The invention discloses a reference gene screening method for stable expression of suaeda salsa under salt stress. According to transcriptome analysis results obtained in a laboratory and reported reference gene sequence information of other plants, fourteen reference gene candidates with relatively stable expression are preliminarily screened and named as ACT7, ACT1, CCD1, TUA5, UPL1, UBC28, EF1alpha, PP2A, DREB1D, TIM, V-H(+)-ATPase, MPK6, PHT4:5 and CESA1 respectively. For these gene candidates, corresponding amplification primers are designed and reference gene screening is conducted. Tengenes stably expressed in a gene expression profile of suaeda salsa are screened out as the reference genes of suaeda salsa under salt stress, and the stability and reliability of suaeda salsa gene expression analysis and research under salt stress conditions are easily improved. The method makes up for the defect of an existing method in which the use of a single reference gene can lead to errorsand deviations in experimental results, a more reliable conclusion can be obtained, and the method is of great significance for promoting the improvement and perfecting of molecular biological methods and revealing cell differentiation, development and morphogenesis.