Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof

Abstract

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

Description

technical field [0001] The present invention relates to Alternaria strain and its culture method and application, particularly relate to a kind of Alternaria strain that can produce α-L-rhamnosidase (EC 3.2.1.40) and its culture method and its use in the hydrolysis of naringin application. technical background [0002] Naringin is the main bitter substance in the processing of citrus fruit juices. The removal of naringin is mainly divided into physical method and enzymatic method. The physical method has disadvantages such as poor controllability, affecting product quality and flavor, and complicated process, while the enzymatic method has attracted more and more attention due to its advantages of simple operation, easy control, and better retention of product nutrition and flavor. α-L-rhamnosidase can hydrolyze naringin, and the bitterness of the hydrolyzed product is much smaller than that of naringin, thus improving the taste of fruit juice. [0003] Most of the reporte...

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Problems solved by technology

[0004] In view of the limited species of fungal microorganisms producing α-L-rhamnosidase in the industrial application of existing microbial enzymatic methods, especially the limited species of microorganisms that produce α-L-rhamnosidase with strong hydrolytic activity and good stability , the object of the present invention is to provide a new isolated Alternaria strain that can produce strong hydrolytic activity and good stability of α-L-rhamnosidase, the α-L-rhamnosidase produced by this bacteria can be Efficiently catalyzes the hydrolysis of naringin

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