Synthesis of biological agent for gathering nitrate in micro-polluted water body

A technology of nitrate content and biological agents, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., to achieve low cost, low operating cost, and easy management

Active Publication Date: 2014-11-12
广州贝山水生态科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at problems such as incomplete removal, secondary pollution, and difficulty in operation and management in traditional methods. The present invention provides a method for enriching nitrate in slightly polluted water bodies content of biological preparations synthesis, the present invention uses Houttuynia cordata, sweet potato vine, kudzu vine and other herbal plants as raw materials to extract the mixture of chlorophyll a and bacteriochlorophyll, dissolves in the mixed solution of sodium dihydrogen phosphate and magnesium chloride, and finally obtains low-consumption, Biological agents with high removal rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Take 5g of Houttuynia cordata, 10g of sweet potato vine, and 5g of kudzu vine and crush them in a Webster blender, add 10mL of absolute ethanol, pass through the chromatography column, use calcium carbonate particles as the stationary phase, and acetone as the mobile phase to separate and extract chlorophyll a and bacteriochlorophyll mixture; put the above mixture in a 50mL stoppered colorimetric tube, place it in a constant temperature water bath at 60°C for 1.5h, add 1g protein and 0.5g pectin, mix well, transfer to a 100mL volumetric flask, and Dilute to volume with diethyl ether, then filter and sterilize with 0.05um filter membrane; dissolve 1.0g sodium dihydrogen phosphate and 0.5g magnesium chloride in 10mL deionized water, stir, after completely dissolving, sterilize under high temperature and high pressure for 30min, cool to 60°C, and then Add chlorophyll a and bacteriochlorophyll mixture after filter membrane sterilization; when the solution is cooled to room t...

example 2

[0036]Take 7g of Houttuynia cordata, 11g of sweet potato vine, and 6.5g of kudzu vine and grind them in a Webster blender, add 12.5mL of absolute ethanol, pass through the chromatography column, use calcium carbonate particles as the stationary phase, and acetone as the mobile phase to separate and extract Produce a mixture of chlorophyll a and bacteriochlorophyll; put the above mixture in a 50mL stoppered colorimetric tube, place it in a constant temperature water bath at 70°C for 1.5h, add 1.5g protein and 0.75g pectin, mix well, and transfer to a 100mL volumetric flask In the solution, dilute to volume with ether, then filter and sterilize with a 0.075um filter membrane; dissolve 1.25g of sodium dihydrogen phosphate and 0.75g of magnesium chloride in 10mL of deionized water, stir, and sterilize at high temperature and high pressure for 30 minutes after complete dissolution, and cool at 70° C, then add the chlorophyll a and bacteriochlorophyll mixture after filter membrane st...

example 3

[0040] Take a certain amount of Houttuynia cordata, sweet potato vine, and kudzu vine and crush them in a Webster blender, add absolute ethanol, pass through a chromatographic column, use calcium carbonate particles as the stationary phase, and acetone as the mobile phase to separate and extract chlorophyll a and Bacterial chlorophyll mixture: put the above mixture in a stoppered colorimetric tube, place it in a constant temperature water bath, add protein and pectin, mix well, transfer to a volumetric flask, dilute with ether, and then filter to sterilize; Dissolve sodium dihydrogen phosphate and magnesium chloride in deionized water, stir, sterilize under high temperature and high pressure after being completely dissolved, cool, and then add the mixed solution of chlorophyll a and bacteriochlorophyll after filter membrane sterilization; when the solution is cooled to room temperature, Add 1 succinic acid to adjust pH = 6.0; add methanol and ethyl acetate to the above mixture,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a removing method for nitrate in a micro-polluted water body and belongs to the field of wastewater treatment. The method comprises the steps that a certain amount of herba houttuyniae, herba incarvller and kudzu are taken and smashed, absolute ethyl alcohol is added, the mixture passes through a chromatographic column, and a mixture composed of chlorophyll a and bacteriochlorophyll is extracted; the mixture is placed into a colorimetric tube with a plug, protein and pectin are added and mixed with the mixture to be even, titration is performed through diethyl ether, and filtration is performed through a filter membrane for sterilization; sodium dihydrogen phosphate and magnesium chloride are dissolved in de-ionized water, and after being stirred and cooled, the solution is added into the chlorophyll a and bacteriochlorophyll mixed liquid sterilized through the filter membrane; succinic acid is added to adjust pH; methanol and ethyl acetate are added into the mixed liquid and stirred, in addition, glutamic acid is added, sterilization is performed, pH is adjusted, cooling is performed, and sterile filtration is performed to obtain colorless supernatant liquid; a sodium thiosulfate solution and a magnesium chloride solution are added into the supernatant liquid, pH is adjusted, and the mixed liquid is cooled, sterilized, mixed and then poured into a sterilized test tube for low-temperature storage out of the sun. Equipment is low in manufacturing cost, running costs are low, and management is easy and convenient; besides, phosphate removal is efficient, low in consumption and free of secondary pollution.

Description

technical field [0001] The invention relates to the synthesis of a biological agent for enriching the nitrate content in slightly polluted water, and belongs to the field of sewage treatment. Background technique [0002] Groundwater is an important source of water, even the only source of drinking water for many people. However, whether in industrially developed countries or developing countries, due to the application of a large amount of nitrogen fertilizers in rural areas, the discharge of domestic sewage and nitrogen-containing industrial wastewater does not meet the standards And leakage, leaching and infiltration of solid waste, unreasonable recharge of sewage, and excessive exploitation of groundwater, etc., lead to the increase of nitrate concentration in groundwater, which has become an environmental problem. Nitrate is the precursor of ammonium nitrite, a strong carcinogen. Excessive intake of drinking water with high nitrate content will cause harm to human heal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32
Inventor 雷春生
Owner 广州贝山水生态科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap