45 results about "Holotrichia parallela" patented technology

The invention discloses a Bacillus thuringiensis cry8H gene, protein and application to kill Coleoptera pest in the biological prevention technical domain, wherein the nucleotide sequence of high-toxicity cry8H gene of Coleoptera pest is displayed as SEQ ID NO1; the nucleotide sequence of the expressed cry8H gene synthesized by artificial design is displayed as SEQ ID NO3, which has the property to resist Holotrichia oblita, Anomala corpulenta and Holotrichia parallela.

The invention provides metarhizium anisopliae MAYX130921 and an application thereof. The metarhizium anisopliae MAYX130921 is preserved on Dec 4th, 2014 in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10139. The metarhizium anisopliae MAYX130921 is a bacterial strain initially separated from holotrichia parallela larvas, is easy to culture and can grow rapidly, sporulation quantity is high, and spore germination rate is high; meanwhile, conidiospores of the metarhizium anisopliae MAYX130921 have strong pathogenicity on the holotrichia parallela larvas, are environment-friendly and hardly produce drug resistance and can be widely applied to prevention and control of holotrichia parallela.

The invention discloses an acetic acid cis-3-hexenyl ester synergistic holotrichia parallela sex attractant, wherein the weight ratio of the sex attractant and acetic acid cis-3-hexenyl ester is (3-7):1. The sex attractant comprises a holotrichia parallela sex pheromone component and an organic solvent, wherein the holotrichia parallela sex pheromone component comprises methyl L-isoleucinate, (R)-(-)-linalool and 1-aminocyclohexanecarboxylic acid in a weight ratio of 6: 1: 0.1 and the weight ratio of the holotrichia parallela sex pheromone component and the organic solvent is 1: 3. The organic solvent is one or more of dichloromethane, methanol or acetone. Behavior experiments show that EAG and selective reaction rate of male and female worms on a mixture of the sex attractant and acetic acid cis-3-hexenyl ester are relatively good. The field trapping experiments on holotrichia parallela show that acetic acid cis-3-hexenyl ester has a synergistic effect on the trapping effect of the sex attractant, thereby providing a ground for monitoring and preventing and treating holotrichia parallela by using plant volatile matters and insect sex pheromone in a synergistic manner.

The invention relates to bacillus thuringiensis gene combination and engineering bacteria and a preparation method thereof and belongs to the field of biological prevention and control. The gene combination of cry8Eal, cry8Ca2 and cry3Aa7 has a synergistic effect between every two or among the three genes, particularly a good effect for the synergy between cry3Aa7 and cry8Eal as well as the synergy among the genes cry8Eal, cry8Ca2 and cry3Aa7. Engineering bacteria BIOT1853A thus constructed containing three genes are poisonous to holotrichia parallela, anomala corpulenta, potato beetles, colaphellus bowringi and other coleopter multiple pests at the same time, and in addition, maybe have higher poisonousness for several pests. The engineering bacteria BIOT1853A obtained through screening by allele homologous recombination are free of antibiotic genes, thereby enhancing the bio-safety of transgenic engineering bacteria.

The invention relates to the preparation field of sustained-release preparations, in particular to a pasty Holotrichia parallela sex attractant sustained-release preparation and a preparation method thereof. The pasty Holotrichia parallela sex attractant sustained-release preparation is prepared from sex attractant effective constituent, an auxiliary material A, an auxiliary material B, an auxiliary material C and an auxiliary material D in certain proportion, wherein the sex attractant effective constituent and the auxiliary materials A, B and C are solid, and the auxiliary material D is liquid. Compared with an existing sex attractant preparation, the sustained-release preparation has the advantages of simple preparation process, reliability in dosage preservation, and obvious release rule, and environment-friendly effect; the release curve of the sustained-release preparation meets the quadratic trinomial rule, and the pasty Holotrichia parallela sex attractant sustained-release preparation has trapping effect up to 30 days on Holotrichia parallela, overcomes the disadvantages of short lasting period and wasted labor cost of an existing dosage form, has feasibility for large-scale production, facilitates large-scale promotion in farmers and has obvious economic significance and ecological significance.

The invention relates to a new bacterial strain of Bacillus thuringiensis for killing grub pest and a pest killing protein thereof. The new bacterial strain has the collection number of CCTCC (China Center For Type Culture Collection) No. M2011267. The bacterial strain and the pest killing protein provided by the invention can be used for preparing the preparation capable of preventing and controlling grub pests. The bacterial strain provided by the invention has specific insecticidal activity on the grub pests. The pest killing crystal protein provided by the invention has good prevention and control effect on main soil insects such as Holotrichia oblita and Holotrichia parallela in China.

