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Fungus of alternaria sp., preparation method of secondary metabolite of fungus and application of secondary metabolite as microbial acaricide

A technology of secondary metabolites, Alternaria sp., applied in the preparation of pesticides, Alternaria sp. fungi, the field of isolation of Alternaria fungi, can solve the problem of phytotoxicity and resistant pests No matter how rampant, low mite killing effect and other problems

Inactive Publication Date: 2015-01-14
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few acaricidal microbial pesticides currently on the market (Ni Jueping, Research and Development of Insecticides and Acaricides since 2000, 2009, 13(3), 1-7.)

Method used

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  • Fungus of alternaria sp., preparation method of secondary metabolite of fungus and application of secondary metabolite as microbial acaricide
  • Fungus of alternaria sp., preparation method of secondary metabolite of fungus and application of secondary metabolite as microbial acaricide

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Experimental program
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Embodiment Construction

[0055] The isolation, identification and acaricidal activity of the Alternaria fungus of the present invention will be further described through specific examples below. This method is a conventional method unless otherwise specified.

[0056] 1. Isolation of Alternaria fungi (strains)

[0057] Weigh 1 g of alfalfa seeds and immerse in 1 volume of 1 mL of alcohol, shake fully for 10 s, and filter off the alcohol; add 1 volume of 1 mL of 10% sodium hypochlorite solution, shake fully for 5 min, filter out the sodium hypochlorite solution, and rinse repeatedly with sterile water for 5- After 6 times, the solution was filtered off, and 800 μL of water was added to grind the seeds to powder to obtain a tissue suspension; then the tissue suspension was serially diluted in units of 10 times the volume to obtain a volume concentration of 1×10 -4 , 1×10 -5 , 1×10 -6 , 1×10 -7 , 1×10 -8 serial dilutions. Take 0.1 mL of diluents of different concentrations and spread evenly on diff...

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Abstract

The invention discloses an endophytic fungus of alternaria sp. separated from a medicago seed, wherein the endophytic fungus is collected with the number of CGMCCNo.9277. A secondary metabolite of the fungus has remarkable contact activity for agricultural harmful mites, but has no any influences to crops at a relatively low concentration. Therefore, the secondary metabolite serving as a safe, nontoxic and environment-friendly microbial acaricide has significance and favorable application prospect in preparation of a miticide.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and relates to a strain of Alternaria Alternaria sp. Fungus; the present invention also relates to the isolation method of the Alternaria fungus; the present invention also relates to the application of the secondary metabolite of the Alternaria fungus as a microbial source acaricide in the preparation of pesticides. Background technique [0002] Mites belong to the class Arachnida and the subclass Acari. There are about 300,000 to 500,000 species in the world, and they are the second largest biological group in the animal kingdom after Insecta. The herbivorous agricultural mites are found on crops all over the world, and the damage is becoming more and more serious. Agricultural pest mites are species that constantly invade temporary habitats and are highly adaptable to short-lived living environments. They are typical R-type pests due to their small size, strong reproductive capaci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P7/02C12R1/645
CPCA01N63/30C12N1/02C12P1/02C12N1/145C12R2001/645
Inventor 刘权傅华南志标
Owner LANZHOU UNIVERSITY
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