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A kind of biological preparation method for enriching the content of nitrite in slightly polluted water body

A technology of nitrite and biological preparations, applied in water pollutants, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as inability to be widely used, difficult operation and management, incomplete removal, etc., and achieve no secondary The effect of pollution, wide application range and low operating cost

Active Publication Date: 2015-11-18
福建龙骏环保设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of incomplete removal, difficult operation and management, and inability to be widely used in traditional methods, the present invention provides a synthesis of biological preparations for enriching nitrite content in slightly polluted water bodies. A mixture of chlorophyll a and bacteriochlorophyll is extracted from herbaceous plants such as bamboo rhizomes, dissolved in a mixed solution of sodium hexametaphosphate and zinc chloride, and finally a biological agent with low consumption and high removal rate is obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Take 8g of Imperata rhizome, 6g of Cuiyuncao, and 10g of Monstera rhizome and grind them in a Webster blender, add 10mL of absolute ethanol, pass through the chromatography column, use sodium bicarbonate powder as the stationary phase, and ethyl acetate as the mobile phase Separate and extract the mixture of chlorophyll a and bacteriochlorophyll; put the above mixture in a 50mL stoppered colorimetric tube, place it in a constant temperature water bath at 50°C for 2h, add 3g agar and 1g gelatin, mix well, and transfer to a 100mL volumetric flask , dilute to volume with ethyl acetate, and then sterilize with a 0.05 μm membrane filter; dissolve 2 g of sodium hexametaphosphate and 1.0 g of zinc chloride in 10 mL of deionized water, stir, and sterilize under high temperature and high pressure for 20 min after completely dissolving, then cool to 60°C, and then added to the mixed solution of chlorophyll a and bacteriochlorophyll after filter membrane sterilization; when the sol...

example 2

[0036] Take 12g of Imperata imperinae, 9g of Cuiyuncao, and 14g of Monstera rhizome and grind them in a Webster blender, add 15mL of absolute ethanol, pass through the chromatography column, use sodium bicarbonate powder as the stationary phase, and ethyl acetate as the mobile phase Separate and extract the mixture of chlorophyll a and bacteriochlorophyll; put the above mixture in a 50mL stoppered colorimetric tube, place it in a constant temperature water bath at 75°C for 2 hours, add 4.5g agar and 1.5g gelatin, mix well, and transfer to a capacity of 100mL In the bottle, dilute to volume with ethyl acetate, and then filter and sterilize with a 0.75μm filter membrane; dissolve 2.5g sodium hexametaphosphate and 2.0g zinc chloride in 10mL deionized water, stir, and sterilize under high temperature and high pressure after completely dissolving 20min, cooled to 80°C, and then added to the mixture of chlorophyll a and bacteriochlorophyll after filter membrane sterilization; when th...

example 3

[0040] Take 16g of Imperata imperinae, 12g of Cuiyuncao, and 18g of Monstera rhizome and grind them in a Webster blender, add 20mL of absolute ethanol, and pass through the chromatography column, using sodium bicarbonate powder as the stationary phase, and ethyl acetate as the mobile phase Separate and extract the mixture of chlorophyll a and bacteriochlorophyll; put the above mixture in a 50mL stoppered colorimetric tube, place it in a constant temperature water bath at 100°C for 2 hours, add 6g agar and 2g gelatin, mix well, and transfer to a 100mL volumetric flask , dilute to volume with ethyl acetate, then filter and sterilize with 0.10μm filter membrane; dissolve 3g sodium hexametaphosphate and 3.0g zinc chloride in 10mL deionized water, stir, after completely dissolving, sterilize under high temperature and high pressure for 20min, cool to 100°C, and then added to the mixed solution of chlorophyll a and bacteriochlorophyll after filter membrane sterilization; when the sol...

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Abstract

The invention relates to synthesis of a biological agent for gathering nitrite in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of lalang grass rhizome, selaginella uncinata and monstera rhizome are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, agar and gelatin are added and mixed to be even, titration is performed through ethyl acetate, and filtration is performed through a filter membrane for sterilization; sodium hexametaphosphate and zinc chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; an aluminum sulfate solution is added to adjust pH; formaldehyde and acetone are added into the mixed liquid and stirred, in addition, threonine is added, sterilization is performed, the pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium sulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Raw materials are wide in source and convenient and easy to obtain, the technology is simple, and operation costs are low.

Description

technical field [0001] The invention relates to a method for synthesizing biological preparations for enriching nitrite content in slightly polluted water bodies, belonging to the field of sewage treatment. Background technique [0002] Nitrite exists widely in water bodies, and its severe biological toxicity has attracted more and more attention. The toxicity mechanism of nitrite, the biological toxicity and removal research status of nitrite in water were summarized, and the future research and utilization direction of nitrite in drinking water treatment were proposed. Nitrite is a strong blood toxin. After it enters the human body in large quantities, it can oxidize the ferrous iron in hemoglobin to ferric iron, causing the blood to lose its oxygen-carrying function, leading to hypoxia and suffocation of the human body, causing dyspnea, and circulatory problems. Exhaustion, damage to the central nervous system; Nitrite has another dangerous potential toxicity, which can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/32C02F101/16
Inventor 赵远
Owner 福建龙骏环保设备有限公司
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