33 results about "Bacteriochlorophyll" patented technology

A method of making a bacteriochlorin is carried out by condensing a pair of compounds of Formula II to produce the bacteriochlorin, wherein R is an acetal or aldehyde group. The condensing may be carried out in an organic solvent, preferably in the presence of an acid. The bacteriochlorins are useful for a variety of purposes such as active agents in photodynamic therapy, luminescent compounds in flow cytometry, solar cells, light harvesting arrays, and molecular memory devices.

Bacteriochlorin of Formula I: wherein R is H or silyl are described, along with compositions containing the same and methods of making and using the same.

A method of making a bacteriochlorin is carried out by condensing a pair of compounds of Formula IIto produce the bacteriochlorin, wherein R is an acetal or aldehyde group. The condensing may be carried out in an organic solvent, preferably in the presence of an acid. The bacteriochlorins are useful for a variety of purposes such as active agents in photodynamic therapy, luminescent compounds in flow cytometry, solar cells, light harvesting arrays, and molecular memory devices.

The invention provides cationic tetracyclic and pentacyclic bacteriochlorophyll derivatives (Bchls) containing at least one positively charged group and / or at least one basic group that is converted to a positively charged group under physiological conditions, preferably Bchls having an onium group derived from a N-containing aliphatic or heterocyclic radical such as ammonium, guanidinium, imidazolium, pyridinium, and the like or a phosphonium, arsonium, oxonium, sulfonium, selenonium, telluronium, stibonium, or bismuthonium group, or a basic group that is converted to such onium groups under physiological conditions, said groups being bound to one or more of the positions 17<3>, 13<3> and 3<2> of the Bchl molecule by ester or amide bond. The Bchls are useful for photodynamic therapy and diagnosis.

Provided are fluorescent micro particles and / or nano particles that have a polymeric matrix and at least one porphyrinic macrocycle associated therewith. The porphyrinic macrocycles associated with the presently disclosed fluorescent micro particles and / or nano particles are in some embodiments selected from the group consisting of porphyrins (including 17, 18-didehydrophorbines), chlorins (including phorbines), bacteriochlorins (including bacteriophorbines), and isobacteriochlorins (including isobacteriochlorins containing a fused E ring). In some embodiments, the porphyrinic macrocycles havestructures selected from Formulas I and H. Also provided are populations made up of a plurality of distinct fluorescent micro particles and / or nano particles, methods of making and using the same, sets of separately detectable fluorescent micro particles and / or nano particles and methods for preparing and using the same, porphyrinic macrocycle-containing dyads conjugated to particles and methodsfor using the same to calibrate multi-laser flow cytometers, align and / or calibrate confocai fluorescence microscopes, and / or differentially labeling cells and / or other bio-molecules, and bio-molecules such as antibodies, fragments, and derivatives thereof covalently conjugated to the presently disclosed fluorescent micro particles and / or nano particles and / or to the presently disclosed porphyrinic macrocycle-containing dyads.

A method of making a bacteriochlorin is carried out by condensing a pair of compounds of Formula IIto produce the bacteriochlorin, wherein R is an acetal or aldehyde group. The condensing may be carried out in an organic solvent, preferably in the presence of an acid. The bacteriochlorins are useful for a variety of purposes such as active agents in photodynamic therapy, luminescent compounds in flow cytometry, solar cells, light harvesting arrays, and molecular memory devices.

A compound comprising a chemical combination of a photodynamic tetra-pyrrolic compound with a plurality of radionuclide element atoms such that the compound may be used to enhance MR imaging and also be used as a photodynamic compound for use in photodynamic therapy to treat hyperproliferative tissue. The preferred compounds have the structural formula: where R1, R2, R2a R3, R3a R4, R5, R5a R6, R7, R7a, and R8 cumulatively contain at least two functional groups that will complex or combine with an MR imaging enhancing element or ion. The compound is intended to include such complexes and combinations and includes the use of such compounds for MR imaging and photodynamic therapy treatment of tumors and other hyperproliferative tissue.

RGD-chlorophyll and RGD-bacteriochlorophyll conjugates that home and accumulate in necrotic tumor domains much longer than in tumor non-necrotic domains are provided for use in minimally invasive tumor-targeted imaging, tumor-targeted photodynamic therapy, and / or on-line prognosis of necrotic tumors.