The invention relates to a slow-releasing sex attractant and a preparation method thereof and particularly relates to a granular holotrichia parallela slow-releasing sex attractant and a preparation method thereof. The granular holotrichia parallela slow-releasing sex attractant is prepared from sex attractant effective components, a slow-releasing carrier, a diluting agent, an auxiliary agent and a wetting agent according to a certain ratio, wherein the sex attractant effective components comprise methyl L-isoleucinate and (R)-(-)-linalool; the slow-releasing carrier is medical starch. Compared with an existing holotrichia parallela sex attractant, the granular holotrichia parallela slow-releasing sex attractant has the advantages of being simple in preparation process, reliable in dosage preservation, obvious in releasing rule and the like, and is absolutely environmentally-friendly; a releasing curve of a slow-releasing preparation meets a quadratic trinomial rule; the attraction acting effect on holotrichia parallela can last for 15-20 days and the disadvantages that the lasting period is short and the labor cost is wasted in an existing preparation are overcome; the frequent raining characteristic in a rain spell is avoided; the granular holotrichia parallela slow-releasing sex attractant has the feasibility of large-scale production, is good for large-scale popularization and has the economic significance and the ecological significance.

The invention discloses a method for preventing the holotrichia parallela by using a sex attractant. When the adult of the holotrichia parallela come up out of the ground, and the sex attractant is used in four phases, wherein the ratio of L-isoleucinemethyl ester and R-linalool which are the active components of the sex attractant is 6:1 to 12:1 in the first stage, 4:1 to 10: 1 in the second stage, 2:1 to 8:1 in the third stage and:2:1 to 4: 1 in the fourth stage. The ratio of the components of the sex attractant more conforms to the actual condition of the holotrichia parallela group in the fields of most regions of the northern part of China, the utilization rate of the sex attractant is improved, the cost is lowered, the sex attractant has an obvious effect, the problem that the attracting effect of the sex attractant is poor in the later stage of the mating period of the holotrichia parallela is solved, and the damage of the holotrichia parallela to the crops in the field is effectively controlled and reduced.

The invention discloses a Holotrichia parallela attractant, which contains an effective amount of anisole. The invention also discloses a purpose of the anisole as Holotrichia parallela attractant and a Holotrichia parallela trap. A field trapping test proves that the invention has a good attraction effect for Holotrichia parallela, and the number of the insects attracted by using anisole is obvious higher than that attracted by dibutyl phthalate, geraniol, eugenol and other attractants. The attractant not only can attract female adult Holotrichia parallela, but also can attract male Holotrichia parallela, and has the advantages of simple manufacture, environmental protection without pollution and damage to natural enemy and beneficial insect, crop safety and good application prospect.

The invention discloses a method for monitoring holotrichia parallela by utilizing a sex pheromone, comprising the following steps: placing a holotrichia parallela sex lure into each trapper; placing the trappers in the field according to a certain distance before the holotrichia parallela overwinters to adult and comes up out of the soil; surveying the quantity of the holotrichia parallela collected by sex lures in the trappers; and recording the quantity of the test insects. The method provided by the invention solves the problem of inaccurate holotrichia parallela monitoring methods, trouble and lag and the like in the current production, and has the advantages of being simple and convenient in operation and accurate in monitoring and possessing the capability of objectively reflectingthe regularity of occurrence and development of the holotrichia parallela and the like.

A method for improving the attractive effect of a Holotrichia parallela sex attractant is characterized in that the sex attractant is combined with a food attractant, a light attractant and a shape attractant to attract male Holotrichia parallela in a synergistic manner, so defects of single attractants are avoided, the attractive effect is greatly improved, and Holotrichia parallela in peanut fields is well controlled.

The invention relates to a method for establishing an agrobacterium rhizogene mediated peanut root inducing system, and application thereof, belonging to the field of biotechnology. According to the invention, the peanut root system is induced by using agrobacterium rhizogene and a microinjection method to obtain a chimeric plant; simultaneously, the method of the system is optimized, i.e. the peanut is infected by performing microinjection on the epicotyl as an explant; the highest conversion efficiency is 61%; and simultaneously, the bioactivities of cry8Ea1 and cry8Ha1 genes optimized by codon on the larvae of holotrichia parallela are verified. The invention successfully establishes a peanut root inducing system, provides a quick verification method for inducing a peanut plant from an extraneous gene, and provides a technical support for further acquiring genetically modified peanut plants.

