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Environment-friendly method for preparing anoxygenic photosynthetic bacterium carotenoids

A technology of photosynthetic bacteria and carotene, applied in the field of microbiology, can solve problems affecting food and drug safety, large amount of organic solvents, solvent residues, etc., and achieve the effects of easy acquisition, thorough separation, and simple operation

Active Publication Date: 2014-08-27
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the current preparation methods all extract carotenoids and bacteriochlorophyll in the bacteria simultaneously, and then obtain carotenoids through saponification-extraction or column chromatography, although the extraction and saponification processes can be carried out simultaneously, simplifying However, both the saponification extraction process and the column chromatography process involve the use of organic solvents other than ethanol and water. Especially in large-scale production, the amount of organic solvents is large, which not only pollutes the environment, but also It will inevitably bring solvent residues, which will affect the subsequent food and drug safety

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of austenone carotenoids from the marine Chromobacterium 283-1 strain:

[0022] 1) Take 4 equivalents of 283-1 wet bacteria cultured under anaerobic light for 5 days, each 2.0g, add 10ml containing 5.0% (w / v) NaOH at a bath ratio of 1:5 (w / v) 25, 30, 40 and 50% (v / v) ethanol aqueous solution, suspend the cells and shake well, extract at room temperature for 10.0 minutes, collect the wet bacteria by centrifugation, and repeat the extraction once to selectively remove the wet bacteria Bacteriochlorophyll in the body;

[0023] 2) Collect the wet cells from which bacteriochlorophyll has been removed, add 10ml of distilled water at a bath ratio of 1:5 (w / v), wash the cells three times until the pH of the cells is 6.8-7.1, and use a bath ratio of 1:16 ( w / v) Add 32ml of absolute ethanol, extract at room temperature in the dark for 40.0 minutes, repeat the extraction of the wet bacteria twice in the dark, combine the extracts, recover the solvent at 30°C to 50°C, ...

Embodiment 2

[0026] Preparation of spirulina xanthophyll carotenoids from Rhodopseudomonas palustris strain CQV97:

[0027] 1) Take 2.0g of CQV97 wet bacteria, add 8ml of 40% (v / v) ethanol aqueous solution containing 10.0% (w / v) KOH according to the bath ratio of 1:4 (w / v), suspend the cells and shake well Afterwards, leaching at room temperature for 40.0 minutes selectively extracts and removes the bacteriochlorophyll in the wet thalline;

[0028] 2) Collect the wet cells from which bacteriochlorophyll has been removed, add 10ml of distilled water according to the bath ratio of 1:5 (w / v), and add an appropriate amount of 1mol / L HCl to adjust the pH value of the cells to 6.8-7.1, according to the bath ratio of 1:8 (w / v) Add 16ml of absolute ethanol, extract at room temperature for 3 hours in the dark, recover the solvent at 30°C to 50°C, concentrate and dry to obtain carotenoids.

[0029] After testing, the amount of carotenoids extracted from wet cells of Rhodopseudomonas palustris CQV97...

Embodiment 3

[0031] Preparation of spherical olefinic carotenoids from Rhodobacter nitrogen-fixing strain R7:

[0032] 1) Take 2.0g of R7 wet bacteria, add 60ml of 30% (v / v) ethanol aqueous solution containing 5.0% (w / v) NaOH according to the bath ratio of 1:30 (w / v), suspend the cells and shake well Afterwards, leaching at room temperature for 8.0 minutes, centrifuging to collect the wet cells, and repeating the extraction twice to selectively extract and remove the bacteriochlorophyll in the wet cells;

[0033]2) Collect the wet cells from which bacteriochlorophyll has been removed, add 10ml of distilled water at a bath ratio of 1:5 (w / v), wash the cells four times until the pH of the cells is 6.8-7.1, and use a bath ratio of 1:16 ( w / v) Add 32ml of absolute ethanol, extract for 2 hours at room temperature in the dark, then repeat the extraction twice for the wet cells in the dark, combine the extracts, add an appropriate amount of protective agent vitamin C, recover the solvent at 30°C ...

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Abstract

The invention discloses an environment-friendly method for preparing anoxygenic photosynthetic bacterium carotenoids. The method comprises the steps of collecting wet thalli of anoxygenic photosynthetic bacteria, adding the wet thalli to an alkaline low-concentration ethanol-water solution, selectively extracting and removing bacteriochlorophyll rather than the carotenoids in the wet thalli, and extracting the carotenoids in the wet thalli through absolute ethyl alcohol after removing the bacteriochlorophyll. According to the method, the anoxygenic photosynthetic bacterium carotenoids are extracted according to the ethanol-water process, so that the selectivity is good, the purity of the carotenoids is high, operation is easy, and conditions are mild; no other organic solvents are adopted in the whole process except ethanol and water, so that harmful residues of the organic solvents are reduced, the method is environmentally friendly, operation is easy and convenient, and scale production can be easily achieved.

Description

technical field [0001] The invention relates to the technical field of microbiology, in particular to an environmentally friendly method for preparing carotenoids from photosynthetic bacteria that do not produce oxygen. Background technique [0002] Carotenoids are not only rich in color, but also have physiological activities such as anti-oxidation, free radical scavenging, immune regulation, regulation of cell proliferation and differentiation, signal transduction, and intercellular gap junction communication. Disease prevention and health care can even inhibit the occurrence and growth of tumors, and have a good preventive effect on eye diseases and cardiovascular diseases. Therefore, carotenoids are widely used in medicine, health care, cosmetics, beauty, nutritional food and feed additives. Due to the toxic and side effects of chemically synthesized pigments, the use of various chemically synthesized carotenoids has been restricted or prohibited, and the carotenoids fr...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 杨素萍赵春贵贾亚琼
Owner HUAQIAO UNIVERSITY
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