The invention discloses a nanoparticle liposome for releasing different medicines in programmed manner and preparation and use thereof. The nanoparticle liposome is mainly prepared from the following components in part by mass: 19.34 to 50.79 parts of polylactic-co-glycolic acid, 5.78to 25.73 parts of dihydroartemisinin and phospholipid composite, 0.01 to 25.27 parts of phospholipid, 0.01 to 15.38 parts of didodecyldimethylammonium bromide, 0.01 to 20.0 parts of cholesterol, and 0.01 to 10.16 parts of adriamycin, wherein the molecular weight of the polylactic-co-glycolic acid is 5,000 to 300,000, and the phospholipid is natural phospholipid or synthetic phospholipid. The nanoparticle liposome for loading medicines of different natures can be used for treating breast cancer and leukocythemia, which have resistance to adriamycin, inverse the medicine resistance of tumors, improve the utilization rate of medicines and reduce pain in patients and the medical cost of patients.