45 results about "Holotrichia parallela" patented technology

The invention relates to the preparation field of sustained-release preparations, in particular to a pasty Holotrichia parallela sex attractant sustained-release preparation and a preparation method thereof. The pasty Holotrichia parallela sex attractant sustained-release preparation is prepared from sex attractant effective constituent, an auxiliary material A, an auxiliary material B, an auxiliary material C and an auxiliary material D in certain proportion, wherein the sex attractant effective constituent and the auxiliary materials A, B and C are solid, and the auxiliary material D is liquid. Compared with an existing sex attractant preparation, the sustained-release preparation has the advantages of simple preparation process, reliability in dosage preservation, and obvious release rule, and environment-friendly effect; the release curve of the sustained-release preparation meets the quadratic trinomial rule, and the pasty Holotrichia parallela sex attractant sustained-release preparation has trapping effect up to 30 days on Holotrichia parallela, overcomes the disadvantages of short lasting period and wasted labor cost of an existing dosage form, has feasibility for large-scale production, facilitates large-scale promotion in farmers and has obvious economic significance and ecological significance.

The invention discloses a Bacillus thuringiensis cry8H gene, protein and application to kill Coleoptera pest in the biological prevention technical domain, wherein the nucleotide sequence of high-toxicity cry8H gene of Coleoptera pest is displayed as SEQ ID NO1; the nucleotide sequence of the expressed cry8H gene synthesized by artificial design is displayed as SEQ ID NO3, which has the property to resist Holotrichia oblita, Anomala corpulenta and Holotrichia parallela.

The invention relates to a Bacillus thuringiensis vip1like1 / vip2like1 gene combination and application thereof, belonging to the technical field of biological control.Vip1like1 and vip2like1 genes are cloned from the initial strain HBF-18, and the sequences are respectively SEQ ID NO 1 and SEQ ID NO 2. The invention detects that synergistic actions respectively exist between the vip1like1 and vip2like1 genes, and among cry8Ga1, vip1like1 and vip2like1 genes, and the Bacillus thuringiensis vip1like1 / vip2like1 gene combination has high virulence on Coleoptera pests and has especially good disinsection effect on Holotrichia parallela. The gene combination has very important meanings for biological control of main underground pest grubs and green and sustainable development of agriculture in China, and further provides a gene source for developing anti-grub Bt engineering strains and transgenic crops.

The invention discloses a method for preventing the holotrichia parallela by using a sex attractant. When the adult of the holotrichia parallela come up out of the ground, and the sex attractant is used in four phases, wherein the ratio of L-isoleucinemethyl ester and R-linalool which are the active components of the sex attractant is 6:1 to 12:1 in the first stage, 4:1 to 10: 1 in the second stage, 2:1 to 8:1 in the third stage and:2:1 to 4: 1 in the fourth stage. The ratio of the components of the sex attractant more conforms to the actual condition of the holotrichia parallela group in the fields of most regions of the northern part of China, the utilization rate of the sex attractant is improved, the cost is lowered, the sex attractant has an obvious effect, the problem that the attracting effect of the sex attractant is poor in the later stage of the mating period of the holotrichia parallela is solved, and the damage of the holotrichia parallela to the crops in the field is effectively controlled and reduced.

The invention relates to a gel-like holotrichia parallela sex attractant slow release dosage form. The gel-like holotrichia parallela sex attractant slow release dosage form is mainly prepared from the following raw materials: a sex attractant effective ingredient, a slow release gel carrier and a solvent, wherein a mass ratio of the sex attractant effective ingredient to solid content of the slow release gel carrier is 1:(0.2-0.6); a massic volume ratio of the solid content of the slow release gel carrier to the solvent is 1:(5-20); the sex attractant effective ingredient is methyl L-isoleucinate and (R)-(-)-linalool in a mass ratio of (5-7):1; the slow release gel carrier is hydroxypropyl methylcellulose and hydroxypropyl methylcellulose phthalate in a mass ratio of 1:(0.1-0.4); and the solvent is ethanol and deionized water in a volume ratio of 1:(10-20). The slow release dosage form provided by the invention has lasting period as long as 60 days, is simple in preparation technology, obvious in release rules, green and environment-friendly, has the characteristic that the whole adult active stage of holotrichia parallela can be covered after the dosage form is thrown for one time, is beneficial to large-area popularization and has obvious economic benefit and ecological significance.

The invention provides a holotrichia parallela sex pheromone synergist and a preparation method and belongs to the technical field of comprehensive prevention and control of holotrichia parallela. The holotrichia parallela sex pheromone synergist is characterized by being prepared from holotrichia parallela sex pheromone, a synergist and an organic solvent. The holotrichia parallela sex pheromone is prepared from L-isoleucine methyl ester, (R)-(-)-linalool and 1-aminocyclohexanecarboxylic acid, the synergist is prepared from cis-9, trans-11-tetradecadien acetate, cis-5, trans-7-dodecadien acetate, honey and mustard oil, and the organic solvent is prepared from dichloroethane, cyclohexane and fatty acid methyl ester. The synergist has the advantages of being sensitive, specific, high in efficiency, long in lasting period, biodegradable, good in biological compatibility, free of environmental pollution, low in cost and the like, can be used for population monitoring of the holotrichia parallela, prevention and control of trapping and killing of a large number of holotrichia parallela and accurate prediction of the density of a next generation of insect populations and has very good economic benefits and ecological benefits.