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Photosynthetic bacteria culture medium

A technology for photosynthetic bacteria and culture medium, applied to bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of darkening of the color of bacterial liquid, high production cost, low concentration of photosynthetic bacteria growth bacteria, etc., to achieve bright colors and reduce production. Cost and effect of high bacterial concentration

Inactive Publication Date: 2009-04-15
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that there is a high concentration of yeast extract in the existing photosynthetic bacteria culture medium to make the color of the bacteria liquid dark, the production cost is high, the pH value of the culture medium will increase with the prolongation of the culture time, thereby inhibiting the growth of photosynthetic bacteria and The problem of low bacterial concentration, and provide a photosynthetic bacteria culture medium

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  • Photosynthetic bacteria culture medium

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specific Embodiment approach 1

[0009] Specific embodiment one: the photosynthetic bacteria culture medium of this embodiment is made of low molecular weight carbon-containing organic matter, ammonium salt, yeast extract, MgSO 4 , CaCl 2 、K 2 HPO 4 、KH 2 PO 4 , trace elements and distilled water; the carbon content of low molecular weight carbon-containing organic matter per 1L of photosynthetic bacteria culture medium is 0.06-4.00g / L, the nitrogen content of ammonium salt is 0.07-2.90g / L, and the content of yeast extract 0.10~1.00g / L, MgSO 4 The content is 0.10~0.14g / L, CaCl 2 The content is 0.73~0.77g / L, K 2 HPO 4 The content is 0.48~0.52g / L, KH 2 PO 4 Distilled water with content of 0.28~0.32g / L, trace element content of 0.80~1.20ml / L and the rest; low molecular weight carbon-containing organic matter is ethanol, DL-malic acid or glucose; ammonium salt is NH 4 Cl or (NH 4 ) 2 SO 4 ; The trace element is Zn 2+ , Mn 2+ 、Cu 2+ 、Co 2+ and Fe 2+ ; The pH value of the medium is 5.5 to 7.5.

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specific Embodiment approach 2

[0011] Specific embodiment two: what this embodiment is different from specific embodiment one is that the carbon content in the low molecular weight carbon-containing organic matter in every 1L photosynthetic bacteria culture medium is 1.00g / L, the nitrogen content in the ammonium salt is 2.00g / L, The content of yeast extract is 0.50g / L, MgSO 4 The content is 0.12g / L, CaCl 2 The content is 0.75g / L, K 2 HPO 4 The content is 0.50g / L, KH 2 PO 4 Distilled water with content of 0.30g / L, trace element content of 1.00ml / L and balance. Others are the same as in the first embodiment.

[0012] In this embodiment, the trace elements can not only meet the growth requirements of the photosynthetic bacteria, but also ensure the bright color of the bacteria solution of the photosynthetic bacteria.

specific Embodiment approach 3

[0013] Specific embodiment three: the difference between this embodiment and specific embodiment one is that Zn in the trace element 2+ The concentration is 0.001~0.005mol / L, Mn 2+ The concentration is 0.005~0.015mol / L, Cu 2+ The concentration is 0.001~0.005mol / L, Co 2+ The concentration is 5.00×10 -4 ~10.00×10 -4 mol / L,Fe 2+ The concentration is 0.001~0.005mol / L. Other steps are the same as in the first embodiment.

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Abstract

The invention discloses a photosynthetic bacteria culture medium, relates to a bacteria culture medium, and solves the problems that the existing photosynthetic bacteria culture media contains high-concentration yeast extract that leads to darker lysate color and high production cost, and pH value of the culture medium rises along with the prolonging of cultivation time, thus inhibiting the growth of photosynthetic bacteria and leading to lower bacteria concentration. The photosynthetic bacteria culture medium is made of low molecular weight carbonaceous organic substances, ammonium salts, the yeast extract, MgSO4, CaCl2, K2HPO4, KH2PO4, trace elements and distilled water. As the photosynthetic bacteria culture medium takes the low molecular weight carbonaceous organic substances that do not contain Na<+> as carbon source, pH value of the culture medium does not rise along with the prolonging of cultivation time, and the growth of photosynthetic bacteria is not inhibited; low-concentration yeast extract is adopted to reduce production cost, and the trace elements ensure bright photosynthetic bacteria lysate color; photosynthetic bacteria cultivated by the culture medium has the exponential growth phase of two days, thus having high bacteria concentration and the OD660 of 1.8.

Description

technical field [0001] The invention relates to a bacterial culture medium. Background technique [0002] Photosynthetic bacteria is a general term for a special physiological group of prokaryotes that can carry out photosynthesis without producing oxygen. It belongs to a class of microorganisms in the hydrosphere and widely exists in various water bodies in nature. Photosynthetic bacteria cells are non-toxic, rich in nutrients, protein content as high as 60% to 65%, and contain a variety of B vitamins, pantothenic acid, coenzyme Q and other physiologically active substances. Photosynthetic bacteria are widely used in aquaculture, planting, sewage treatment and so on. Photosynthetic bacteria cells are rich in bacteriochlorophyll and carotenoids, which are easy to extract and are new resources of natural food pigments, which can be used for coloring treatment of butter, ice cream, cheese, lactic acid drinks, pastries, etc. [0003] At present, the concentration of yeast ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 张光明何春华
Owner HARBIN INST OF TECH
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