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Efficient bacillus thuringiensis cry8H gene, protein for vaginata destructive insect and uses of the same

A technology of Bacillus aureus and Coleoptera, which is applied to the Bacillus thuringiensis cry8H gene, protein and its application field, which is highly effective against Coleoptera pests, and can solve problems such as difficulty in achieving sustainable control

Active Publication Date: 2010-08-25
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to control the harm of grubs, comprehensive control strategies such as agriculture, chemistry, and physics are generally adopted. Although this has certain effects, it is difficult to achieve the effect of continuous control

Method used

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  • Efficient bacillus thuringiensis cry8H gene, protein for vaginata destructive insect and uses of the same
  • Efficient bacillus thuringiensis cry8H gene, protein for vaginata destructive insect and uses of the same
  • Efficient bacillus thuringiensis cry8H gene, protein for vaginata destructive insect and uses of the same

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Abstract

The invention discloses a Bacillus thuringiensis cry8H gene, protein and application to kill Coleoptera pest in the biological prevention technical domain, wherein the nucleotide sequence of high-toxicity cry8H gene of Coleoptera pest is displayed as SEQ ID NO1; the nucleotide sequence of the expressed cry8H gene synthesized by artificial design is displayed as SEQ ID NO3, which has the property to resist Holotrichia oblita, Anomala corpulenta and Holotrichia parallela.

Description

technical field The invention belongs to the technical field of biological control. The invention relates to the nucleotide sequence of cry8H gene highly toxic to coleopteran pests, to the amino acid sequence of a protein highly toxic to coleopteran pests, to artificially designed and synthesized cry8H genes that can be used in plants The nucleotide sequence of the cry8H gene expressed in , relates to the amino acid sequence of the protein encoded by the nucleotide sequence of the artificially designed cry8H gene, relates to a recombinant strain containing the cry8H gene, relates to the use of the gene to construct an expression vector, and also relates to the use of the above-mentioned gene Sequences for methods of plant transformation. Background technique Scarab belongs to Coleoptera Scarabaeidae (Scarabaeidae), and its larvae (commonly known as grubs, also referred to as "grubs" for short in the present invention hereinafter) are a class of important worldwide distributi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/31C07K14/32A01N63/02C07H21/04C12N15/82A01P7/04A01N63/23A01N63/50
CPCA01N63/00C12N15/8286A01N63/02C07K14/325C12R1/075Y02A40/146A01N63/50A01N63/23C12R2001/075C12N1/205
Inventor 束长龙宋福平王容燕张杰冯书亮黄大昉郎志宏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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