The invention relates to a Bacillus thuringiensis vip1like1 / vip2like1 gene combination and application thereof, belonging to the technical field of biological control.Vip1like1 and vip2like1 genes are cloned from the initial strain HBF-18, and the sequences are respectively SEQ ID NO 1 and SEQ ID NO 2. The invention detects that synergistic actions respectively exist between the vip1like1 and vip2like1 genes, and among cry8Ga1, vip1like1 and vip2like1 genes, and the Bacillus thuringiensis vip1like1 / vip2like1 gene combination has high virulence on Coleoptera pests and has especially good disinsection effect on Holotrichia parallela. The gene combination has very important meanings for biological control of main underground pest grubs and green and sustainable development of agriculture in China, and further provides a gene source for developing anti-grub Bt engineering strains and transgenic crops.

The invention relates to a gel-like holotrichia parallela sex attractant slow release dosage form. The gel-like holotrichia parallela sex attractant slow release dosage form is mainly prepared from the following raw materials: a sex attractant effective ingredient, a slow release gel carrier and a solvent, wherein a mass ratio of the sex attractant effective ingredient to solid content of the slow release gel carrier is 1:(0.2-0.6); a massic volume ratio of the solid content of the slow release gel carrier to the solvent is 1:(5-20); the sex attractant effective ingredient is methyl L-isoleucinate and (R)-(-)-linalool in a mass ratio of (5-7):1; the slow release gel carrier is hydroxypropyl methylcellulose and hydroxypropyl methylcellulose phthalate in a mass ratio of 1:(0.1-0.4); and the solvent is ethanol and deionized water in a volume ratio of 1:(10-20). The slow release dosage form provided by the invention has lasting period as long as 60 days, is simple in preparation technology, obvious in release rules, green and environment-friendly, has the characteristic that the whole adult active stage of holotrichia parallela can be covered after the dosage form is thrown for one time, is beneficial to large-area popularization and has obvious economic benefit and ecological significance.

The invention discloses a preparation method for extracting chitosan from holotrichia parallela, which comprises the following steps: rinsing, freezing, drying and smashing the holotrichia parallela to remove inorganic salt and protein of the holotrichia parallela, subsequently, soaking the obtained holotrichia parallela in concentrated alkaline for reaction at a specific temperature for certain time; and finally, filtering, washing, drying and extracting the reaction product to prepare chitosan. According to the method, the agricultural pest is taken as the raw material to implement the reutilization of biological resources; the acid treatment quantity and time during extraction are greatly reduced and the extraction cost is also reduced; and furthermore, less waste liquor is generated and less pollution is caused to the environment in the technical process; and the prepared chitosan has high quality and is relatively low in contents of ash, heavy metal and calcium.

The invention relates to bacillus thuringiensis engineering bacteria and application thereof, and belongs to the field of biological control. A shuttle vector pSTK-8G containing a cry8Gal gene is led to a bacterial strain BIOT185, so as to obtain a recombination strain BIOT1858G. The recombinant strain BIOT1858G has toxicity on coleopteran multiple pest holotrichia parallela, anomala corpulenta, holotrichia oblita and the like; and the toxicity on a plurality of insect pests is also improved.

The invention provides a holotrichia parallela adult and larval artificial breeding method. The holotrichia parallela adult artificial breeding method comprises spreading breeding soil inside an adultbreeding box; mixed-stocking male and female holotrichia parallela adults inside the adult breeding box; adjusting the temperature, humidity and light of the adult breeding environment; feeding the male and female holotrichia parallela. Preferably, the holotrichia parallela is holotrichia oblita.

