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Alterneria tenuissima and application thereof in biological weeding

A technology of Alternaria tennatus and strains, applied in the field of ecology, can solve the problems of aggravated pollution, reduced and enhanced drug efficacy, and achieve the effects of less environmental negative effects, low residual environment, and less impact on crops

Inactive Publication Date: 2016-08-17
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, 324 biotypes of 188 species of weeds have been found to be resistant to 19 types of chemical herbicides in the world, resulting in reduced efficacy, increased dosage, increased cost, and increased pollution
The result is the simplification of herbicide varieties, which also enhances the market's demand for the development of broad-spectrum, high-efficiency, low-toxicity, and safe biological herbicides for non-target organisms and mammals and the development of biological herbicide technology

Method used

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  • Alterneria tenuissima and application thereof in biological weeding
  • Alterneria tenuissima and application thereof in biological weeding
  • Alterneria tenuissima and application thereof in biological weeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and Identification of Bacteria

[0026] 1. Isolation and purification of bacteria

[0027] Referring to the isolation method of relevant pathogenic fungi in "Methods of Plant Disease Research" (edited by Fang Zhongda, China Agricultural Press, 2007.4), the diseased leaves of Geranium plants collected from Huzhu were separated and purified according to the tissue separation method, and after slant culture Store in refrigerator at 4°C. The isolated strain was named HZ-1 according to the place where it was collected.

[0028] 2. Identification

[0029] The strain HZ-1 was cultured on a PDA plate medium, and the colony growth rate, colony shape and color changes were observed regularly, and the mycelium and spore morphology were observed under an optical microscope. Combined with "Handbook of Fungal Identification" (edited by Wei Jingchao, Shanghai Science and Technology Press, 1979.9) for preliminary identification.

[0030] The morphology and physi...

Embodiment 2

[0040]Example 2 Effect of bacterial strain HZ-1 on the pathogenicity of broad-leaved weeds

[0041] Transplant quinoa, mallow, and Polygonum sorrel with normal leaf color at the 4-5 leaf stage in the field, and pig scallops at the 4-6 whorled leaf stage in flowerpots (Φ=15cm), and cultivate them in the laboratory. ; After the biocontrol fungus was expanded and cultivated for 120h in the PDB nutrient solution, 10 7 pcs mL -1 The fermented liquid was sprayed and inoculated on the transplanted healthy weed plants, and the inoculation amount was 30mL / pot. The plants inoculated with PDB culture solution were used as blank control; three repetitions were set. The inoculated weed plants were placed at room temperature, and the relative humidity was maintained at 60% with a JBX-3.5 centrifugal industrial humidifier, and the light and dark were alternately cultivated for 12 hours. After 7 days, the weed incidence degree was investigated, the fresh weight was weighed, and the inciden...

Embodiment 3

[0046] The impact of embodiment 3 strain HZ-1 on the safety of crops

[0047] Five main crops in Qinghai Province: spring wheat (Qingqing 587), spring rape (Qingza No. 5), broad bean (Qinghai No. 9), pea (Grassland 224), and highland barley (Beiqing No. 8) were planted in Φ=15cm In flowerpots, cultivated indoors for later use. After dilution of the HZ-1 fermentation broth, inoculate it on the crop plants at the 3-4 leaf stage according to the above method, set up 3 repetitions, observe the disease of the tested crops 7 days after inoculation, and record the degree of disease.

[0048] Such as Figure 5 As shown, the fermentation broth of HZ-1 strain had no pathogenicity to spring wheat (Qingqing 587), pea (Grassland 224), broad bean (Qinghai No. 9), and highland barley (Beiqing No. 8) after inoculation. Compared with the control plants, they were not affected, showing no reaction (NS); they were mildly pathogenic to spring rape (Qingza No. 5), and 3 days after inoculation, 5...

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Abstract

The invention discloses an Alterneria tenuissima strain and application thereof in biological weeding. The classified name of the strain is Alterneria tenuissima, the complete name of the strain is Alterneria tenuissima HZ-1, the strain is preserved in the China General Microbiological Culture Collection Center located at the No.3 room, No.1 yard, Beichen west road, Chaoyang district, Beijing city, China on January 11, 2016, and the preservation number is CGMCC No.11992. The Alterneria tenuissima is used for biological weeding of broad-leaved weeds such as galium tricorne, chenopodium album and malva verticillata in a highland barley field, a broad bean field and a pea field and can effectively control and prevent the broad-leaved weeds.

Description

technical field [0001] The application belongs to the field of ecology, and in particular relates to a strain of Alternaria tennatus and its application in biological weeding. Background technique [0002] Weed damage is one of the most important factors in reducing crop yields, costing global agriculture $95 billion annually. Today, weed control in farmland is still dominated by chemical herbicides. With the widespread use of chemical herbicides, the negative effects are becoming more and more obvious. With the improvement of the level of agricultural modernization, the use of herbicides will continue to increase globally. Extensive application of chemical herbicides not only brings environmental pollution crisis, but also leads to crop yield reduction and even land degradation. More than 100 chemical herbicides have been banned or canceled in more than 30 countries around the world. In addition, 324 biotypes of 188 species of weeds have been found to be resistant to 19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P13/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 郭青云朱海霞程亮李玮翁华魏有海郭良芝
Owner QINGHAI UNIVERSITY
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