bacillus, achieve good development and application prospects, stabilize broad-spectrum antagonistic activity, and reduce the effect of weight loss rate

Active Publication Date: 2021-07-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

At present, there are many researches on the biocontrol of postharvest strawberry fruit at home and abroad, but there are few studies on the broad-spectrum antifungal Bacillus and its biocontrol mechanism in strawberry fruit storage

Method used

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  • Bacillus new species with Colletotrichum fragariae inhibition activity, and application of bacillus new species
  • Bacillus new species with Colletotrichum fragariae inhibition activity, and application of bacillus new species
  • Bacillus new species with Colletotrichum fragariae inhibition activity, and application of bacillus new species

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific examples, so as to better understand the present invention.

[0023] The invention provides a bacillus, which is a new species of the genus Bacillus, named: Bacillussp.Nov.QN1NO-4, which was preserved in the China Center for Type Culture Collection on March 30, 2021, and the preservation number is CCTCCM2021306 . The bacillus Bacillus sp. Nov. QN1NO-4 (hereinafter referred to as "QN1NO-4") of the present invention is isolated and screened from noni fruit.

[0024] All the data in the embodiment of the present invention were subjected to one-way analysis of variance (ANOVA) using SPSS version 22 (SPSS Inc., Chicago, IL, USA). LSD multiple comparison data was used to analyze the significant difference (P<0.05). The data obtained from three repeated experiments are represented by standard deviation ±.

[0025] 1 Experimental materials

[0026] Strawberry (Fragaria×ananassa Duch.var.Zhang...

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Abstract

The invention provides a bacillus which is a new species of the bacillus, is called after Bacillus sp. Nov. QN1NO-4 and is collected in the China Center for Type Culture Collection on March 30, 2021 with the collection number of CCTCC M 2021306. The bacillus has stable broad-spectrum antagonistic activity and has a powerful anti-bacteria function for common crop pathogenic fungi, including Fusarium graminearum, Curvularia lunata, Colletotrichum gloeosporioides, Fusarium oxysporum f. sp. cucumebrium, Colletotrichum fragariae, Colletotrichum gloeosporioides penz., Pyricularia oryzae, Colletotrichum capsici, Alternaria sp., Colletotrichum acutatum and the like, the fermentation extract of the bacillus can effectively prevent pathogenic hyphae from infecting isolated fruits and the like, the weight loss ratio of the fruit is lowered, soluble solid matters can be favorably kept, the safe source of the effective ingredient of a biocontrol preparation is increased, a new field is widened for preventing and treating various plant diseases, including the Colletotrichum fragariae and the like, and the bacillus has a good development and application prospect.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a new bacillus strain with strawberry anthracnose inhibitory activity and application thereof. Background technique [0002] Strawberry (Fragaria × ananassa Duch.) is one of the most popular fruits, with an annual production of more than 6.1 million tons since 2010 and a total production value of over US$12.3 billion (Zhimo et al., 2021). However, due to the soft texture of strawberry fruit, it is susceptible to mechanical damage and bacterial infection and rot, which limits the postharvest storage period and shelf life (Rico et al., 2019). Strawberry anthracnose, caused by the fungus Colletotrichum fragariae, is one of the most serious diseases of cultivated strawberries (Miller-Butler et al., 2018), and the use of chemical fungicides such as thiabendazole or imazalil is a thing of the past The main postharvest disease control method for strawberry in the past 30 years (Duka...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B7/155A23B7/154A23B9/28A23B9/26C12R1/07
CPCA23B7/155A23B7/154A23B9/28A23B9/26
Inventor 张妙宜王尉谢江辉周登博起登凤李凯赵炎坤陈宇丰
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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