Application of momordica grosvenori proteolytic enzyme in preparing polypeptide and amino acid with protolysate
A protease hydrolysis and protein hydrolysis technology is applied in the application field of Luo Han Guo protease in the preparation of polypeptides and amino acids by hydrolyzing protein, which can solve the problems of low degree of hydrolysis, limited enzyme activity, heavy bitterness of hydrolyzed products, etc. Few, safe effects
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Embodiment 1
[0020] (1) Use soybean protein isolate or soybean meal containing soybean protein to prepare a soybean protein solution with a concentration of 3%, and after treating at 85°C for 15 minutes, adjust the pH value to 8.0;
[0021] (2) Cool to 50°C and add Luo Han Guo protease or fresh Luo Han fruit juice containing Luo Han Guo protease to make the enzyme concentration 1500U / g (protein), keep at 50°C for enzymolysis reaction, and continuously add 0.1mol / sodium hydroxide solution at the same time, Keep the pH value of the soybean protein solution constant and react for 60 minutes;
[0022] (3) After the reaction, rapidly heat to 90°C to terminate the enzyme reaction;
[0023] (4) filtering to obtain soybean polypeptide clarified liquid;
[0024] (5) The above-mentioned filtrate is processed by known methods such as ultrafiltration, column chromatography, and freeze-drying to obtain soybean polypeptide and amino acid products.
Embodiment 2
[0026] (1) Prepare a 3% corn protein concentrate with zein, and adjust the pH value to 7.5-9.5 after treating at 100° C. for 10 minutes;
[0027] (2) Add Luo Han Guo protease to keep the enzyme concentration at 500U / g and keep the protein at 45-65°C for enzymolysis reaction. At the same time, continuously add sodium hydroxide concentrated solution to keep the pH value of the protein concentrated solution unchanged, and react for 120 minutes;
[0028] All the other are with embodiment 1.
Embodiment 3
[0030] Use casein or egg protein to prepare a protein concentration of 3%, and the added mogrosin protease has an enzyme concentration of 1000U / g, and the rest are the same as in Example 1 or Example 2.
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