N-15 marked microcystis and production method thereof

Abstract

The invention discloses N-15 marked microcystis and a production method thereof. The production method comprises the following steps of microcystis aeruginosa cleaning: using the microcystis aeruginosa in a logarithmic phase as microcystis aeruginosa seeds, performing suspension cleaning on the microcystis aeruginosa seeds by deionized water, and collecting microcystis aeruginosa bodies after centrifugation, wherein the number of cleaning times is at least two; microcystis aeruginosa culture: inoculating the cleanly cleaned microcystis aeruginosa into a special culture medium with N-15, performing culture in light (the light intensity is about 2500 to 5000LUX, and the temperature is 25 to 28 DEG C), shaking up a culture bottle for 1 to 5 times every day, and performing culture for 10 to 30days to obtain culture liquid; and microcystis extraction: performing lysis treatment on microcystis aeruginosa cells in the culture liquid, performing centrifugation, and collecting liquid supernatant to obtain microcystis crude extraction liquid. The production method is simple; the operation is easy; the N-15 marking rate of the prepared microcystis is high; when the prepared microcystis is used as a standard product, a matrix effect is reduced; and the quantification is more accurate. Compared with the existing non-standard microcystis, better application prospects are realized.

Description

technical field [0001] The invention relates to the technical field of production methods of microcystins, in particular to an N-15 labeled microcystin and a production method thereof. Background technique [0002] Microcystis aeruginosa is the dominant species of cyanobacteria "freshwater bloom". It has strong adaptability and fast reproduction speed. The microcystis produced are mainly LR, RR, YR, etc., which are a kind of biologically active monocyclic heptapeptide, which can be enriched in aquatic zooplankton and fish , when the algae cells rupture and die, the toxin will be released directly into the water body. Humans will induce liver damage or even liver cancer and other diseases by drinking polluted water or eating aquatic animals. This requires fast, efficient and accurate extraction and detection of water and food. Microcystin content in medium to ensure a healthy diet. Therefore, it is urgent to produce high-purity, high-stability microcystin standard products,...

AI Technical Summary

Problems solved by technology

[0004] At present, the synthesis pathway of microcystin molecules in Microcystis is not clear, and the sources of C, H, and O in the molecules are complicated. If the isotopes of these three elements are used to label algal toxins, the cost is high and the process is uncontrollable. The nitrogen source in the medium is relatively single

There is no report about the isotope-labeled microcystin internal standard product and its production

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