The invention relates to a removing method for nitrate in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of herba houttuyniae, herba incarvller and kudzu are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, protein and pectin are added and mixed with the mixture to be even, titration is performed through diethyl ether, and filtration is performed through a filter membrane for sterilization; sodium dihydrogen phosphate and magnesium chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; succinic acid is added to adjust pH; methanol and ethyl acetate are added into the mixed liquid and stirred, in addition, glutamic acid is added, sterilization is performed, pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium thiosulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Equipment is low in manufacturing cost, running costs are low, and management is easy and convenient; besides, phosphate removal is efficient, low in consumption and free of secondary pollution.

The invention discloses an environment-friendly method for preparing anoxygenic photosynthetic bacterium carotenoids. The method comprises the steps of collecting wet thalli of anoxygenic photosynthetic bacteria, adding the wet thalli to an alkaline low-concentration ethanol-water solution, selectively extracting and removing bacteriochlorophyll rather than the carotenoids in the wet thalli, and extracting the carotenoids in the wet thalli through absolute ethyl alcohol after removing the bacteriochlorophyll. According to the method, the anoxygenic photosynthetic bacterium carotenoids are extracted according to the ethanol-water process, so that the selectivity is good, the purity of the carotenoids is high, operation is easy, and conditions are mild; no other organic solvents are adopted in the whole process except ethanol and water, so that harmful residues of the organic solvents are reduced, the method is environmentally friendly, operation is easy and convenient, and scale production can be easily achieved.

The invention relates to synthesis of a biological agent for gathering nitrite in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of lalang grass rhizome, selaginella uncinata and monstera rhizome are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, agar and gelatin are added and mixed to be even, titration is performed through ethyl acetate, and filtration is performed through a filter membrane for sterilization; sodium hexametaphosphate and zinc chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; an aluminum sulfate solution is added to adjust pH; formaldehyde and acetone are added into the mixed liquid and stirred, in addition, threonine is added, sterilization is performed, the pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium sulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Raw materials are wide in source and convenient and easy to obtain, the technology is simple, and operation costs are low.

The invention relates to a removing method for nitrate in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of herba houttuyniae, herba incarvller and kudzu are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, protein and pectin are added and mixed with the mixture to be even, titration is performed through diethyl ether, and filtration is performed through a filter membrane for sterilization; sodium dihydrogen phosphate and magnesium chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; succinic acid is added to adjust pH; methanol and ethyl acetate are added into the mixed liquid and stirred, in addition, glutamic acid is added, sterilization is performed, pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium thiosulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Equipment is low in manufacturing cost, running costs are low, and management is easy and convenient; besides, phosphate removal is efficient, low in consumption and free of secondary pollution.

The invention provides anionic water-soluble tetracyclic and pentacyclic bacteriochlorophyll derivatives (Bchls) containing at least one, preferably two or three, negatively charged groups and / or acidic groups that are converted to negatively charged groups at the physiological pH, preferably Bchls having a group COO<−>, COS<−>, SO3<−>, PO3<2−>, COOH, COSH, SO3H, and / or PO3H2 bound through an ester or amide bond to one or more of the positions 17<3>, 13<3>, and 3<2> of the tetracyclic or pentacyclic Bchl molecule, for photodynamic therapy and diagnosis.

A compound that is a conjugate of an antagonist to an integrin expressed by a tumor cell and at least one of a tumor avid tetrapyrollic photosensitizer, a fluorescent dye, and a radioisotope labeled moiety wherein the radioisotope is 11C, 18F, 64Cu, 124I, 99Tc, 111In or GdIII and its method of use for diagnosing, imaging and / or treating hyperproliferative tissue such as tumors. Preferably the photosensitizer is a tumor avid tetrapyrollic photosensitizer, e.g. a porphyrin, chlorin or bacteriochlorin, e.g. pheophorbides and pyropheophorbides. Such conjugates have extreme tumor avidity and can be used to inhibit or completely destroy the tumor by light absorption. The integrin is usually αvβ3, α5β1, αvβ5, α4β1, or α2β1. Preferably, the antagonist is an RGD peptide or another antagonist that may be synthetic such as a 4-{2-(3,4,5,6-tetra-hydropyrimidin-2-ylamino)ethyloxy}-benzoyl]amino-2-(S)-amino-ethyl-sulfonylamino group. Such compounds provide tumor avidity and imaging ability thus permitting selective and clear tumor imaging.