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Bacillus thuringiensis vip1like1/vip2like1 gene combination and application thereof

A technology of gene and composition, applied in the field of biological control

Active Publication Date: 2013-07-31
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the expression product of the cry8Ga gene is quite different from that of the starting strain (HBF-18), and the results show that the activity of the amorphic mutant strain containing the cry8Ga gene against the large black beetle and the dark beetle is lower than that of the starting strain , whose LC 50 50 times and 70 times higher respectively (Shu et al.2009) (Table 1-1)

Method used

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  • Bacillus thuringiensis vip1like1/vip2like1 gene combination and application thereof
  • Bacillus thuringiensis vip1like1/vip2like1 gene combination and application thereof
  • Bacillus thuringiensis vip1like1/vip2like1 gene combination and application thereof

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Embodiment Construction

[0063] The present invention is described in further detail below by embodiment.

[0064] 1. Experimental materials

[0065] 1.1 Strains and plasmids

[0066] The strains and plasmids used are shown in Table 2-1. The starting strains and vectors mentioned above are preserved in our laboratory and can be distributed to the public.

[0067] Table 2-1 Strains and plasmids

[0068]

[0069] 1.2 Reagents

[0070] 1.2.1 Medium

[0071] Liquid LB medium: 0.5% yeast extract, 1.0% trytone, 1.0% NaCl, pH 7.0, sterilized at 121°C for 20mim.

[0072] Solid LB medium: add 1.3% agar powder to liquid LB medium, and sterilize at 121°C for 20mim.

[0073] BH medium: 3.7% Brain and heart infusion broth, sterilized at 121°C for 20mim.

[0074] Beef extract peptone medium: 3g beef extract, 5g soybean peptone, dissolve in distilled water, adjust the pH to 7.2 with NaOH, adjust the volume to 1000mL, and sterilize at 121°C for 20min.

[0075] 1.2.2 Antibiotics

[0076] Ampicillin aqueous ...

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Abstract

The invention relates to a Bacillus thuringiensis vip1like1 / vip2like1 gene combination and application thereof, belonging to the technical field of biological control.Vip1like1 and vip2like1 genes are cloned from the initial strain HBF-18, and the sequences are respectively SEQ ID NO 1 and SEQ ID NO 2. The invention detects that synergistic actions respectively exist between the vip1like1 and vip2like1 genes, and among cry8Ga1, vip1like1 and vip2like1 genes, and the Bacillus thuringiensis vip1like1 / vip2like1 gene combination has high virulence on Coleoptera pests and has especially good disinsection effect on Holotrichia parallela. The gene combination has very important meanings for biological control of main underground pest grubs and green and sustainable development of agriculture in China, and further provides a gene source for developing anti-grub Bt engineering strains and transgenic crops.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the gene combination of bacillus thuringiensis vip1 and vip2 and the application thereof. Background technique [0002] Grubs are the general term for the larvae of the superfamily Scarabaeoidea, belonging to the order Coleoptera and the family Scarabae. There are many kinds of scarab superfamily, there are more than 30,000 kinds recorded in the world, and more than 1,800 kinds have been recorded in my country at present. This kind of pest has miscellaneous feeding habits, wide distribution, hidden life, strong adaptability, and different life history. Among them, it is harmful to agriculture, forestry and pasture. There are more than 100 species of it, and it is very difficult to prevent and treat it. The most important ones are Holotrichia parallela, Holotrichia obeita and Anomala carpulenta. [0003] The living habits of grubs are complex and diverse, and the harmf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N1/21C12N15/63C12N15/31A01N47/44A01P7/04A01H5/00C12R1/07
Inventor 束长龙张彦蕊耿丽丽张杰宋福平彭琦
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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