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Method for detecting pollution toxicity of soil by adopting electric signals of double-chamber microbial fuel cell

A fuel cell and soil pollution technology, which is applied in the direction of measuring devices, analyzing materials, and material analysis through electromagnetic means, can solve the problems of long detection cycle, high cost, and easy pollution, and achieve short detection cycle, low cost, and The effect of low running cost

Inactive Publication Date: 2014-03-19
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0006] In view of the deficiencies and defects in the existing technology, including the problem of high cost in the soil microbial activity detection technology, and the high cost and long detection cycle of the voltage or current signal of the microbial fuel cell in the method of reflecting the water quality pollution And the problem that easily causes pollution, the object of the present invention is to provide a kind of new method of detecting pollutant toxicity in soil

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  • Method for detecting pollution toxicity of soil by adopting electric signals of double-chamber microbial fuel cell
  • Method for detecting pollution toxicity of soil by adopting electric signals of double-chamber microbial fuel cell
  • Method for detecting pollution toxicity of soil by adopting electric signals of double-chamber microbial fuel cell

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specific Embodiment approach

[0028] Specific implementation method: MFC with double chamber structure is adopted, the wall size of each chamber is 6cm×6cm×6cm, the wall thickness is 0.5cm, and the effective volume is 216mL. The two compartments are separated by a proton exchange membrane. The carbon felt is used as the electrode, the specifications of the cathode carbon felt and the anode carbon felt are 4cm×4cm×0.5cm (length×width×thickness), the distance between the electrodes is 6cm, and the external resistance of 1000Ω is connected between the electrodes. The soil is the woodland soil in the middle and lower reaches of the Yangtze River, which is a typical yellow-brown soil. Divide the soil into 5 parts and add different Cr(VI) concentrations of K 2 Cr 2 o 7 Solution and mix well with the soil, so that the Cr(VI) concentration in the soil reaches 0 (no Cr(VI) control), 50, 100, 200 and 400mgkg -1 . After the soil was aged for 1 day, electricity production was detected by MFC and dehydrogenase act...

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Abstract

The invention discloses a method for detecting the pollution toxicity of soil by adopting electric signals of a double-chamber microbial fuel cell and belongs to the technical field of detection of soil pollution. According to the method, electric quantity generated by decomposing organic matters through soil microorganisms is used as an index for detecting the pollution toxicity of the soil; compared with a traditional method for detecting the toxicity by microorganism activity, biomass and the like, the method has the advantages of low cost, easiness in operation and short detection period. The double-chamber microbial fuel cell used in the method comprises an anode, a cathode, an anode fixing groove, a cathode fixing groove, an anode chamber, a cathode chamber, a top cover of the anode chamber, a top cover of the cathode chamber, a diaphragm, a rubber cushion, a lead wire, a load, bolts and nuts. Before the double-chamber microbial fuel cell is operated, polluted soil and glucose are mixed and are added into the anode chamber; a potassium ferricyanide solution is introduced into the cathode chamber to be used as an electron acceptor; after the double-chamber microbial fuel cell is operated for 48 hours, the generated electric quantity in the 48 hours is calculated and is used for evaluating the toxicity of pollutants. The method for detecting the pollution toxicity of the soil by adopting the electric signals of the double-chamber microbial fuel cell has high scientific values and application values.

Description

technical field [0001] The invention belongs to a soil pollution toxicity detection technology; in particular, it relates to a method for detecting soil pollution toxicity by electric signals of a double-chamber soil microbial fuel cell. Background technique [0002] With the development of industry, a large number of toxic substances have entered the environment, mainly including heavy metals and organic pollutants. Many pollutants enter the soil, destroy soil health, reduce soil microbial activity, and cause soil degradation. Therefore, the detection of soil pollution toxicity is very important, usually through the detection of soil microbial activity to reflect the toxicity of pollutants and the degree of pollution. Commonly used methods to detect soil microbial activity include dehydrogenase activity detection, carbon source utilization atlas, and soil microcalorimetry. [0003] From the perspective of detection cost, the cost of dehydrogenase activity detection, carbo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403
Inventor 邓欢姜允斌钟文辉
Owner NANJING NORMAL UNIVERSITY
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