Synthesis of biological agent for gathering nitrite in micro-polluted water body

Abstract

The invention relates to synthesis of a biological agent for gathering nitrite in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of lalang grass rhizome, selaginella uncinata and monstera rhizome are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, agar and gelatin are added and mixed to be even, titration is performed through ethyl acetate, and filtration is performed through a filter membrane for sterilization; sodium hexametaphosphate and zinc chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; an aluminum sulfate solution is added to adjust pH; formaldehyde and acetone are added into the mixed liquid and stirred, in addition, threonine is added, sterilization is performed, the pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium sulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Raw materials are wide in source and convenient and easy to obtain, the technology is simple, and operation costs are low.

Description

technical field [0001] The invention relates to the synthesis of a biological agent for enriching the content of nitrite in slightly polluted water, and belongs to the field of sewage treatment. Background technique [0002] Nitrite exists widely in water bodies, and its severe biological toxicity has attracted more and more attention. The toxicity mechanism of nitrite, the biological toxicity and removal research status of nitrite in water were summarized, and the future research and utilization direction of nitrite in drinking water treatment were proposed. Nitrite is a strong blood toxin. After it enters the human body in large quantities, it can oxidize the ferrous iron in hemoglobin to ferric iron, causing the blood to lose its oxygen-carrying function, leading to hypoxia and suffocation of the human body, causing dyspnea, and circulatory problems. Exhaustion, damage to the central nervous system; Nitrite has another dangerous potential toxicity, which can be combined ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of incomplete removal, difficult operation and management, and inability to be widely used in traditional methods, the present invention provides a synthesis of biological preparations for enriching nitrite content in slightly polluted water bodies. A mixture of chlorophyll a and bacteriochlorophyll is extracted from herbaceous plants such as bamboo rhizomes, dissolved in a mixed solution of sodium hexametaphosphate and zinc chloride, and finally a biological agent with low consumption and high removal rate is obtained