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Selenium-enriched microorganism preparation prepared from fermentation of mixed bacterium liquid and preparation method of selenium-enriched microorganism preparation

A technology of selenium-enriched microorganisms and mixed bacterial liquid is applied in the fields of selenium-enriched microbial preparations and preparations, and selenium-enriched nutritional microbial fertilizers, and can solve the problem of low biological activity of inorganic selenium salts, low absorption rate of inorganic selenium salts, and no antioxidant properties. and other problems, to achieve the effect of beneficial absorption, uniform particle size and cost saving

Inactive Publication Date: 2015-03-25
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But selenium exists in the form of inorganic selenium salt in this selenium-enriched preparation, and the biological activity of inorganic selenium salt is low, does not have antioxidation, and human body is very little to the absorption rate of inorganic selenium salt, and excessive consumption has certain toxicity to human body, has increased the harm to human body. Hazardous

Method used

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  • Selenium-enriched microorganism preparation prepared from fermentation of mixed bacterium liquid and preparation method of selenium-enriched microorganism preparation
  • Selenium-enriched microorganism preparation prepared from fermentation of mixed bacterium liquid and preparation method of selenium-enriched microorganism preparation
  • Selenium-enriched microorganism preparation prepared from fermentation of mixed bacterium liquid and preparation method of selenium-enriched microorganism preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A selenium-enriched microbial preparation prepared by fermentation of mixed bacterial liquid, the weight parts of raw materials are as follows:

[0053] 8 parts of sodium selenite, 5 parts of compound trace elements, 8 parts of potassium dihydrogen phosphate, 10 parts of urea, 8 parts of peptone, 4 parts of yeast powder, 8 parts of NaCl, 8 parts of NaOH, NaNO 3 4 parts, NaCl 10 parts, NH 4 Cl 4 parts, K 2 HPO 4 8 parts, 4 parts of beef extract, 10 parts of glucose, 10 parts of starch, 8 parts of calcium carbonate, 10 parts of amino acid, 2 parts of potassium nitrate, 2 parts of ferrous sulfate, 10 parts of magnesium sulfate, 25 parts of trisodium citrate, biochemical enzyme 10 parts, 850 parts of mixed bacterial solution;

[0054] The mixed bacterial liquid is prepared by the following method:

[0055] (1) Inserting the Bacillus amyloliquefaciens strain into a fermenter equipped with a fermentation broth at an inoculation amount of 0.25% by volume, and aerobically f...

Embodiment 2

[0070] As described in Example 1, the selenium-enriched microbial preparation prepared by fermentation of mixed bacterial liquid is different in that the raw material weight

[0071] The servings are as follows:

[0072] 10 parts of sodium selenite, 60 parts of chitosan hydrolyzate, 8 parts of compound trace elements, 10 parts of potassium dihydrogen phosphate, 15 parts of urea, 12 parts of peptone, 6 parts of yeast powder, 10 parts of NaCl, 10 parts of NaOH, NaNO 3 6 parts, NaCl 15 parts, NH 4 Cl 4 parts, K 2 HPO 4 12 parts, 6 parts of beef extract, 15 parts of glucose, 15 parts of starch, 12 parts of calcium carbonate, 16 parts of amino acid, 3 parts of potassium nitrate, 3 parts of ferrous sulfate, 13 parts of magnesium sulfate, 30 parts of trisodium citrate, biochemical enzyme 18 parts, 860 parts of mixed bacteria liquid.

[0073] The volume ratio of the Bacillus amyloliquefaciens solution: the Bacillus subtilis solution: the Bacillus licheniformis solution: the Bacill...

Embodiment 3

[0077] As described in Example 1, the selenium-enriched microbial preparation prepared by fermentation of mixed bacterial liquid is different in that the raw material weight

[0078] The servings are as follows:

[0079] 12 parts of sodium selenite, 70 parts of chitosan hydrolyzate, 12 parts of compound trace elements, 18 parts of potassium dihydrogen phosphate, 20 parts of urea, 18 parts of peptone, 8 parts of yeast powder, 15 parts of NaCl, 12 parts of NaOH, NaNO 3 8 parts, NaCl 20 parts, NH 4 Cl 10 parts, K 2 HPO 4 18 parts, 8 parts of beef extract, 20 parts of glucose, 20 parts of starch, 18 parts of calcium carbonate, 20 parts of amino acid, 5 parts of potassium nitrate, 5 parts of ferrous sulfate, 22 parts of magnesium sulfate, 35 parts of trisodium citrate, biochemical enzyme 25 parts, 880 parts of mixed bacterial liquid.

[0080] The volume ratio of the Bacillus amyloliquefaciens solution: the Bacillus subtilis solution: the Bacillus licheniformis solution: the Bac...

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Abstract

The invention discloses a selenium-enriched microorganism preparation prepared from fermentation of a mixed bacterium liquid. The selenium-enriched microorganism preparation consists of the following raw materials: sodium selenite, complex trace elements, monopotassium phosphate, urine, peptone powder, NaCl, NaOH, NaNO3, NaCl, NH4Cl, K2HPO4, a beef extract, glucose, starch, calcium carbonate, amino acid, potassium nitride, ferrous sulfate, magnesium sulfate, trisodium citrate, a biochemical enzyme and a mixed bacterium liquid; and the mixed bacterium liquid is a mixed liquid of the bacterium liquid of bacillus amyloliquefaciens, the bacterium liquid of bacillus subtilis, the bacterium liquid of bacillus licheniformis, the bacterium liquid of bacillus thuringiensis and the bacterium liquid of bacillus mucilaginosus. The mixed bacterium liquid applicable to the selenium-enriched microorganism preparation disclosed by the invention is conducive to microorganisms in soil, and can be used for increasing the quantity of the microorganisms in the soil and enhancing the activity of the microorganisms in the soil. Due to vital activity and metabolite, the strain is an agricultural product which can cause special fertilizer effect, growth regulating effect, effect of prevention and control of plant diseases and pests, soil bioremediation and the like to crops.

Description

technical field [0001] The invention relates to a selenium-enriched nutritional microbial fertilizer, in particular to a selenium-enriched microbial preparation prepared by fermentation of mixed bacterial liquid and a preparation method, belonging to the technical field of biological fertilizers. Background technique [0002] my country is a big selenium-deficient country. The census proves that more than 70% of the soil in our country is seriously deficient in selenium, and the selenium content in more than 95% of the grain and livestock and poultry meat in our country is less than 50 μg / kg, which belongs to a large selenium-deficient country. The per capita selenium intake in my country is only about 39 μg per day, which is less than 80% of the minimum adult requirement of 50 μg / day for success, and is far from the healthy requirement of 100-200 μg / day; it is farther away from the safe upper limit of 800 μg. In my country, except for a few areas, 90% of the population nee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05G3/04A01C21/00C12N1/20C12R1/10C12R1/125C12R1/07C05G3/60C05G3/80
CPCC05B17/00C05G3/00C05G3/60C05G3/80C05C3/00C05C5/02C05C9/00C05C11/00C05D3/02C05D5/00C05D9/02C05F1/00C05F11/08
Inventor 王曙光李晓肖岚
Owner SHANDONG UNIV
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