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A marine fungus fermented extract and its application as an anti-senile dementia drug

A technology of marine fungi and extracts, which is applied in the application of anti-senile dementia drugs and the field of marine fungal fermentation extracts, to achieve the effects of significantly inhibiting activity, improving biological activity, and strong antioxidant activity

Active Publication Date: 2019-02-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor previously found that the fermented extract of the kelp endophytic fungus ML-X from Qingdao sea area showed certain inhibitory activity on acetylcholinesterase, and optimized its fermentation conditions, but its inhibitory activity on acetylcholinesterase was only 28.91% ±0.25%

Method used

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  • A marine fungus fermented extract and its application as an anti-senile dementia drug
  • A marine fungus fermented extract and its application as an anti-senile dementia drug
  • A marine fungus fermented extract and its application as an anti-senile dementia drug

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Experimental program
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Effect test

Embodiment 1

[0034] (1) Under sterile conditions, the marine fungus Alternaria sp.W-1 was inoculated into PD liquid medium, and cultured on a constant temperature shaker at a temperature of 28°C, the speed of the shaker was 160r / min, and the culture time was 3 days. get seed liquid;

[0035] (2) Inoculate the seed solution obtained in step (1) into the Cha's culture medium (the fermentation volume is 2.0 L), the inoculum volume is 10%, the cultivation temperature is 28° C., and the cultivation time is 10 days to obtain a fermented product;

[0036] (3) Filter the fermented product obtained in step (2) with 4 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 3 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the obtained Said extract (2.8g, referred to as product A).

Embodiment 2

[0037] The acetylcholinesterase inhibitory activity (AchE) of embodiment 2 product A and the mensuration of antioxidant

[0038] Take an appropriate amount of product A and dissolve it in DMSO to prepare a solution with a mass concentration of 5 mg / mL. The method for measuring the AChE activity inhibition rate refers to the improved Ellman method, and the method for measuring the DPPH free radical scavenging ability and superoxide anion scavenging ability refers to the literature. The results are shown in Table 1

[0039] Table 1

[0040]

Embodiment 3

[0041] Example 3 Marine fungus Alternaria sp.W-1 fermentation condition optimization

[0042] Follow the same steps to prepare the seed solution: under sterile conditions, inoculate the marine fungus Alternaria sp.W-1 into PD liquid medium, and culture it on a constant temperature shaker with shaking at a temperature of 25-30°C and a shaker speed of 150- 180r / min, the cultivation time is 2-4d, to obtain the seed liquid; follow the same steps to obtain the extract from the fermented product: the fermented product is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator and then washed with acetic acid Ethyl ester was extracted for 2-4 times, the organic phases were combined, and concentrated by a rotary evaporator to obtain the extract. Investigate the effects of temperature, pH, inoculum size, and fermentation time on the acetylcholinesterase inhibitory activity of the fermentation extract in the fermentation culture (th...

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Abstract

The present invention relates to a marine fungus fermented extract and its application as an anti-senile dementia drug. The extract is prepared by the following steps: (1) under sterile conditions, the marine fungus Alternaria sp.W‑1 is inoculated into PD In the liquid medium, vibrate culture on a constant temperature shaker, the temperature is 25-30°C, the speed of the shaker is 150-180r / min, and the culture time is 2-4d to obtain a seed solution; (2) the seeds obtained in step (1) The solution is inoculated into the Cha's medium, the pH of the medium is 4.0-8.0, the inoculum size is 5-20%, the culture temperature is 22-34°C, and the culture time is 6-14d to obtain a fermented product; (3) the step ( 2) The fermented product obtained is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 2-4 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the extracted thing.

Description

technical field [0001] The invention belongs to the field of marine microorganisms and marine medicines, and in particular relates to a fermented extract of marine fungi and its application as an anti-senile dementia medicine. Background technique [0002] Alzheimer's disease (AD), namely senile dementia, is a chronic degenerative disease of the brain nervous system. Its main clinical manifestations are gradual decline of memory, cognitive dysfunction, apathy, computational impairment, abnormal behavior, and social impairment. Alzheimer's disease leads to mental disability of the elderly, which has become a serious social problem. Current studies have shown that there are many causes of Alzheimer's disease, mainly including cholinergic deficiency theory, oxidative stress theory, gene mutation theory, and inflammatory factor theory. The "cholinergic deficiency theory" advocates that cholinergic neurotransmitters in the brain center are related to memory and cognitive functi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A61K36/06A61P25/28C12R1/645
CPCA61K36/06C12P1/02C12N1/145C12R2001/645
Inventor 王凤舞申丽
Owner QINGDAO AGRI UNIV
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