39results about How to "Inhibition of signaling pathways" patented technology

The invention belongs to the technical field of biological medicines, and discloses a biological agent for inhibiting response of cells to inflammatory factor stimulation, the biological agent comprises a PRD peptide fragment and / or a TAD peptide fragment, the amino acid sequence of the PRD peptide fragment is shown as SEQ ID NO: 1, and the amino acid sequence of the PRD peptide fragment is shownas SEQ ID NO: 2. Through a molecular biological analysis method, the PRD peptide fragment is found to be a positive regulation region for inhibiting inflammatory stimuli response in YAP1 protein, andthe TAD peptide fragment is found to be a negative regulation region for inhibiting inflammatory stimuli response in YAP1 protein; both the PRD peptide fragment and the TAD peptide fragment can inhibit signaling pathways of TNF-alpha, IL1-beta and LPS, and the PRD peptide fragment and / or the TAD peptide fragment can be used in diseases caused by excessive cytokines, so that a new way is provided for immunotherapy.

The invention provides a composition for whitening cosmetics and a preparing method thereof. This composition for whitening cosmetics is prepared from, by weight, 0.1%-0.8% of balanites roxburghii seed oil, 0.1%- 20% of pongamia glabra seed oil, 0.1%- 5% of barley seed extract, 0.1% -5%, a of magnesium ascorbyl phosphate, 0.1%-5% of arbutin and 0.1%-1.5% of protease. The composition for whitening cosmetics has the effect of balanced whitening.

The invention discloses a method for preparing the recombinant protein swHO1, extracting the total RNA of the liver tissue of rice field eel, and cloning the coding gene of rice field eel heme oxidase 1 into an expression vector through reverse transcription and PCR amplification to obtain the recombinant expression vector pET ‑HO1; transforming the recombinant expression vector pET‑HO1 into Escherichia coli BL21 (DE3) cells for expression and purification to obtain the recombinant protein swHO1. The application of a recombinant protein swHO1 in the regulation of oxidative stress induced by pathogenic bacteria and the expression regulation of immune-related genes in rice field eel. The invention constructs the prokaryotic expression vector of the eel heme oxidase 1 gene, induces the expression, and obtains the purified recombinant protein swHO1 by affinity chromatography. The rice field eel heme oxidase 1 prepared by the invention has the functions of improving the antioxidant capacity in the serum tissue treated with pathogenic bacteria and improving the expression of liver immune-related genes.

The invention discloses a GPR1 antagonistic polypeptide and a derivative and application thereof and particularly relates to a GPR1 antagonistic polypeptide shown in SEQ ID No.1-5 and a derivative thereof. The derivative of the binding peptide is a product obtained in the mode that conventional modification is conducted on a GPR1 binding peptide amino acid side chain group and an amino terminal ora carboxyl terminal of a GPR1 antagonistic polypeptide fragment or a product obtained in the mode that a label for detection or purification of polypeptide or protein is linked to the GPR1 antagonistic polypeptide. The binding peptide and the derivative thereof can be combined with GPR1 in vitro, by stopping combination of chemerin and GPR1, increase of the cAMP concentration is promoted, the (Ca2+) influx caused by chemerin is inhibited, effective micromolecule treatment drugs are provided for diseases, such as breast cancer, of high-expression GPR1 receptors, and the GPR1 antagonistic polypeptide can be widely applied in the fields of medicine and biology.

The invention discloses an STAT3 (Signal Transducer and Activator of Transcription 3)-targeting small molecular compound as well as a preparation method and application thereof. The STAT3 inhibitor is a compound shown as a general formula I in the specification, wherein R<1> is 5-chloro-2-hydroxyphenyl, (E)-3-(6-bromo-2-pyridine)-2-cyan or 4-(2-phenylquinoline); R<2> is hydrogen or C1-C6 linear alkyl or branched alkyl. The compound has the advantages that the proliferation and the survival of various tumor cells of breast cancer, lung cancer, liver cancer, cervical cancer, prostate cancer and the like can be remarkably inhibited; the STAT3 signal channel of the tumor cells is inhibited; therefore, the tumor cell apoptosis is induced and the effect of treating the tumor diseases is achieved.

The present invention provides a polypeptide inhibitor, whose amino acid sequence structure is as follows: The present invention also provides the use of the above-mentioned polypeptide inhibitor in the preparation of a drug for inhibiting LSD1 enzyme activity. The polypeptide of the invention can be covalently combined with the cysteine ​​(Cys360) near the active pocket of the LSD1 enzyme, thereby inhibiting the activity of the LSD1 protease, and can be used for anti-tumor treatment in vivo.