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A kelp endophytic fungus and its application in the preparation of biologically active extracts

A technology of biological activity and extract, applied in the field of marine microorganisms, to achieve the effect of significantly inhibiting activity, improving biological activity, and strong antioxidant activity

Inactive Publication Date: 2019-04-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor previously found that the fermented extract of the kelp endophytic fungus ML-X from Qingdao sea area showed certain inhibitory activity on acetylcholinesterase, and optimized its fermentation conditions, but its inhibitory activity on acetylcholinesterase was only 28.91% ±0.25%

Method used

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  • A kelp endophytic fungus and its application in the preparation of biologically active extracts
  • A kelp endophytic fungus and its application in the preparation of biologically active extracts
  • A kelp endophytic fungus and its application in the preparation of biologically active extracts

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Under sterile conditions, the marine fungus Alternaria sp.W-1 was inoculated into PD liquid medium, and cultured on a constant temperature shaker at a temperature of 28°C, the speed of the shaker was 160r / min, and the culture time was 3 days. get seed liquid;

[0035] (2) Inoculate the seed solution obtained in step (1) into the Cha's culture medium (the fermentation volume is 2.0 L), the inoculum volume is 10%, the cultivation temperature is 28° C., and the cultivation time is 10 days to obtain a fermented product;

[0036] (3) Filter the fermented product obtained in step (2) with 4 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 3 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the obtained Said extract (2.8g, referred to as product A).

Embodiment 2

[0037] The acetylcholinesterase inhibitory activity (AchE) of embodiment 2 product A and the mensuration of antioxidant

[0038] Take an appropriate amount of product A and dissolve it in DMSO to prepare a solution with a mass concentration of 5 mg / mL. The method for measuring the AChE activity inhibition rate refers to the improved Ellman method, and the method for measuring the DPPH free radical scavenging ability and superoxide anion scavenging ability refers to the literature. The results are shown in Table 1

[0039] Table 1

[0040]

Embodiment 3

[0041] Example 3 Marine fungus Alternaria sp.W-1 fermentation condition optimization

[0042] Follow the same steps to prepare the seed solution: under sterile conditions, inoculate the marine fungus Alternaria sp.W-1 into PD liquid medium, and culture it on a constant temperature shaker with shaking at 25-30°C and a shaker speed of 150- 180r / min, the cultivation time is 2-4d, to obtain the seed liquid; follow the same steps to obtain the extract from the fermented product: the fermented product is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator and then washed with acetic acid Ethyl ester was extracted for 2-4 times, the organic phases were combined, and concentrated by a rotary evaporator to obtain the extract. Investigate the effects of temperature, pH, inoculum size, and fermentation time on the acetylcholinesterase inhibitory activity of the fermentation extract in the fermentation culture (that is, in step (2...

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Abstract

The present invention relates to a kelp endophytic fungus and its application in the preparation of biologically active extracts. The strain preservation information of the kelp endophytic fungus is: name of depository unit: General Microbiology Center of China Microbial Strain Preservation Management Committee; preservation Unit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; date of deposit: January 2, 2018; deposit number: CGMCC No.15181; classification name: Alternaria sp.

Description

technical field [0001] The invention belongs to the field of marine microorganisms, and in particular relates to a kelp endophytic fungus and its application in the preparation of biologically active extracts. Background technique [0002] Alzheimer's disease (AD), namely senile dementia, is a chronic degenerative disease of the brain nervous system. Its main clinical manifestations are gradual decline of memory, cognitive dysfunction, apathy, computational impairment, abnormal behavior, and social impairment. Alzheimer's disease leads to mental disability of the elderly, which has become a serious social problem. Current studies have shown that there are many causes of Alzheimer's disease, mainly including cholinergic deficiency theory, oxidative stress theory, gene mutation theory, and inflammatory factor theory. The "cholinergic deficiency theory" advocates that cholinergic neurotransmitters in the brain center are related to memory and cognitive functions. The choliner...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/06A61P25/28A61P39/06C12R1/645
CPCA61K36/06C12N1/145C12R2001/645
Inventor 王凤舞申丽田淑娟郭浩梁瑞
Owner QINGDAO AGRI UNIV
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