Hydroporphyrin beads with narrow fluorescence emissions

Abstract

Provided are fluorescent micro particles and / or nano particles that have a polymeric matrix and at least one porphyrinic macrocycle associated therewith. The porphyrinic macrocycles associated with the presently disclosed fluorescent micro particles and / or nano particles are in some embodiments selected from the group consisting of porphyrins (including 17, 18-didehydrophorbines), chlorins (including phorbines), bacteriochlorins (including bacteriophorbines), and isobacteriochlorins (including isobacteriochlorins containing a fused E ring). In some embodiments, the porphyrinic macrocycles havestructures selected from Formulas I and H. Also provided are populations made up of a plurality of distinct fluorescent micro particles and / or nano particles, methods of making and using the same, sets of separately detectable fluorescent micro particles and / or nano particles and methods for preparing and using the same, porphyrinic macrocycle-containing dyads conjugated to particles and methodsfor using the same to calibrate multi-laser flow cytometers, align and / or calibrate confocai fluorescence microscopes, and / or differentially labeling cells and / or other bio-molecules, and bio-molecules such as antibodies, fragments, and derivatives thereof covalently conjugated to the presently disclosed fluorescent micro particles and / or nano particles and / or to the presently disclosed porphyrinic macrocycle-containing dyads.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Patent Application Serial No. 62 / 348,532, filed June 10, 2016, the disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The presently disclosed subject matter relates to compounds comprising one or more porphyrin macrocycles, optionally one or more chlorins, bacteriochlorins and / or isobacteriochlorins Compositions, and methods of labeling and / or detecting biomolecules and / or cells using the same. In particular, it relates to compositions comprising the porphyrin macrocycles disclosed herein, molecules and carriers such as nanoparticles and microparticles (which have been labeled with the porphyrin macrocycles disclosed herein), and the use thereof to label and / or detect targets Methods. Background technique [0004] Fluorescence-based polychromatic flow cytometry (PFC) is a key analytical technique in modern immunology...

AI Technical Summary

Problems solved by technology

These practices result in reduced fluorescence sensitivity and artifacts such as "diffusion"

For example, even with a prototype 50-color instrument, leading PFC researchers were only able to achieve a 30-parameter panel (Chattopadhyay et al., 2015)

Images