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Novel application of lotus plumule extracts

The technology of lotus seed heart and extract is applied in the field of medicine, and can solve the problems of not finding the bacterial quorum sensing system and the bacterial quorum sensing system, so as to achieve the effect of not easy bacterial drug resistance and good inhibitory effect.

Inactive Publication Date: 2018-04-24
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A systematic review of the literature on lotus seed heart found that modern pharmacology and clinical studies also showed that lotus seed heart has a strong inhibitory effect on common bacteria such as Pseudomonas aeruginosa, Salmonella bacillus, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. There is no literature report on its inhibition of bacterial quorum sensing system
[0007] The lotus seed extract contains a variety of chemical components, such as alkaloids, flavonoids, volatile oils, organic acids, etc., but it has not been found to inhibit the bacterial quorum sensing system

Method used

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  • Novel application of lotus plumule extracts
  • Novel application of lotus plumule extracts
  • Novel application of lotus plumule extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of lotus seed heart 80% ethanol primary extract and total alkaloids

[0043] (1) 10 kg of lotus seed heart (Plumula Nelambinis), soaked with 12 times the amount of 80% (v / v) ethanol overnight, then heated and refluxed for 3 times, the extraction temperature was 120 ° C, 2 hours each time, and the extracts were combined;

[0044] (2) remove insolubles by filtration, and dry the filtrate under reduced pressure to obtain 2.5 kg of lotus seed heart 80% ethanol initial extract;

[0045] (3) After dissolving 2.3 kg of 80% ethanol primary extract of lotus seed heart with an appropriate amount of 0.1% HCl, it is loaded on the pretreated D001 cation exchange resin. Use pure water, 95% ethanol solution (containing 1% NH 3 ) elution to obtain 700 g of water washing solution and 850 g of 95% alcohol washing solution. Among them, 95% alcohol washing solution (containing 1% NH 3 ) is mainly enriched in alkaloid compounds, that is, the total alkaloid e...

Embodiment 2

[0046] Embodiment 2, the preparation of lotus seed heart 60% ethanol primary extract and total flavonoids

[0047] (1) 5 kg of lotus seed heart (Plumula Nelambinis), soaked with 10 times the amount of 60% (v / v) ethanol overnight, then heated and refluxed for extraction 3 times, the extraction temperature was 130 ° C, 2 hours each time, and the extracts were combined;

[0048] (2) remove insolubles by filtration, and dry the filtrate under reduced pressure to obtain 1.6 kg of lotus seed heart 60% ethanol primary extract;

[0049] (3) After dissolving 1.5 kg of 60% ethanol initial extract of lotus seed heart with an appropriate amount of 0.1% HCl, it is loaded on the pretreated D001 cation exchange resin. Use pure water, 95% ethanol solution (containing 1% NH 3 ) elution to obtain water washing solution 500g and 95% alcohol washing solution 350g.

[0050] (4) After 450 g of washing liquid obtained is concentrated under reduced pressure, it is loaded on the pretreated AB-8 macr...

Embodiment 3

[0051] Embodiment 3, the preparation of lotus seed heart 80% ethanol initial extract and each separated component

[0052] (1) 8 kg of lotus seed heart (Plumula Nelambinis), soaked with 10 times the amount of 80% (v / v) ethanol overnight, then heated and refluxed for 3 times, the extraction temperature was 120 ° C, 2 hours each time, and the extracts were combined;

[0053] (2) remove insoluble matter by filtration, dry under reduced pressure, obtain 80% ethanol primary extract of lotus seed heart 2.1kg;

[0054] (3) Disperse 2.0 kg of the 80% ethanol initial extract of the lotus seed heart into water to form a 10L aqueous solution, extract three times with equal volumes of chloroform, ethyl acetate and n-butanol, and concentrate under reduced pressure to obtain 230 g of the lotus seed heart chloroform layer extract, Lotus seed heart ethyl acetate layer extract 320g, lotus seed heart n-butanol layer extract 450g, lotus seed heart aqueous layer extract 400g.

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Abstract

The application belongs to the field of medicine and discloses novel application of lotus plumule extracts, in particular to application of lotus plumule extracts in the preparation of inhibitory drugs for the quorum-sensing system. Experiments show that the lotus plumule extracts can inhibit the biological membrane of each of three species of Pseudomonas aeruginosa; that the lotus plumule extracts can inhibit the quorum-sensing system so as to decrease bacterial virulence and pathogenicity without inhibiting growth of bacteria, while bacterial drug resistance is rarely caused. Particularly all lotus plumule chloroform layer extract, lotus plumule ethyl acetate layer extract, lotus plumule total alkaloids extract and lotus plumule total flavones extract is as effective as or more effectivethan the positive drugs, furanone compounds, in inhibiting the biological membranes of Pseudomonas aeruginosa and drug-resistant Pseudomonas aeruginosa; it is indicated that the lotus plumule extracts provide good inhibition for both Pseudomonas aeruginosa and drug-resistant Pseudomonas aeruginosa.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the new application of the lotus seed extract, especially the application of the lotus seed extract in the preparation of inhibitory drugs for bacterial quorum sensing systems. Background technique [0002] With the widespread use of antibiotics, the resistance of microorganisms is getting higher and higher, and the resistance spectrum is getting wider and wider. The speed of drug resistance formation is proportional to the sterilization ability of antibiotics. Once resistance is established, it will persist. The continued use of antibiotics will only continue to provide selection pressure for highly drug-resistant strains, promoting their replication, organization, and sharing of drug-resistant genes, resulting in the accelerated formation of multi-drug resistant strains. At present, antibiotic resistance has become a serious public health problem worldwide. [0003] In my ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/62A61P31/04A61K131/00
CPCA61K36/62A61K2236/331A61K2236/333
Inventor 郑俊霞时伟朋田文月杨超邱美佩陈观桧何杰标赵肃清张焜
Owner GUANGDONG UNIV OF TECH
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