The invention relates to the preparation field of sustained-release preparations, in particular to a pasty Holotrichia parallela sex attractant sustained-release preparation and a preparation method thereof. The pasty Holotrichia parallela sex attractant sustained-release preparation is prepared from sex attractant effective constituent, an auxiliary material A, an auxiliary material B, an auxiliary material C and an auxiliary material D in certain proportion, wherein the sex attractant effective constituent and the auxiliary materials A, B and C are solid, and the auxiliary material D is liquid. Compared with an existing sex attractant preparation, the sustained-release preparation has the advantages of simple preparation process, reliability in dosage preservation, and obvious release rule, and environment-friendly effect; the release curve of the sustained-release preparation meets the quadratic trinomial rule, and the pasty Holotrichia parallela sex attractant sustained-release preparation has trapping effect up to 30 days on Holotrichia parallela, overcomes the disadvantages of short lasting period and wasted labor cost of an existing dosage form, has feasibility for large-scale production, facilitates large-scale promotion in farmers and has obvious economic significance and ecological significance.

The invention provides an active component of a Holotrichia parallela trapping agent. The active component of the Holotrichia parallela trapping agent comprises at least one of trans-2-hexenal, methyl salicylate, cis-3-hexenyl acetate, n-hexyl alcohol and linalool. The invention also provides a Holotrichia parallela trapping agent which comprises the active component and a non-polar organic solvent. The invention also provides a method for trapping the Holotrichia diomphalia. The method comprises the following steps: preparing the Holotrichia diomphalia trapping agent; adding the Holotrichia diomphalia trapping agent into a brown glass bottle with a child-mother plug to prepare a Holotrichia diomphalia trapping agent lure; hanging the basin trap on which the Holotrichia parallela trapping agent lure is hung on the branches of the fruit tree; washing powder water is added into the basin trap, then the child-mother plug is opened, the Holotrichia parallela trapping agent is released through the release hole in the child-mother plug, and then the Holotrichia parallela is trapped. The Holotrichia parallela trapping agent provided by the invention is convenient to use, efficient, non-toxic, pollution-free, harmless to natural enemies, low in cost and long in period of validity.

The invention discloses a Bacillus thuringiensis cry8H gene, protein and application to kill Coleoptera pest in the biological prevention technical domain, wherein the nucleotide sequence of high-toxicity cry8H gene of Coleoptera pest is displayed as SEQ ID NO1; the nucleotide sequence of the expressed cry8H gene synthesized by artificial design is displayed as SEQ ID NO3, which has the property to resist Holotrichia oblita, Anomala corpulenta and Holotrichia parallela.

The invention provides a holotrichia parallela sex pheromone synergist and a preparation method and belongs to the technical field of comprehensive prevention and control of holotrichia parallela. The holotrichia parallela sex pheromone synergist is characterized by being prepared from holotrichia parallela sex pheromone, a synergist and an organic solvent. The holotrichia parallela sex pheromone is prepared from L-isoleucine methyl ester, (R)-(-)-linalool and 1-aminocyclohexanecarboxylic acid, the synergist is prepared from cis-9, trans-11-tetradecadien acetate, cis-5, trans-7-dodecadien acetate, honey and mustard oil, and the organic solvent is prepared from dichloroethane, cyclohexane and fatty acid methyl ester. The synergist has the advantages of being sensitive, specific, high in efficiency, long in lasting period, biodegradable, good in biological compatibility, free of environmental pollution, low in cost and the like, can be used for population monitoring of the holotrichia parallela, prevention and control of trapping and killing of a large number of holotrichia parallela and accurate prediction of the density of a next generation of insect populations and has very good economic benefits and ecological benefits.

The invention discloses a method for monitoring holotrichia parallela by utilizing a sex pheromone, comprising the following steps: placing a holotrichia parallela sex lure into each trapper; placing the trappers in the field according to a certain distance before the holotrichia parallela overwinters to adult and comes up out of the soil; surveying the quantity of the holotrichia parallela collected by sex lures in the trappers; and recording the quantity of the test insects. The method provided by the invention solves the problem of inaccurate holotrichia parallela monitoring methods, trouble and lag and the like in the current production, and has the advantages of being simple and convenient in operation and accurate in monitoring and possessing the capability of objectively reflecting the regularity of occurrence and development of the holotrichia parallela and the like.

