Synthesis of biological agent for gathering nitrate in micro-polluted water body

Abstract

The invention relates to a removing method for nitrate in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of herba houttuyniae, herba incarvller and kudzu are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, protein and pectin are added and mixed with the mixture to be even, titration is performed through diethyl ether, and filtration is performed through a filter membrane for sterilization; sodium dihydrogen phosphate and magnesium chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; succinic acid is added to adjust pH; methanol and ethyl acetate are added into the mixed liquid and stirred, in addition, glutamic acid is added, sterilization is performed, pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium thiosulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Equipment is low in manufacturing cost, running costs are low, and management is easy and convenient; besides, phosphate removal is efficient, low in consumption and free of secondary pollution.

Description

technical field [0001] The invention relates to the synthesis of a biological agent for enriching the nitrate content in slightly polluted water, and belongs to the field of sewage treatment. Background technique [0002] Groundwater is an important source of water, even the only source of drinking water for many people. However, whether in industrially developed countries or developing countries, due to the application of a large amount of nitrogen fertilizers in rural areas, the discharge of domestic sewage and nitrogen-containing industrial wastewater does not meet the standards And leakage, leaching and infiltration of solid waste, unreasonable recharge of sewage, and excessive exploitation of groundwater, etc., lead to the increase of nitrate concentration in groundwater, which has become an environmental problem. Nitrate is the precursor of ammonium nitrite, a strong carcinogen. Excessive intake of drinking water with high nitrate content will cause harm to human heal...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at problems such as incomplete removal, secondary pollution, and difficulty in operation and management in traditional methods. The present invention provides a method for enriching nitrate in slightly polluted water bodies content of biological preparations synthesis, the present invention uses Houttuynia cordata, sweet potato vine, kudzu vine and other herbal plants as raw materials to extract the mixture of chlorophyll a and bacteriochlorophyll, dissolves in the mixed solution of sodium dihydrogen phosphate and magnesium chloride, and finally obtains low-consumption, Biological agents with high removal rate