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A method for content analysis of a black beetle sex pheromone preparation

The invention relates to a technology for the analysis method and application in the field of content analysis of the slow-release inducer of the beetle, can solve the problems of high analysis cost, large content calculation error, small molecular weight and the like, and achieves obvious economic significance, stable and reliable data, Analyze the effect of simple steps

Inactive Publication Date: 2017-03-22
QINGDAO UNIV OF SCI & TECH
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Problems solved by technology

However, the sex pheromone component of the black beetle is a compound component with volatile properties, small molecular weight and strong volatility. The original analysis method of this component is gas chromatography. The analysis method has the advantage of high analysis accuracy, but there are conditions for pheromone extraction. It is cumbersome, the analysis cost is high, and there is a lot of rain during the mating period of scarab adults, the pheromone preparation is easily eroded by wind and rain, and has the disadvantage of large content calculation error, which limits the further promotion of field preparation technology

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  • A method for content analysis of a black beetle sex pheromone preparation
  • A method for content analysis of a black beetle sex pheromone preparation

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Embodiment Construction

[0017] A method for analyzing the content of a slow-release attractant for black gill beetles, comprising the following steps:

[0018] 1. Determination of single-component content: place the slow-release sex attractant of the black gill beetle at -18°C, so that the active ingredients of the sex attractant exist in the solid component and the liquid component, respectively take the solid component and the liquid component, and respectively Add ethanol solution to the solid and liquid components to dissolve them, and scan with an ultraviolet analyzer. There are ultraviolet maximum absorptions at 210nm and 212nm wavelengths respectively, and the solid part is 0.1-1.0mg·mL -1 There is a linear relationship within the concentration range, r=0.9998; the liquid part has a concentration of 0.02~0.16mg·mL -1 There is a linear relationship in it, r=0.9999;

[0019] 2. Determination of the content of the composite component: take the solid part and the liquid part to form the composite...

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Abstract

The invention relates to a content analysis method for a Holotrichia parallela slow release sex attractant preparation. The method includes the steps of: 1. single component content determination; 2. composite component content determination; 3. content determination of composite components in preparation; and 4. result analysis: the high molecular material accessories contained in the Holotrichia parallela sex attractant preparation does not influence the content determination of composite components. The invention uses a simple ultraviolet analysis method, and compared with existing sex attractant preparation gas chromatography content method, the method provided by the invention has the advantages of convenient instrument use, simple analysis steps, analysis cost saving, stable and reliable data during large dose use of sex attractant, is suitable for large-scale sex attractant analysis, has obvious economic significance, and adopts a 90-100% ethanol solution as the analytical extraction solvent, which has the advantages of environmental protection, low toxicity, and low price, etc.

Description

technical field [0001] The invention relates to a method for analyzing the content of a pest attractant, in particular to a method for analyzing the content of a slow-release attractant for black beetles. Background technique [0002] In recent years, pests and diseases of peanuts, soybeans, sweet potatoes, corn and other crops have increased. Among them, the black beetle is one of the most harmful pests. Its larvae grubs do damage underground, and the adults feed on the leaves of crops and forest trees, often causing large-scale peanut failures. Therefore, the prevention and control of this pest has always been the focus and difficulty of pest control work. . At present, adults of black gill beetles are used to release sex pheromones when they are unearthed to mate at night, and artificially release pheromone preparations of beetles themselves to trap and kill adults. However, the sex pheromone component of the black beetle is a compound component with volatile properties...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
Inventor 金青曲明静李晓赵文英谭心舜叶庆国
Owner QINGDAO UNIV OF SCI & TECH
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