The invention relates to a gel-like holotrichia parallela sex attractant slow release dosage form. The gel-like holotrichia parallela sex attractant slow release dosage form is mainly prepared from the following raw materials: a sex attractant effective ingredient, a slow release gel carrier and a solvent, wherein a mass ratio of the sex attractant effective ingredient to solid content of the slow release gel carrier is 1:(0.2-0.6); a massic volume ratio of the solid content of the slow release gel carrier to the solvent is 1:(5-20); the sex attractant effective ingredient is methyl L-isoleucinate and (R)-(-)-linalool in a mass ratio of (5-7):1; the slow release gel carrier is hydroxypropyl methylcellulose and hydroxypropyl methylcellulose phthalate in a mass ratio of 1:(0.1-0.4); and the solvent is ethanol and deionized water in a volume ratio of 1:(10-20). The slow release dosage form provided by the invention has lasting period as long as 60 days, is simple in preparation technology, obvious in release rules, green and environment-friendly, has the characteristic that the whole adult active stage of holotrichia parallela can be covered after the dosage form is thrown for one time, is beneficial to large-area popularization and has obvious economic benefit and ecological significance.

The invention discloses a Holotrichia parallela attractant, which contains an effective amount of anisole. The invention also discloses a purpose of the anisole as Holotrichia parallela attractant and a Holotrichia parallela trap. A field trapping test proves that the invention has a good attraction effect for Holotrichia parallela, and the number of the insects attracted by using anisole is obvious higher than that attracted by dibutyl phthalate, geraniol, eugenol and other attractants. The attractant not only can attract female adult Holotrichia parallela, but also can attract male Holotrichia parallela, and has the advantages of simple manufacture, environmental protection without pollution and damage to natural enemy and beneficial insect, crop safety and good application prospect.

The invention relates to bacillus thuringiensis engineering bacteria and application thereof, and belongs to the field of biological control. A shuttle vector pSTK-8G containing a cry8Gal gene is led to a bacterial strain BIOT185, so as to obtain a recombination strain BIOT1858G. The recombinant strain BIOT1858G has toxicity on coleopteran multiple pest holotrichia parallela, anomala corpulenta, holotrichia oblita and the like; and the toxicity on a plurality of insect pests is also improved.

The invention relates to a content analysis method for a Holotrichia parallela slow release sex attractant preparation. The method includes the steps of: 1. single component content determination; 2. composite component content determination; 3. content determination of composite components in preparation; and 4. result analysis: the high molecular material accessories contained in the Holotrichia parallela sex attractant preparation does not influence the content determination of composite components. The invention uses a simple ultraviolet analysis method, and compared with existing sex attractant preparation gas chromatography content method, the method provided by the invention has the advantages of convenient instrument use, simple analysis steps, analysis cost saving, stable and reliable data during large dose use of sex attractant, is suitable for large-scale sex attractant analysis, has obvious economic significance, and adopts a 90-100% ethanol solution as the analytical extraction solvent, which has the advantages of environmental protection, low toxicity, and low price, etc.

The invention provides a holotrichia parallela sex pheromone synergist and a preparation method and belongs to the technical field of comprehensive prevention and control of holotrichia parallela. The holotrichia parallela sex pheromone synergist is characterized by being prepared from holotrichia parallela sex pheromone, a synergist and an organic solvent. The holotrichia parallela sex pheromone is prepared from L-isoleucine methyl ester, (R)-(-)-linalool and 1-aminocyclohexanecarboxylic acid, the synergist is prepared from cis-9, trans-11-tetradecadien acetate, cis-5, trans-7-dodecadien acetate, honey and mustard oil, and the organic solvent is prepared from dichloroethane, cyclohexane and fatty acid methyl ester. The synergist has the advantages of being sensitive, specific, high in efficiency, long in lasting period, biodegradable, good in biological compatibility, free of environmental pollution, low in cost and the like, can be used for population monitoring of the holotrichia parallela, prevention and control of trapping and killing of a large number of holotrichia parallela and accurate prediction of the density of a next generation of insect populations and has very good economic benefits and ecological benefits.

The invention relates to a bifenthrin-containing pharmaceutical composition, and belongs to the field of chemical prevention and control, wherein the bifenthrin-containing pharmaceutical composition comprises an effective component bifenthrin, a triazole fungicide or a methoxy acrylate fungicide and an auxiliary agent thereof, can provide good synergistic effect in the prevention and control of underground pests, especially Grub, and the indoor biological measurement results of the bifenthrin-containing pharmaceutical composition show that the synergistic ratio of the bifenthrin-containing pharmaceutical composition in the prevention and control of Holotrichia parallela by is 80:1-1:20. According to the present invention, the bifenthrin-containing pharmaceutical composition has characteristics of remarkable synergistic effect, good prevention effect, low dosage, safety to environment and target organisms, control effects on multiple pests and the like, and can further control other pests (such as wireworm, cutworm and the like) and crop diseases.